Catharanthus roseus ein3-eils transcription factor and application thereof
By cloning and overexpressing the EIN3/EILs transcription factor CrEIN3 from periwinkle, the expression of key enzyme genes was activated, solving the problem of low yield of terpenoid indole alkaloids in periwinkle and achieving efficient synthesis and increased yield of vincristine.
CN119570811BActive Publication Date: 2026-03-20SHANGHAI JIAOTONG UNIV
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Patent Information
- Application Number
- CN202411834070.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-12
- Publication Date
- 2026-03-20
- Estimated Expiration
- 2044-12-12
AI Technical Summary
Technical Problem
Existing technologies are insufficient to efficiently increase the yield of terpenoid indole alkaloids in periwinkle, resulting in high extraction costs and low industrial production efficiency, thus lacking an economical production method.
Method used
By cloning and overexpressing the EIN3/EILs transcription factor CrEIN3 from periwinkle, the expression of key enzyme genes DXS1, SGD, and PRX1 in the vincristine biosynthesis pathway was activated, thereby promoting vincristine biosynthesis.
Benefits of technology
It significantly increased the synthesis of vincristine in periwinkle, providing a new strategy for the genetic engineering breeding and large-scale production of periwinkle, and increasing the yield of vincristine.
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Abstract
The application discloses a Catharanthus roseus EIN3-EILs transcription factor and application thereof, relates to the field of biotechnology, and the nucleotide sequence of the transcription factor CrEIN3 is shown as SEQ ID NO:1, and the protein coding sequence is shown as SEQ ID NO:2. The overexpression method is as follows: cloning the Catharanthus roseus CrEIN3 gene; constructing an overexpression vector; obtaining an agrobacterium engineering strain through agrobacterium mediation; and transiently transforming the Catharanthus roseus petals. The Catharanthus roseus EIN3-EILs transcription factor CrEIN3 is applied to increasing the content of vinblastine in the Catharanthus roseus. Through transient overexpression of CrEIN3 in the Catharanthus roseus petals, the synthesis of vinblastine in the Catharanthus roseus petals can be remarkably improved, and this has important significance for efficient production of vinblastine in the Catharanthus roseus.
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