Galactose-1-Phosphate Uridyltransferase E414* Mutant for L-Lysine Yield
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for producing L-lysine using Corynebacterium glutamicum strains are limited by the production ability of the strains, necessitating improvements to enhance yield.
Innovation Solution
Incorporating a galactose-1-phosphate uridyltransferase mutant by knocking out or inhibiting the protein encoding gene, specifically mutating the glutamate residue codon at position 414 to a terminator or guanine nucleotide at position 1240, and using recombinant vectors to culture the modified bacteria for L-lysine production.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If the complete L-lysine biosynthesis pathway is used in Corynebacterium glutamicum, then L-lysine production is achieved, but the production yield is limited by the strain's inherent production ability
Solution Approach 1:
The invention changes the biochemical parameters of the strain by mutating the galactose-1-phosphate uridyltransferase enzyme (specifically the E414* mutation), which alters the metabolic flux distribution in the L-lysine biosynthesis pathway, thereby improving production yield
Solution Approach 2:
The invention extracts or removes the limiting factor by knocking out or inhibiting the galP gene encoding galactose-1-phosphate uridyltransferase, eliminating the metabolic bottleneck that constrained L-lysine production in the original strain
2Productivity
If galactose-1-phosphate uridyltransferase activity is maintained at normal levels, then metabolic pathway functionality is preserved, but L-lysine production efficiency is constrained
Solution Approach 1:
The invention applies local quality change by specifically mutating only the galactose-1-phosphate uridyltransferase enzyme (E414* mutation) while maintaining the functionality of other enzymes in the L-lysine biosynthesis pathway, creating a localized modification that improves overall production efficiency
Data Source
AI summary
Provided are a galactose-1-phosphate uridyltransferase mutant and the use thereof in the preparation of L-lysine. The galactose-1-phosphate uridyltransferase is A1) or A2): A1) a protein having an amino acid sequence as shown in SEQ ID No. 2; and A2) a fusion protein obtained by linking a tag to the N-terminal or/and the C-terminal of A1). Terminating or knocking out coding genes of the galactose-1-phosphate uridyltransferase in advance can improve the yield of L-lysine in cells.