Recombinant HVT co-expressing H9 subtype AIV HA gene, IBV S gene and IBDV VP2 gene and its application

By inserting the H9 subtype avian influenza virus HA gene, infectious bronchitis virus S gene and infectious bursitis VP2 gene into the turkey herpes virus (HVT) genome, a recombinant HVT virus strain that can jointly express these genes was constructed, solving the problem that traditional vaccines cannot effectively prevent and control H9 subtype avian influenza, chicken infectious bronchitis and chicken infectious bursitis, and achieving safe and efficient multiple vaccine protection effects.

CN119372158BActive Publication Date: 2025-04-04INST OF URBAN AGRI CHINESE ACADEMY OF AGRI SCI +1
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Patent Information

Application Number
CN202411920853.7
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-12-25
Publication Date
2025-04-04
Estimated Expiration
2044-12-25

AI Technical Summary

Technical Problem

Traditional inactivated vaccines can no longer effectively prevent and control the prevalence of H9 subtype avian influenza, chicken infectious bronchitis and chicken infectious bursal disease, and it is necessary to develop a safer and more efficient new vaccine.

Method used

By inserting the H9 subtype avian influenza virus HA gene, infectious bronchitis virus S gene and infectious bursal virus VP2 gene into the turkey herpes virus (HVT) genome, a recombinant HVT virus strain that can jointly express these genes was constructed for the preparation of vaccines.

Benefits of technology

The recombinant HVT vaccine has good replication ability and genetic stability in chickens, and can provide immune protection against H9 subtype avian influenza, QX type IBV poison and IBDV super poison, achieving the effect of preventing three viruses simultaneously.

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Abstract

The present invention discloses a recombinant HVT co-expressing H9 subtype AIV HA gene, IBV S gene and IBDV VP2 gene and its application, belonging to the technical field of medicine or veterinary medicine. The recombinant HVT is obtained by inserting an expression cassette containing H9 subtype AIV HA gene and QX type IBV S gene into the 95322-95323 nucleotides of HVT FC126 strain, and inserting an expression cassette containing IBDV classic strong strain VP2 gene into the 112071-112088 nucleotides of HVT genome, and then recombinant HVT vaccine strain H30201 co-expressing H9 subtype AIV HA gene, IBV S gene and IBDV VP2 gene is obtained by virus rescue. The present invention also proposes the application of the recombinant HVT vaccine strain in the preparation of H9 subtype avian influenza, chicken infectious bronchitis and chicken infectious bursal disease vaccines.
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