Transferases TRIZ Cases
- Host Cell Poison-Antidote System for Plasmid Stability
Chromosomal CcdB poison and plasmid CcdA antidote sequences eliminate antibiotic resistance markers while maintaining plasmid stability in E. coli.
- Multi-pass Transmembrane Polypeptide Aromatic Compound Production
Transformed cell expressing multi-pass transmembrane polypeptide produces aromatic compounds via shikimic acid pathway.
- Spontaneous Haploid Genome Doubling in Maize Breeding
Marker-assisted selection identifies maize genotypes with spontaneous haploid genome doubling capability for direct field sowing.
- Recombinant Yeast Isobutanol Biosynthetic Pathway
Engineered recombinant yeast converts plant-derived sugars into isobutanol, replacing petrochemical synthesis to lower costs and environmental impact.
- Mammalian Expression System with EBNA-1 and NDPK-A
Integrates EBNA-1 and NDPK-A to resolve inefficient nuclear DNA translocation, boosting transient expression yields.
- Engineered Prenyltransferase for Diverse Diketopiperazine Biosynthesis
Engineered prenyltransferase resolves substrate specificity limits by accepting multiple donors to synthesize diverse terpenylated diketopiperazines.
- EPSP Synthase Mutations for Glyphosate Resistance
Site-directed mutations in EPSP synthase reduce glyphosate binding affinity while maintaining catalytic activity, overcoming herbicide toxicity.
- Marker-Free Transgenic Plant DNA Constructs
Linked marker genes enable early phenotypic seed screening, replacing labor-intensive PCR analysis to remove unwanted DNA.
- Recombinant Yeast Strain for Bioethanol Production
A recombinant yeast strain propagates efficiently on low nutritive media using optimized gene copies.
- Recombinant Corynebacterium for 1,3-PDO Production
Deleting aldehyde dehydrogenase in recombinant Corynebacterium glutamicum reduces 3-hydroxypropionic acid byproduct formation during glycerol conversion.
- CRISPR-Cas9 Wx1 Gene Knockout for High Amylopectin Maize
CRISPR-Cas9 nuclease targets the Wx1 gene locus to generate high amylopectin maize, bypassing yield penalties from traditional breeding.
- Engineered Sulfotransferases Using Stable Aryl Sulfate Donors
Engineered sulfotransferases replace unstable PAPS with aryl sulfate donors, enabling reliable heparin production without decomposition.
- Enzymatic L-Methionine Synthesis via Methanethiol
Engineered methionine synthase converts O-phospho-L-homoserine and methanethiol into L-methionine, eliminating energy-intensive sulfur reduction steps.
- Malonyl-CoA Synthesis via Aminotransferase and Reductase Pathway
An artificial pathway synthesizes malonyl-CoA using beta-alanine precursors and heterologous enzymes.
- ATG Initiation Codon Substitution in Corynebacterium Glutamicum
Replacing GTG or TTG start signals with ATG boosts aspartate kinase and transketolase efficiency, increasing L-lysine yield by 4-12%.
- Corynebacterium Transformant for Catechol Production
A coryneform bacterium transformant produces catechol from saccharides using introduced decarboxylase genes.
- Whole-Cell Biocatalyst for Selective Amine Synthesis
Recombinant α-dioxygenase and transaminase convert carboxylic acids to amines, preventing over-oxidation by-products.
- DGAT Genes Enhance Triglyceride Production in Algae
Heterologous DGAT genes increase triglyceride yield while avoiding contamination risks from heterotrophic growth.
- Engineered Microorganisms for Alkene Production
Non-naturally occurring microbial organisms convert alcohols to alkenes via exogenous alkene pathway enzymes.
- DPO4 Polymerase Variants for Bulky Nucleotide Incorporation
Point mutations at position 78 in DPO4 polymerases enable accurate incorporation of bulky nucleotide analogs, reducing replication errors in homopolymer runs.
- Metabolically Engineered E. coli for Extracellular Heme Secretion
Engineered E. coli secretes heme via CcmABC transporters, resolving intracellular toxicity and boosting yields to 228 mg/L.