A water tank system for ornamental fish breeding and coral reef cultivation and its establishment method

Abstract

The present invention provides a water tank system for breeding ornamental fish and coral reefs and a method for establishing the same. The coral reef system comprises a breeding tank (1), a purified water pipeline (12), a gas pipeline (13), a filter element (14), a water quality filter box (2), an artificial light source (3) and a pumping system (4). The breeding tank (1) is connected to the water quality filter box (2) and the pumping system (4), and the water quality filter box (2) is connected to the pumping system (4). A gravel layer is laid in the breeding tank (1), and a trapezoidal frame is placed on the gravel layer. One side of the trapezoidal frame is placed on the gravel layer, and the upper and lower sides are grid hollow structures. The filter element comprises activated carbon, zeolite and phosphate rock powder. The corals obtained by the invention have good growth conditions, with bud length of 38.62-39.27 mm, bud height of 37.02-38.45 mm, and mass of 43.6-45.0 g. A water tank system suitable for ornamental fish breeding and coral reef cultivation is established, which can achieve a breeding environment close to nature, enable the corals to reproduce and grow normally, and achieve good growth quality.

Description

Technical Field

[0001] The invention relates to the field of coral cultivation, and in particular to a water tank system for ornamental fish breeding and coral reef cultivation and a method for establishing the system. Background Art

[0002] Coral reefs are the most important ecosystems in the ocean, and research on coral reefs has extremely high scientific value. However, due to the uncontrollability and uncertainty of the marine environment, the relevant research on corals is greatly limited. Seeking a controllable and highly restored artificial coral reef system is also an important research direction at present.

[0003] One way to solve the problem of insufficient natural drug sources is to expand the source of marine drugs by artificially breeding lower marine animals, isolating and obtaining drug lead compounds from them, and expanding the source of marine drugs.

[0004] Due to the high cost of maintaining water quality and high technical requirements, in domestic aquariums, coral reef landscapes are basically achieved through fake corals, such as coral models made of plastic or resin. The only living corals can only be displayed in very small aquariums, and the species are extremely limited. The main reason is that corals have very strict requirements on water quality. On Earth, only 3% of the sea area is suitable for coral survival. Therefore, the fundamental problem of ensuring the survival of corals in an artificial environment is the control of water quality. Therefore, providing a water tank system suitable for fish breeding and coral reef cultivation is one of the effective means to solve the problem of coral cultivation. Summary of the invention

[0005] In view of this, the object of the present invention is to provide a water tank system for ornamental fish breeding and coral reef cultivation and a method for establishing the system to solve the above problems.

[0006] The technical solution of the present invention is achieved in this way:

[0007] A water tank system for breeding ornamental fish and cultivating coral reefs, the coral reef system comprising a cultivation tank (1), a purified water pipeline (12), an air transmission pipeline (13), a filter element (14), a water quality filter box (2), an artificial light source (3) and a pumping system (4), wherein the cultivation tank (1) and the water quality filter box (2) are connected to each other via the pumping system (4);

[0008] A gravel layer is laid in the breeding box (1), and a trapezoidal frame is placed on the gravel layer, one side of the trapezoidal frame is placed on the gravel layer, and the upper and lower sides are grid hollow structures; the upper side of the trapezoid is about 2 cm high from the gravel layer, and the lower side of the trapezoid is about 4 cm high from the gravel layer, and a wave pump is used to simulate the flow of natural water bodies; the filter element includes activated carbon, zeolite and phosphate rock powder.

[0009] It is further described that in the water quality filter box (2), nitrifying bacteria particles are placed in the water quality filter box (2) for cultivation to form a nitrifying microenvironment; and the mass ratio of the activated carbon, zeolite and phosphate rock powder is 1-2:1-2:1-2.

[0010] It is further explained that the preparation method of the nitrifying bacteria granules is: bloodstone and air stone are selected, stirred and mixed, granulated, roasted, cooled, sodium carboxymethyl cellulose and nitrifying bacteria powder are added, stirred, ball-milled, and nitrifying bacteria granules are obtained.

[0011] It is further described that the mass ratio of the bloodstone, air stone, sodium carboxymethyl cellulose and nitrifying bacteria powder is 1:1-2:0.8-0.9:0.2-0.4, the roasting temperature is 280-320°C, the roasting time is 2-3h, and the ball milling time is 4-5h.

[0012] To further explain, the culture is as follows: nitrifying bacteria particles are added at a dosage of 15-25 g / m3, and cultured at 25-28°C for 20-24 hours, and then nitrifying bacteria particles are added at a dosage of 10-12 g / m3, and cultured at 25-28°C for 6-8 hours.

[0013] The present invention provides a method for establishing a water tank system for ornamental fish breeding and coral reef cultivation, comprising the following steps:

[0014] S1: Place filtered seawater in the culture tank (1) and check the water quality regularly;

[0015] S2: Establish the nitrification microenvironment of the water filtration box;

[0016] S3: After the system environment is stable, corals are added and light and water quality management is performed;

[0017] S4: After 30-35 days of coral cultivation, fish fry are added and light and water quality management are carried out.

[0018] To further illustrate, in step S3, the light intensity is 6000-8000K, and in step S4, the light intensity is 4000-5000K.

[0019] To further illustrate, when the illumination intensity is 6000-8000K, the light sources are set to white light and blue light, and when the illumination intensity is 4000-5000K, the light sources are set to white light, blue light and yellow light.

[0020] To further illustrate, when the illumination intensity is 6000-8000K, the ratio of white light to blue light is set to 2:2-3, and when the illumination intensity is 4000-5000K, the ratio of white light, blue light and yellow light is set to 2:2:2-3.

[0021] Further explanation: water quality conditions are: salinity 0.022-0.025, pH 8.0-8.2, temperature 25-28°C, dissolved oxygen greater than 3.2 mg / L, and ammonia nitrogen less than 0.2 mg / L.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The present invention establishes a coral reef water tank culture mode through a culture box, a purified water pipeline, a gas pipeline, a filter element, a water quality filter box, an artificial light source and a pumping system, and lays a gravel layer in the culture box, and places a trapezoidal rack on the gravel layer, so as to achieve a culture environment close to nature, so that the corals can reproduce and grow normally, in order to provide theoretical support and data reference for large-scale medicinal coral culture.

[0024] The coral reef water tank culture mode established by the present invention can help corals quickly adapt to artificial culture, improve the feeding rate of corals, and help to obtain corals with higher growth performance. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 Schematic diagram of the water tank system for breeding ornamental fish and coral reefs according to Example 1 of the present invention, wherein 1 is a breeding tank, 2 is a water quality filter box, 3 is an artificial light source, 4 is a pumping system, 12 is a purified water pipeline, 13 is an air pipeline, 14 is a filter element, 15 is a gravel layer, 16 is a trapezoidal rack, and 21 is nitrifying bacteria particles. DETAILED DESCRIPTION

[0026] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0027] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0028] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0029] Example 1 - A water tank system for ornamental fish breeding and coral reef cultivation

[0030] The water tank system includes a breeding tank 1, a purified water pipeline 12, a gas pipeline 13, a filter element 14, a water filter box 2, an artificial light source 3 and a pumping system 4. The breeding tank 1 is connected to the water filter box 2 and the pumping system 4, and the water filter box 2 is connected to the pumping system 4.

[0031] A gravel layer is laid in the breeding box 1, and a trapezoidal frame is placed on the gravel layer, one side of the trapezoidal frame is placed on the gravel layer, and the upper and lower sides are grid hollow structures; the upper side of the trapezoid is about 2 cm high from the gravel layer, and the lower side of the trapezoid is about 4 cm high from the gravel layer. Water is input through a pumping system to simulate the flow of natural water bodies to form an artificial wave effect, and a filter element is placed inside the trapezoidal frame;

[0032] The filter element is composed of activated carbon, zeolite and phosphate rock powder in a mass ratio of 1:1:1.

[0033] Example 2 - Construction of a water tank system for ornamental fish breeding and coral reef cultivation

[0034] S1: Put filtered seawater into the aquaculture box 1 of Example 1 and check the water quality regularly;

[0035] S2: Establish the nitrification microenvironment of water quality filter box 2: with a dosage of 15g / m 3 , put nitrifying bacteria particles into the water filter box 2, incubate at 25-28℃ for 20h, and then add 10g / m 3 , add nitrifying bacteria particles, culture at 25-28℃ for 6h,

[0036] The preparation method of the nitrifying bacteria granules is as follows: bloodstone and air stone are selected in a mass ratio of 1:1:0.8:0.2, stirred and mixed, granulated, roasted at 280°C for 2h, cooled, added with sodium carboxymethyl cellulose and nitrifying bacteria powder, stirred, and ball-milled for 4h to obtain nitrifying bacteria granules.

[0037] S3: After the system environment is stable, corals are added and the light source ratio is set to 2:3 for white light and blue light for light cultivation. The light intensity is 6000K.

[0038] S4: After 30 days of coral culture, fish fry were added and the light source ratio of white light, blue light and yellow light was set to 2:2:3 for light culture, and the light intensity was 4000K;

[0039] The water quality is tested regularly. During the breeding process, the water quality conditions are controlled as follows: salinity 0.022-0.025, pH 8.0-8.2, temperature 25-28°C, dissolved oxygen greater than 3.2 mg / L, ammonia nitrogen less than 0.2 mg / L, and adjustments and management are carried out within this range.

[0040] Example 3 - Construction of a water tank system for ornamental fish breeding and coral reef cultivation

[0041] S1: Put filtered seawater into the aquaculture box 1 of Example 1 and check the water quality regularly;

[0042] S2: Establish the nitrification microenvironment of water quality filter box 2: with a dosage of 25g / m 3, put nitrifying bacteria particles into the water filter box 2, incubate at 25-28℃ for 24h, and then add 12g / m 3 , add nitrifying bacteria particles, culture at 25-28℃ for 8h,

[0043] The preparation method of the nitrifying bacteria granules is as follows: bloodstone and air stone are selected in a mass ratio of 1:2:0.9:0.4, stirred and mixed, granulated, roasted at 320°C for 3h, cooled, added with sodium carboxymethyl cellulose and nitrifying bacteria powder, stirred, and ball-milled for 5h to obtain nitrifying bacteria granules.

[0044] S3: After the system environment is stable, corals are added and the light source ratio is set to 2:2 for white light and blue light for light cultivation. The light intensity is 8000K.

[0045] S4: After 35 days of coral culture, fish fry were added and the light source ratio of white light, blue light and yellow light was set to 2:2:2 for light culture, and the light intensity was 5000K;

[0046] The water quality is tested regularly. During the breeding process, the water quality conditions are controlled as follows: salinity 0.022-0.025, pH 8.0-8.2, temperature 25-28°C, dissolved oxygen greater than 3.2 mg / L, ammonia nitrogen less than 0.2 mg / L, and adjustments and management are carried out within this range.

[0047] Example 4 - Construction of a water tank system for ornamental fish breeding and coral reef cultivation

[0048] S1: Put filtered seawater into the aquaculture box 1 of Example 1 and check the water quality regularly;

[0049] S2: Establish the nitrification microenvironment of water quality filter box 2: with a dosage of 20g / m 3 , put nitrifying bacteria particles into the water filter box 2, incubate at 25-28℃ for 22h, and then add 15g / m 3 , add nitrifying bacteria particles and culture at 25-28℃ for 7h;

[0050] The preparation method of the nitrifying bacteria granules is as follows: bloodstone and air stone are selected in a mass ratio of 1:2:0.8:0.5, stirred and mixed, granulated, roasted at 300°C for 3h, cooled, added with sodium carboxymethyl cellulose and nitrifying bacteria powder, stirred, and ball-milled for 5h to obtain nitrifying bacteria granules.

[0051] S3: After the system environment is stable, corals are added and the light source ratio is set to 2:3 for white light and blue light for light cultivation. The light intensity is 7000K.

[0052] S4: After 35 days of coral culture, fish fry were added and the light source ratio of white light, blue light and yellow light was set to 2:2:3 for light culture, and the light intensity was 6000K;

[0053] The water quality is tested regularly. During the breeding process, the water quality conditions are controlled as follows: salinity 0.022-0.025, pH 8.0-8.2, temperature 25-28°C, dissolved oxygen greater than 3.2 mg / L, ammonia nitrogen less than 0.2 mg / L, and adjustments and management are carried out within this range.

[0054] Comparative Example 1 - Construction of a water tank system for ornamental fish breeding and coral reef cultivation

[0055] S1: The water tank system includes a breeding tank 1, a purified water pipeline 12, a gas pipeline 13, a filter element 14, and an artificial light source 3;

[0056] A gravel layer is laid in the breeding box 1, and a trapezoidal frame is placed on the gravel layer, one side of the trapezoidal frame is placed on the gravel layer, and the upper and lower sides are grid hollow structures; the upper side of the trapezoid is about 2 cm high from the gravel layer, and the lower side of the trapezoid is about 4 cm high from the gravel layer. Water is input through a pumping system to simulate the flow of natural water bodies to form an artificial wave effect, and a filter element is placed inside the trapezoidal frame;

[0057] The filter element is composed of activated carbon, zeolite and phosphate rock powder in a mass ratio of 1:1:1.

[0058] Place filtered seawater in the culture tank 1 and check the water quality regularly;

[0059] S2: Establish the nitrification microenvironment of water quality breeding box 1: put in 20g / m 3 , put nitrifying bacteria particles into the breeding box 1;

[0060] S3: After the system environment is stable, corals are added and the light source ratio is set to 2:3 for white light and blue light for light cultivation. The light intensity is 7000K.

[0061] After 10 days of coral culture, add 15g / m 3 , add nitrifying bacteria particles;

[0062] The preparation methods of the above-mentioned nitrifying bacteria granules of S2 and S3 are: bloodstone and air stone are selected in a mass ratio of 1:2:0.8:0.5, stirred and mixed, granulated, roasted at 300°C for 3h, after cooling, sodium carboxymethyl cellulose and nitrifying bacteria powder are added, stirred, and ball-milled for 5h to obtain nitrifying bacteria granules.

[0063] S4: After 35 days of coral culture, fish fry were added and the light source ratio of white light, blue light and yellow light was set to 2:2:3 for light culture, and the light intensity was 6000K;

[0064] The water quality is tested regularly. During the breeding process, the water quality conditions are controlled as follows: salinity 0.022-0.025, pH 8.0-8.2, temperature 25-28°C, dissolved oxygen greater than 3.2 mg / L, ammonia nitrogen less than 0.2 mg / L, and adjustments and management are carried out within this range.

[0065] The culture was carried out for 50 days. Rotifers were used for feeding during the culture process. The culture conditions of corals in each tank were counted. The net photosynthetic rate was determined as follows: after the culture was completed, corals from each tank were taken and placed in containers filled with culture water from the respective tanks. -2 ·s -1 Under light conditions, the net photosynthetic rate was measured using a BDO-821 dissolved oxygen meter.

[0066] Table 1 Coral culture in the tank system

[0067]

[0068]

[0069] As shown in Table 1 above, the growth of the corals in Example 2-4 was good, with a survival rate of 100%. The bud length was 38.62-39.27 mm, the bud height was 37.02-38.45 mm, the mass was 43.6-45.0 g, and the net photosynthetic rate was 0.296-0.325 μmol·cm -2 ·h -1 , indicating that the present invention establishes a coral reef water tank culture mode through a culture box, a purified water pipeline, a gas pipeline, a filter element, a water quality filter box, an artificial light source and a pumping system, and lays a gravel layer in the culture box, and places a trapezoidal rack on the gravel layer, so as to achieve a culture environment close to nature, so that the coral can reproduce and grow normally. The appropriate culture environment can help the coral quickly adapt to artificial culture and obtain higher growth performance. At the same time, the ornamental fish cultured in the water tank did not die. Compared with comparative example 1, directly putting nitrifying bacteria particles is not easy to make the environment of the culture water tank form a stable system, but makes the system change rapidly during the period of putting. Corals are a kind of "passive" feeding. The system changes in the short term are more sufficient to make the corals quickly feed to a saturated state, but once the stable stage is passed, it is very easy to reduce the "enthusiasm" of coral feeding, thereby affecting the healthy growth of corals.

[0070] Example 5 - Construction of a water tank system for ornamental fish breeding and coral reef cultivation

[0071] S1: Put filtered seawater into the aquaculture box 1 of Example 1 and check the water quality regularly;

[0072] S2: Establish the nitrification microenvironment of water quality filter box 2: with a dosage of 20g / m 3, put nitrifying bacteria particles into the water filter box 2, incubate at 25-28℃ for 22h, and then add 15g / m 3 , add nitrifying bacteria particles and culture at 25-28℃ for 7h;

[0073] The preparation method of the nitrifying bacteria granules is as follows: bloodstone and air stone are selected in a mass ratio of 1:2:0.8:0.5, stirred and mixed, granulated, roasted at 300°C for 3h, cooled, added with sodium carboxymethyl cellulose and nitrifying bacteria powder, stirred, and ball-milled for 5h to obtain nitrifying bacteria granules.

[0074] S3: After the system environment is stable, corals are added and cultured under white light with a light intensity of 7000K;

[0075] S4: After 35 days of coral culture, fish fry were added and the light source ratio was set to 2:3 for white light and blue light for light culture, and the light intensity was 6000K;

[0076] The water quality is tested regularly. During the breeding process, the water quality conditions are controlled as follows: salinity 0.022-0.025, pH 8.0-8.2, temperature 25-28°C, dissolved oxygen greater than 3.2 mg / L, ammonia nitrogen less than 0.2 mg / L, and adjustments and management are carried out within this range.

[0077] After testing, the coral of Example 5 had a mass of 41.1 g and a net photosynthetic rate of 0.271 μmol·cm -2 ·h -1 Studies have shown that suitable lighting promotes the growth of corals. The present invention finds that the net photosynthetic rate of corals can be increased by lighting with different light source ratios, which helps to provide corals with sufficient energy for feeding.

[0078] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A water tank system for ornamental fish breeding and coral reef cultivation, characterized in that: The water tank system comprises a breeding box (1), a purified water pipeline (12), a gas pipeline (13), a filter element (14), a water quality filter box (2), an artificial light source (3) and a pumping system (4); the breeding box (1) and the water quality filter box (2) are connected to each other via the pumping system (4); A gravel layer is laid in the breeding box (1), a trapezoidal frame is placed on the gravel layer, one side of the trapezoidal frame is placed on the gravel layer, the upper and lower sides are grid hollow structures, and a filter core (14) is placed inside the trapezoidal frame; the filter core (14) includes activated carbon, zeolite and phosphate rock powder; The method for establishing the water tank system for ornamental fish breeding and coral reef cultivation comprises the following steps: S1: Place filtered seawater in the culture tank (1) and check the water quality regularly; S2: establishing a nitrification microenvironment of the water quality filter box (2); in the water quality filter box (2), nitrifying bacteria particles are placed in the water quality filter box (2) for cultivation to form a nitrification microenvironment; the preparation method of the nitrifying bacteria particles is: selecting bloodstone and air stone, stirring and mixing, granulating, roasting, cooling, adding sodium carboxymethyl cellulose and nitrifying bacteria powder, stirring, and ball milling to obtain nitrifying bacteria particles; S3: After the system environment is stable, corals are added and light and water quality management is performed; S4: After 30-35 days of coral culture, fish fry are added and light and water quality management is carried out; In step S3, the illumination intensity is 6000-8000K, and the light sources are set to have a quantity ratio of white light to blue light of 2:2~3. In step S4, the illumination intensity is 4000-5000K, and the light sources are set to have a quantity ratio of white light, blue light and yellow light of 2:2:2~3.

2. The water tank system for ornamental fish breeding and coral reef cultivation according to claim 1 is characterized in that: The mass ratio of the activated carbon, zeolite and phosphate rock powder is 1-2:1-2:1-2.

3. The water tank system for ornamental fish breeding and coral reef cultivation according to claim 1 is characterized in that: The mass ratio of the bloodstone, air stone, sodium carboxymethyl cellulose and nitrifying bacteria powder is 1:1-2:0.8-0.9:0.2-0.4, the roasting temperature is 280-320°C, the roasting time is 2-3 hours, and the ball milling time is 4-5 hours.

4. The water tank system for ornamental fish breeding and coral reef cultivation according to claim 1 is characterized in that: The culture is: with a dosage of 15-25g / m 3 , add nitrifying bacteria particles, culture at 25-28℃ for 20-24h, and then add 10-12g / m 3 , add nitrifying bacteria particles and culture at 25-28℃ for 6-8h.

5. The water tank system for ornamental fish breeding and coral reef cultivation according to claim 1, characterized in that: Water quality conditions are: salinity 0.022-0.025, pH 8.0-8.2, temperature 25-28℃, dissolved oxygen greater than 3.2mg / L, and ammonia nitrogen less than 0.2mg / L.

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