Dimming device, fluorescence microscopy optical system, and scanning and analysis system

Abstract

The embodiments of the present application provide a PWM dimming device, a fluorescence microscopy optical system, and a fluorescence-stained cell scanning and analysis system. The PWM dimming device includes: a voltage source; a PWM controller for controlling the on / off of the voltage source to output a pulsed voltage; an LED light source device to which the pulsed voltage is applied. The fluorescence microscopy optical system includes the above-mentioned PWM dimming device. The fluorescence-stained cell scanning and analysis system includes the above-mentioned PWM dimming device. The fluorescence-stained cell scanning and analysis system includes a fluorescence microscopy optical system. The embodiments of the present application solve the technical problems of low adjustment frequency and low adjustment accuracy of the traditional dimming device of the fluorescence-stained cell scanning and analysis system.

Description

Technical Field

[0001] This application relates to the field of fluorescence microscopy technology. Specifically, it relates to a PWM dimming device, a fluorescence microscopy optical system, and a fluorescence-stained cell scanning and analysis system. Background Art

[0002] In a fluorescence-stained cell scanning and analysis system, the traditional light source dimming method mainly includes the analog voltage regulation method. The principle of the analog voltage regulation method is as Figure 1 shown, including a voltage source 10, a variable resistor 20, and an LED light source device 30. By adjusting the resistance value of the variable resistor 20, the brightness of the LED light source device can be adjusted. This method requires the use of a variable resistor, and most variable resistors are mechanical structures, which have the disadvantages of low adjustment frequency, low adjustment accuracy, and poor reliability.

[0003] Therefore, the adjustment frequency and adjustment accuracy of the traditional dimming device are low, which is a technical problem that needs to be urgently solved by those skilled in the art.

[0004] The above information disclosed in the background art is only used to enhance the understanding of the background of this application. Therefore, it may contain information on prior art that is not known to those of ordinary skill in the art. Summary of the Invention

[0005] In the embodiments of this application, a PWM dimming device, a fluorescence microscopy optical system, and a fluorescence-stained cell scanning and analysis system are provided to solve the technical problem of low adjustment frequency and adjustment accuracy of the traditional dimming device.

[0006] An embodiment of this application provides a PWM dimming device for a fluorescence-stained cell scanning and analysis system, including:

[0007] A voltage source;

[0008] A PWM controller for controlling the on / off of the voltage source to output a pulsed voltage;

[0009] An LED light source device to which the pulsed voltage is applied.

[0010] An embodiment of this application also provides a fluorescence microscopy optical system including the above PWM dimming device.

[0011] An embodiment of this application also provides a fluorescence-stained cell scanning and analysis system including the above PWM dimming device.

[0012] An embodiment of this application also provides a fluorescence-stained cell scanning and analysis system including the above fluorescence microscopy optical system.

[0013] Due to the above technical solutions, the embodiments of this application have the following technical effects:

[0014] The on / off of the voltage source is controlled by a PWM controller to form a pulsed voltage and output the pulsed voltage. That is, the PWM controller can control the pulsed voltage applied to the LED light source device, and adjusting the pulsed voltage can achieve dimming of the LED light source device. Compared with the background technology, the PWM dimming device of the embodiment of the present application dims the LED light source device by quickly controlling the digital signal of the PWM controller, with higher adjustment frequency and accuracy and better reliability. At the same time, the power of the voltage source can also be relatively large, enabling high-power dimming. In addition, the cost of the voltage source is relatively low. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The drawings described herein are used to provide a further understanding of the present application and form a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation of the present application. In the drawings:

[0016] Figure 1 is a schematic diagram of the traditional analog voltage regulation method in the background technology;

[0017] Figure 2 is a schematic diagram of a PWM dimming device according to an embodiment of the present application;

[0018] Figure 3 is Figure 2 a schematic diagram of the pulsed voltage output by the PWM controller of the PWM dimming device shown;

[0019] Figure 4 is Figure 2 a schematic diagram of the LED light source device of the PWM dimming device shown;

[0020] Figure 5 is Figure 4 a schematic diagram of the fixing of the LED light source module and the fixing plate of the LED light source device shown;

[0021] Figure 6 is Figure 4 a schematic diagram of the geometric relationship of the LED light source device shown.

[0022] DESCRIPTION OF THE REFERENCE NUMERALS:

[0023] In the background technology:

[0024] 10 voltage source, 20 adjustable resistor, 30 LED light source device;

[0025] In the embodiment of the present application:

[0026] 100 LED light source device, 210 PWM controller, 220 voltage source;

[0027] 100-1 convex lens, 110 front arc surface of the convex lens, 120 center of the sphere of the front arc surface of the convex lens,

[0028] 130 principal optical axis of the convex lens,

[0029] 140 LED light source module, 141 lamp beads, 142 substrate, 150 fixing plate. Detailed implementation manners

[0030] In order to make the technical solutions and advantages in the embodiments of the present application clearer and more understandable, the following further describes the exemplary embodiments of the present application in detail with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than an exhaustive list of all embodiments. It should be noted that, without conflict, the embodiments in the present application and the features in the embodiments can be combined with each other.

[0031] The fluorescence-stained cell scanning and analysis system, abbreviated as the CTC scanning and analysis system, is a system for performing 360-degree image scanning and recognition on stained cells attached to a needle-shaped carrier. The fluorescence-stained cell scanning and analysis system includes multiple hardware devices, and the software is equipped with analysis software. The LED light source device in Embodiment 1 below is the PWM dimming device of the fluorescence-stained cell scanning and analysis system, and the fluorescence microscopy optical system in Embodiment 2 is the fluorescence microscopy optical system of the fluorescence-stained cell scanning and analysis system.

[0032] Embodiment 1

[0033] Figure 2 It is a schematic diagram of a PWM dimming device according to an embodiment of the present application; Figure 3 is Figure 2 A schematic diagram of the pulsed voltage output by the PWM controller of the shown PWM dimming device.

[0034] As Figure 2 and Figure 3 shown, a PWM dimming device according to an embodiment of the present application, for a fluorescence-stained cell scanning and analysis system, includes:

[0035] Voltage source 220;

[0036] PWM controller 210, configured to control the on / off of the voltage source to output a pulsed voltage;

[0037] LED light source device 100, the pulsed voltage is applied to the LED light source device.

[0038] The PWM dimming device according to the embodiment of the present application controls the on / off of a voltage source through a PWM controller to form a pulsed voltage and output the pulsed voltage. That is, the PWM controller can control the pulsed voltage applied to the LED light source device, and adjusting the pulsed voltage can achieve dimming of the LED light source device. Compared with the background art, the dimming of the LED light source device by the PWM dimming device according to the embodiment of the present application is achieved by quickly controlling the digital signal of the PWM controller, with higher adjustment frequency and accuracy, and better reliability. At the same time, the power of the voltage source can also be relatively large, enabling high-power dimming. In addition, the cost of the voltage source is relatively low.

[0039] During implementation, as Figure 3 shown, the PWM controller controls the pulse width and pulse frequency of the pulsed voltage to adjust the average brightness of the LED light source device.

[0040] The PWM controller can control the pulse width and pulse frequency of the pulsed voltage, thereby achieving adjustment of the average brightness of the LED light source device.

[0041] During implementation, the voltage source is a constant-voltage source. The power supply voltage output by the constant-voltage source is fixed. In this way, the voltage of the pulsed voltage is also fixed, and the voltage of the pulsed voltage is not adjusted. To adjust the average brightness of the LED light source device, only the pulse width and pulse frequency need to be adjusted.

[0042] During implementation, as Figure 2 shown, the voltage source 220, the PWM controller 210, and the LED light source device 100 are connected in series in sequence.

[0043] Such a sequential connection can enable the PWM controller to control the on / off of the voltage source to output a pulsed voltage, and the pulsed voltage is applied to the LED light source device.

[0044] During implementation, the average brightness of the LED light source device satisfies the following relational expression:

[0045]

[0046] where, E L is the average brightness of the LED light source device,

[0047] V is the voltage of the voltage source, R0 is the equivalent resistance of the voltage source,

[0048] R1 is the equivalent resistance of the LED light source device,

[0049] f is the pulse frequency of the pulsed voltage, τ is the pulse width of the pulsed voltage,

[0050] η is the electro-optical conversion efficiency of the LED light source device.

[0051] ΔT is the observation time. When the PWM dimming device is used as the dimming device of the fluorescence microscopy optical system, ΔT is less than the minimum exposure time of the fluorescence camera of the fluorescence microscopy optical system.

[0052] The derivation process is as follows:

[0053] The total work W done by the current of the LED light source device, where a part is the part E of the current work converted into light L , and the other part is the part E of the current work converted into heat. W = E L + E. The electro-optical conversion efficiency of the LED light source device is η. Therefore, it can be deduced that

[0054] Furthermore, it is deduced that Furthermore, by eliminating ΔT, it is finally deduced that

[0055] In implementation, the pulse frequency of the pulse voltage satisfies the following relationship:

[0056] f × ΔT > 100.

[0057] The pulse frequency of the pulse voltage that conforms to the above relationship can ensure the uniformity of the brightness of the LED light source device.

[0058] In implementation, the pulse width of the pulse voltage satisfies the following relationship:;

[0059]

[0060] where ε is the minimum sensitivity of the fluorescence camera of the fluorescence microscopy optical system; that is, the average brightness of the LED light source device is greater than the minimum sensitivity of the fluorescence camera of the fluorescence microscopy optical system, and the fluorescence camera can sense the light emitted by the LED light source device.

[0061] Next, the specific structure of the LED light source device 100 will be described.

[0062] Figure 4 is Figure 2 a schematic diagram of the LED light source device of the shown PWM dimming device; Figure 5 is Figure 4 a schematic diagram of the fixation of the LED light source module of the shown LED light source device to the fixing plate.

[0063] As Figure 4 and Figure 5As shown, the LED light source device 100 includes:

[0064] A convex lens 100-1, the front arc surface 110 of the convex lens being a spherical surface;

[0065] At least two LED light source modules 140, the LED light source modules 140 being disposed opposite to the front arc surface 110 of the convex lens, and the centers of the lamp beads 141 of each of the LED light source modules respectively facing the center of the sphere 120 of the front arc surface of the convex lens;

[0066] Wherein, the light emitted by the LED light source module 140 converges towards the center of the sphere 120 of the front arc surface of the convex lens through the convex lens 100-1.

[0067] The LED light source module is disposed opposite to the front arc surface of the convex lens, and the centers of the lamp beads of each of the LED light source modules respectively face the center of the sphere of the front arc surface of the convex lens. For the LED light source device, firstly, the number of LED light source modules is larger. Secondly, there are restrictions on the positions of the LED light source modules. The centers of the lamp beads of each of the LED light source modules respectively face the center of the sphere of the front arc surface of the convex lens. In this way, the light emitted by the lamp beads of each of the LED light source modules converges towards the center of the sphere of the front arc surface of the convex lens, so that the brightness around the center of the sphere of the front arc surface of the convex lens is relatively high. At the same time, since the position around the center of the sphere of the front arc surface of the convex lens is the position where the light of each of the LED light source modules interacts and compensates, therefore, the uniformity of the light spot around the center of the sphere of the front arc surface of the convex lens is relatively high.

[0068] In implementation, as Figure 4 shown, the lamp beads of one of the LED light source modules are located at the focal point of the convex lens, which is the focal point LED light source module;

[0069] The center of the sphere 120 of the front arc surface of the convex lens is located on the principal optical axis 130 of the convex lens.

[0070] The focal point LED light source module is located at the focal point of the convex lens, and the convex lens has a good converging effect on the light of the focal point LED light source module, so that the light intensity around the center of the sphere of the front arc surface of the convex lens is relatively high.

[0071] In implementation, as Figure 4 shown, the LED light source modules other than the focal point LED light source module are side LED light source modules;

[0072] The lamp beads of the side LED light source module are inclined towards the principal optical axis direction of the convex lens to achieve that the centers of the lamp beads of the side LED light source module face the center of the sphere of the front arc surface of the convex lens.

[0073] By adopting the above structure, it is possible to conveniently realize that the center of the lamp bead of the side LED light source module is oriented toward the center of the front arc surface of the convex lens.

[0074] In implementation, such as Figure 4 As shown, the projection of the lamp beads of the side LED light source module on the main optical axis of the convex lens is located between the lamp beads of the focus LED light source module and the convex lens.

[0075] That is, the object distance of the lamp beads of the side LED light source module is smaller than the focal length of the convex lens, and the light spot formed by the lamp beads of the side LED light source module around the center of the front arc surface of the convex lens and the light spot formed by the lamp beads of the focus LED light source module around the center of the front arc surface of the convex lens are well compensated for by the staggered compensation, and the light spot intensity around the center of the front arc surface of the convex lens is higher and the uniformity is also higher.

[0076] In implementation, the number of the side LED light source modules is n, where n is an integer greater than or equal to 2;

[0077] The n side LED light source modules are evenly distributed on the circumference of the same circle with the focus LED light source module as the center.

[0078] The side LED light source modules are evenly distributed on the circumference of the same circle with the focal LED light source module as the center, so the light spot formed around the center of the front arc surface of the convex lens is also roughly circular.

[0079] In implementation, as an optional implementation, Figure 4 and Figure 5 As shown, there are two side LED light source modules;

[0080] The two side LED light source modules are symmetrically arranged relative to the focal LED light source module.

[0081] The focal LED light source module and the two side LED light source modules are arranged in a line, so the light spot formed around the spherical center of the front arc surface of the convex lens is also roughly in a line.

[0082] In implementation, such as Figure 4 and Figure 5 As shown, there is a gap between the focus LED light source module and the side LED light source module.

[0083] The LED light source modules arranged at intervals are beneficial to the heat dissipation of the LED light source modules.

[0084] In implementation, such as Figure 4 As shown, the LED light source module further includes a fixing plate 150 for fixing the LED light source module 140;

[0085] The fixed plate 150 is disposed opposite to the front arc surface 110 of the convex lens, and the focal point LED light source module is fixed at the center position of the inner plate surface of the fixed plate.

[0086] The fixed plate realizes the fixation of multiple LED light source modules. The focal point LED light source module is fixed at the center position of the inner plate surface of the fixed plate, and the set position rule is convenient for processing and manufacturing.

[0087] During implementation, as Figure 4 shown, the edge position of the inner plate surface of the fixed plate is inclined towards the principal optical axis 130 of the convex lens;

[0088] The side LED light source module is fixed at the edge position of the inner plate surface of the fixed plate to enable the lamp beads of the side LED light source module to be inclined towards the principal optical axis 130 of the convex lens;

[0089] Among them, the fixed plate and the LED light source module form an LED light source assembly.

[0090] By inclining the edge position of the inner plate surface of the fixed plate towards the principal optical axis of the convex lens, the lamp beads of the side LED light source module are inclined towards the principal optical axis of the convex lens, and the structure is simple and easy to implement.

[0091] During implementation, as Figure 5 shown, the LED light source module 140 includes a square substrate 142 and a lamp bead 141 fixed at the center position of the substrate; the substrate can be a square as Figure 5 shown, or other shapes such as a circle, a rectangle, etc.;

[0092] The substrate is fixed to the fixed plate to realize the fixation of the LED light source module and the fixed plate.

[0093] During implementation, the following relationship is satisfied between the LED light source module and the convex lens:

[0094]

[0095] Among them, b is the distance between the projection of the center of the lamp bead of the side LED light source module and the projection of the center of the lamp bead of the focal point LED light source module in the direction perpendicular to the principal optical axis of the convex lens;

[0096] φ is the diameter of the convex lens, D is the focal length of the convex lens,

[0097] L is the side length of the substrate of the LED light source module,

[0098] θ is the included angle of the inclination of the side LED light source module relative to the principal optical axis direction of the convex lens,

[0099] α is the angle between the center of the lamp bead of the side LED light source module and the same-side edge of the convex lens.

[0100] The derivation process is as follows:

[0101] The angle of inclination of the edge position of the inner plate surface of the fixing plate towards the principal optical axis of the convex lens is equal to the angle of inclination of the side LED light source module relative to the principal optical axis of the convex lens, which is also θ. As Figure 6 shown, in △ABC, according to geometric relations, ∠BAC = α - θ; then

[0102] Since Substitute BC and AB into The following can be deduced

[0103] In implementation, b also satisfies the following relational expression:

[0104]

[0105] The derivation process is as follows:

[0106] As Figure 6 shown, according to geometric relations, Since The following can be deduced

[0107] In implementation, θ also satisfies the following relational expression:

[0108]

[0109] where r is the radius of the sphere where the front arc surface of the convex lens is located.

[0110] In implementation, θ and b should take the minimum value under the condition of satisfying the relational expressions and

[0111] Embodiment 2

[0112] The embodiment of the present application provides a fluorescence microscopy optical system for a fluorescence staining cell scanning and analysis system, including the PWM dimming device described in Embodiment 1.

[0113] Embodiment 3

[0114] A fluorescence staining cell scanning and analysis system according to an embodiment of the present application includes the PWM dimming device described in Embodiment 1.

[0115] Embodiment 4 ​

[0116] A fluorescence staining cell scanning and analysis system according to an embodiment of the present application includes the fluorescence microscopic optical system described in Embodiment 2.

[0117] In the description of the present application and its embodiments, it should be understood that the orientation or positional relationship indicated by terms such as "top", "bottom", "height", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present application.

[0118] In the present application and its embodiments, unless otherwise clearly defined and limited, terms such as "set", "installed", "connected", "coupled", "fixed", etc. should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection, an electrical connection, or a communication connection; it can be directly connected, or indirectly connected through an intermediate medium, and can be the internal communication of two elements or the interaction relationship between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to specific circumstances.

[0119] In the present application and its embodiments, unless otherwise clearly defined and limited, the first feature being "on" or "under" the second feature may include the first and second features being in direct contact, or may include the first and second features not being in direct contact but being in contact through other features therebetween. Moreover, the first feature being "above", "over" and "on" the second feature includes the first feature being directly above and obliquely above the second feature, or merely indicating that the first feature has a higher horizontal height than the second feature. The first feature being "under", "beneath" and "under" the second feature includes the first feature being directly below and obliquely below the second feature, or merely indicating that the first feature has a lower horizontal height than the second feature.

[0120] The above disclosure provides many different embodiments or examples for implementing different structures of the present application. In order to simplify the disclosure of the present application, the components and settings of specific examples are described above. Of course, they are only examples and are not intended to limit the present application. In addition, the present application may repeat reference numerals and / or reference letters in different examples. Such repetition is for the purpose of simplification and clarity, and does not itself indicate the relationship between the various embodiments and / or settings discussed. In addition, the present application provides examples of various specific processes and materials, but those of ordinary skill in the art may be aware of the application of other processes and / or the use of other materials.

[0121] Although the preferred embodiments of the present application have been described, additional changes and modifications can be made to these embodiments by those skilled in the art once they learn the basic creative concepts. Therefore, the appended claims are intended to be interpreted to include the preferred embodiments as well as all changes and modifications that fall within the scope of the present application.

[0122] Obviously, those skilled in the art can make various changes and modifications to the present application without departing from the spirit and scope of the present application. Thus, if these modifications and variations of the present application fall within the scope of the claims of the present application and their equivalent technologies, the present application is also intended to include these modifications and variations.

Claims

1. A PWM dimming device for a fluorescence-stained cell scanning and analysis system, characterized in that, Comprising: A voltage source; A PWM controller for controlling the on / off of the voltage source to output a pulsed voltage; An LED light source device to which the pulsed voltage is applied; The LED light source device includes: A convex lens, the front arc surface of the convex lens being spherical; At least two LED light source modules, the LED light source modules being disposed opposite to the front arc surface of the convex lens, and the centers of the lamp beads of each LED light source module respectively facing the center of the front arc surface of the convex lens; Wherein, the light emitted by the LED light source module converges towards the center of the front arc surface of the convex lens through the convex lens; The lamp beads of one of the LED light source modules are located at the focal point of the convex lens, being the focal point LED light source module; The center of the front arc surface of the convex lens is located on the principal optical axis of the convex lens; The LED light source modules other than the focal point LED light source module are side LED light source modules; The lamp beads of the side LED light source module are inclined towards the principal optical axis of the convex lens to enable the centers of the lamp beads of the side LED light source module to face the center of the front arc surface of the convex lens; The projection of the lamp beads of the side LED light source module on the principal optical axis of the convex lens is located between the lamp beads of the focal point LED light source module and the convex lens; The LED light source module includes a square substrate and a lamp bead fixed at the center position of the substrate; The PWM dimming device serves as the dimming device of the fluorescence microscopy optical system, and the average brightness of the LED light source device during the observation time satisfies the following relationship: where E L is the average brightness of the LED light source device, V is the voltage of the voltage source, R0 is the equivalent resistance of the voltage source, R1 is the equivalent resistance of the LED light source device, f is the pulse frequency of the pulsed voltage, τ is the pulse width of the pulsed voltage, η is the electro-optical conversion efficiency of the LED light source device, ΔT is the observation time, and ΔT is less than the minimum exposure time of the fluorescence camera of the fluorescence microscopy optical system; The pulse frequency of the pulsed voltage satisfies the following relationship: f×ΔT>100; The side LED light source modules are two; The two side LED light source modules are symmetrically disposed with respect to the focal point LED light source module.

2. The PWM dimming device according to claim 1, wherein, The PWM controller controls the pulse width and pulse frequency of the pulsed voltage to adjust the average brightness of the LED light source device.

3. The PWM dimming device according to claim 2, wherein, The voltage source is a constant voltage source.

4. The PWM dimming device according to claim 3, wherein The voltage source, the PWM controller and the LED light source device are connected in series in sequence.

5. The PWM dimming device according to claim 4, wherein The pulse width of the pulsed voltage satisfies the following relationship: Wherein, ε is the minimum sensitivity of the fluorescence camera of the fluorescence microscopy optical system.

6. The PWM dimming device according to claim 5, characterized in that, There is a gap between the focal point LED light source module and the side LED light source module.

7. The PWM dimming device according to claim 6, wherein It further includes a fixing plate for fixing the LED light source module; The fixing plate is disposed opposite to the front arc surface of the convex lens, and the focal point LED light source module is fixed at the center position of the inner plate surface of the fixing plate.

8. The PWM dimming device according to claim 7, wherein, The edge position of the inner plate surface of the fixing plate is inclined towards the principal optical axis of the convex lens; The side LED light source module is fixed at the edge position of the inner plate surface of the fixed plate, so as to make the lamp beads of the side LED light source module incline towards the principal optical axis direction of the convex lens.

9. The PWM dimming device according to claim 8, wherein The substrate is fixed to the fixed plate to realize the fixation of the LED light source module and the fixed plate.

10. The PWM dimming device according to claim 9, wherein, The following relationship is satisfied between the LED light source module and the convex lens: wherein, b is the distance between the projection of the center of the lamp bead of the side LED light source module and the projection of the center of the lamp bead of the focal point LED light source module in the direction perpendicular to the principal optical axis of the convex lens; φ is the diameter of the convex lens, D is the focal length of the convex lens, L is the side length of the substrate of the LED light source module, θ is the included angle of inclination of the side LED light source module relative to the principal optical axis direction of the convex lens, α is the included angle between the center of the lamp bead of the side LED light source module and the edge on the same side of the convex lens.

11. A fluorescence microscopy optical system for a fluorescence-stained cell scanning and analysis system, characterized in that, It includes the PWM dimming device according to any one of claims 1 to 10 above.

12. A fluorescence-stained cell scanning and analysis system, characterized in that, It includes the PWM dimming device according to any one of claims 1 to 10.

13. A fluorescence-stained cell scanning and analysis system, characterized in that, It includes the fluorescence microscopic optical system according to claim 11.

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