Wharton's Jelly Stem Cell Collagen Production
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Solution Overview
Problem
Current methods for collagen production from animal tissues result in materials with unpleasant odors and tastes, and potential allergic reactions, while existing sources are limited and not biologically compatible with human biology.
Innovation Solution
A method involving the culture of Wharton's Jelly mesenchymal stem cells in specific media containing human basic fibroblast growth factor, proline, and L-ascorbic acid, followed by lysis and extraction using inorganic salt solutions, to produce collagen with enhanced regeneration and repair effects.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If collagen is extracted from animal tissues (pig skins, fish scales, internal organs), then collagen production is achieved, but the collagen has bad smells, bitter taste, and may induce allergic reactions
Solution Approach 1:
The patent uses Wharton's Jelly mesenchymal stem cells as an intermediary biological system to produce human collagen. Instead of directly extracting collagen from animal tissues, the invention cultures human stem cells that naturally produce human collagen, which is then harvested. This intermediary cellular system resolves the contradiction by providing a source of human-compatible collagen without the harmful characteristics of animal-derived collagen.
Solution Approach 2:
The patent changes the source parameter from animal tissues to human mesenchymal stem cells, and controls cultural conditions (medium composition, temperature, pH, oxygen concentration) to optimize collagen production. By adjusting these parameters, the invention achieves high-yield production of human collagen with desirable properties - eliminating bad smells, bitter taste, and allergic reactions while maintaining productivity.
2Quantity of substance
If collagen is extracted from animal tissues, then collagen material is obtained, but the material is not biologically compatible with human biology
Solution Approach 1:
The patent employs human Wharton's Jelly mesenchymal stem cells as an intermediary to produce human collagen. This cellular intermediary ensures that the collagen produced has human biological characteristics, making it compatible with human biology. The stem cells serve as a bridge between the production process and the desired human-compatible outcome, resolving the contradiction between obtaining sufficient collagen material and ensuring biological compatibility.
Solution Approach 2:
The patent changes the biological source parameter from animal to human cells, and optimizes cultural parameters (growth medium, temperature, pH, oxygen levels) to enhance collagen production. This parameter transformation achieves both objectives: sufficient collagen material production and high biological compatibility with human systems, making the collagen suitable for medical and cosmetic applications.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables the mass production of collagen with reduced allergenicity and odor, demonstrating enhanced collagen production and improved wound healing capabilities, suitable for medical and cosmetic applications.
Implementation Method 1
culturing the WJMSC cells in a first medium for 16 to 24 hours
Implementation Method 2
culturing the WJMSC cells in a first medium for 16 to 24 hours; replacing the first medium with a second medium for culturing the WJMSC cells for 36 to 48 hours
Implementation Method 3
adding a lysis solution to lyse the WJMSC cells for 0.5 to 2 hours
Implementation Method 4
adding an inorganic salt solution to the cell lysis solution to obtain a mixing solution, and incubating the mixed solution at 4° C. for 24 to 48 hours; spinning down the mixed solution and collecting a sediment
Data Source
AI summary
The present invention disclosed a method for preparing of collagen having regeneration and repair effects from Wharton's Jelly mesenchymal stein cells, comprising steps of culturing the Wharton's Jelly mesenchymal stein cells in a first medium for 16 to 24 hours; replacing the first medium with a second medium for culturing the Wharton's Jelly mesenchymal stein cells for 36 to 48 hours; collecting the Wharton's Jelly mesenchymal stein cells and adding a cell lysis solution to lyse the Wharton's Jelly mesenchymal stein cells for 0.5 to 2 hours, adding an inorganic salt solution to the cell lysis solution to obtain a mixing solution for further incubation at 4° C. for 24 to 48 hours; centrifuging the mixing solution and collecting a sediment, dissolving the sediment by a preservation solution to obtain a collage.