A recombinant lactococcus lactis live vector vaccine and a preparation method and application thereof

By expressing the UreA gene, the urease subunit of Helicobacter pylori, in Lactococcus lactis, the problem of oral vaccine delivery and immunization efficacy in the gastrointestinal tract was solved, achieving efficient mucosal immune response and immune protection.

CN116966283BActive Publication Date: 2026-05-29WEST CHINA HOSPITAL SICHUAN UNIV
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Patent Information

Application Number
CN202311105452.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-08-30
Publication Date
2026-05-29
Estimated Expiration
2043-08-30

AI Technical Summary

Technical Problem

In existing technologies, oral Helicobacter pylori vaccines have weak immune effects, are difficult to function effectively in the gastrointestinal tract, and suffer from problems such as gastric acid corrosion and protease degradation, making it difficult to maintain antigen activity in the long term and making it difficult to select delivery vectors.

Method used

Using Lactococcus lactis as a live vector, the UreA gene, the urease subunit of Helicobacter pylori, was linked to a plasmid expression vector and electroporated into Lactococcus lactis, which expressed and secreted the UreA protein, stimulating the intestinal mucosal immune system to produce an immune response.

Benefits of technology

It achieved a highly efficient mucosal immune response. The oral lactococcal vaccine produced an immune protection rate of over 60% in mice and effectively inhibited the colonization of Helicobacter pylori.

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Abstract

The application belongs to the technical field of biological pharmacy, and discloses a recombinant lactococcus lactis live carrier vaccine, which is obtained by connecting a urease subunit UreA gene with a plasmid expression vector and then electrically transforming the lactococcus lactis; the amino acid sequence of the urease subunit UreA gene is shown as SEQ ID NO. 1. The application further discloses application of the recombinant lactococcus lactis live carrier vaccine in preparation of an anti-helicobacter pylori vaccine. The recombinant lactococcus lactis live carrier vaccine provided by the application can effectively stimulate the body to produce a mucosal immune response and has good protection effect.
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