Universal Microbial Promoter for High-Efficiency Gene Expression

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Solution Overview

Problem

There is a need for a universal promoter system that exhibits high expression efficiency across various microorganisms, including Escherichia sp., Corynebacterium sp., and Bacillus sp., to enhance the production of target substances such as amino acids and other useful products.

Innovation Solution

A novel polynucleotide with promoter activity is developed, capable of expressing target genes in Corynebacterium, Escherichia, and Bacillus genera with higher efficiency than existing promoters, and can be used in expression cassettes and recombinant vectors to produce biologically active substances.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If a strong promoter is used in coryneform microorganisms, then gene expression efficiency is improved, but the general structure and mechanism of the promoter sequence remains unknown

Engineering Contradiction:

Improvegene expression efficiencyVSAvoidpromoter sequence structure information

Core Design Contradiction:

ProductivityVSLoss of information

Solution Approach 1:

The promoter was segmented into specific functional regions including a -35 region (sequence: 5'-TTGACA-3') and a -10 region (sequence: 5'-TATAAT-3'), allowing identification and characterization of each segment's contribution to transcription initiation and gene expression efficiency

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The promoter sequence parameters were optimized by modifying specific nucleotide sequences in the -35 and -10 regions to enhance RNA polymerase binding affinity and transcription initiation efficiency, resulting in improved gene expression while establishing a known structural framework

Inventive Principle:

Principle #35Parameter changes

2Reliability

If promoters are developed by deleting promoter parts of antibiotic resistant genes and transforming microorganisms, then some expression capability is achieved, but a universal high-efficiency expression system for multiple microorganisms is not established

Engineering Contradiction:

Improveexpression capabilityVSAvoiduniversal applicability across microorganisms

Core Design Contradiction:

ReliabilityVSAdaptability or versatility

Solution Approach 1:

The developed promoter sequence was designed and validated to function universally across multiple microorganism types including coryneform bacteria, Escherichia coli, and Bacillus species, enabling a single promoter construct to drive gene expression in diverse hosts without requiring host-specific modifications

Inventive Principle:

Principle #6Universality (Multi-functionality)

Solution Approach 2:

The promoter acts as an intermediary element that mediates transcription initiation across different microbial systems by providing conserved recognition sequences for RNA polymerase, facilitating universal gene expression without direct interaction with host-specific regulatory mechanisms

Inventive Principle:

Principle #24Intermediary (Mediator)

Data Source

PatentEP4647496A1Novel promoter and use thereof

Publication Date: 2025.11.12 CJ CHEILJEDANG CORP

AI summary

The present application relates to a novel promoter, and a method for preparing a target product using the same, and the polynucleotide according to one specific embodiment has a promoter activity, and is introduced into a microorganism, so it can increase expression and activity of a gene operatively linked thereto, and it can be usefully used for efficiently producing a target product affected by the polynucleotide and gene.