Fructus Trichosanthis Polypeptide Purification for Safer Testosterone Support
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Solution Overview
Problem
Current testosterone replacement therapies have side effects, and traditional Chinese medicines for tonifying kidney and invigorating yang are not safe for long-term use, while Fructus Trichosanthis seed has limited studies on its active substances and extraction challenges.
Innovation Solution
A method to prepare a Fructus Trichosanthis polypeptide by degreasing, enzymatic hydrolysis, and multi-stage purification to obtain a safe and effective polypeptide that accelerates testosterone secretion in TM3 cells, using a combination of neutral and alkaline proteases and chromatography techniques.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional Chinese medicines are used for tonifying kidney and invigorating yang, then the therapeutic effect is achieved, but safety is compromised due to side effects from long-term use
Solution Approach 1:
The patent extracts the active polypeptide components from Fructus Trichosanthis seed through degreasing, enzymatic hydrolysis, and purification processes. This isolation of specific bioactive substances eliminates the side effects associated with traditional herbal medicines while retaining the therapeutic effects of tonifying kidney and invigorating yang.
Solution Approach 2:
The patent transforms the traditional herbal medicine approach into a purified polypeptide product by changing the physical and chemical parameters during processing (degreasing, enzymatic hydrolysis, chromatography purification). This parameter transformation produces a safer, more controlled therapeutic agent with reduced side effects.
2Productivity
If Fructus Trichosanthis seed is used as raw material, then the source is abundant and nutritious, but the extraction process is complex and yield is low
Solution Approach 1:
The patent segments the preparation process into distinct sequential steps: degreasing to remove lipids, enzymatic hydrolysis to break down proteins into peptides, and chromatography for purification. This segmentation simplifies each individual step while achieving high overall yield and purity of the target polypeptide.
Solution Approach 2:
The patent performs preliminary degreasing and protein degradation steps before the main purification process. By pre-processing the Fructus Trichosanthis seed material to remove interfering substances and break down proteins, the subsequent purification steps become more efficient and yield higher quality polypeptide products.
3Reliability
If exogenous testosterone replacement therapy is administered, then testosterone deficiency is treated, but side effects occur
Solution Approach 1:
The patent uses Fructus Trichosanthis polypeptide as an intermediary substance that indirectly addresses testosterone deficiency. Rather than administering exogenous testosterone directly, the polypeptide acts as a mediator that supports endogenous testosterone production and sexual function, thereby avoiding the side effects of direct hormone replacement.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The Fructus Trichosanthis polypeptide is safe, well-absorbed, and significantly enhances testosterone secretion in TM3 cells, promoting mitochondrial biogenesis and providing a safer alternative for invigorating yang.
Implementation Method 1
performing enzymatic hydrolysis by composite enzyme to the Fructus Trichosanthis seed solution
Implementation Method 2
the composite enzyme comprises a neutral protease and an alkaline protease
Implementation Method 3
performing ion-exchange resin chromatography on a filtrate obtained
Implementation Method 4
a cellulose anion-exchange chromatographic column is used in the ion-exchange resin chromatography
Implementation Method 5
performing separation and purification by reversed-phase high-performance liquid chromatography (RP-HPLC)
Data Source
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Figure 3~4D
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AI summary
The present invention belongs to the field of biotechnology, and specifically relates to a Fructus Trichosanthis polypeptide having the efficacy of tonifying kidney yang, a preparation method and use thereof. The present invention prepares the Fructus Trichosanthis polypeptide having the efficacy of tonifying kidney and invigorating yang by processes such as pretreatment of a Fructus Trichosanthis raw material, preparation of a Fructus Trichosanthis seed solution, preparation of a crude Fructus Trichosanthis peptide, ion-exchange resin chromatography, and purification via high performance liquid chromatography. The Fructus Trichosanthis polypeptide provided by the present invention can modulate the expression of genes and proteins associated with secretion of testosterone in TM3 cells, enhance the function of mitochondria, accelerate the expression of genes and proteins associated with mitochondrial biogenesis, significantly promote the secretion of testosterone in TM3 cells, and has the efficacy of tonifying kidney and invigorating yang, thereby expanding the application range of Fructus Trichosanthis.