Biological attractant for pomacea canaliculata as well as preparation method and application of biological attractant

Abstract

The invention relates to the technical field of biological control, and discloses a pomacea canaliculata biological attractant and a preparation method and application thereof, and the pomacea canaliculata biological attractant comprises 100 parts of croton fruit powder, 100 parts of attapulgite, 3 parts of an adhesive, 700 parts of a phagostimulant and 100 parts of an auxiliary material. According to the pomacea canaliculata biological attractant disclosed by the invention, the pomacea canaliculata is dead due to poisoning and malnutrition after being fed by combining the dual effects of plant poisoning of croton fruits and physiological killing of attapulgite.

Description

Technical Field

[0001] The invention relates to the technical field of biological control, in particular to a biological attractant for Pomacea canaliculata and a preparation method and application thereof. Background Art

[0002] In recent years, due to climate change and improper agricultural water management, the golden apple snail (Pomacea canaliculata) has spread rapidly in rice fields and wetland environments, seriously threatening crop production. Golden apple snails feed on plant stems and leaves, causing plant damage, especially in rice-growing areas, where they are particularly destructive to crops. Currently, chemical agents used to control golden apple snails, such as metaldehyde and scutellaria, are effective but have environmental pollution problems and are prone to harm non-target organisms. The prior art lacks a preparation for controlling golden apple snails that is environmentally friendly, residue-free, and has little impact on non-target organisms.

[0003] In view of the deficiencies of the above-mentioned prior art solutions, the present invention provides a bio-attractant for Pomacea canaliculata and a preparation method and application thereof. Therefore, it is necessary to develop a bio-attractant for Pomacea canaliculata and a preparation method and application thereof. Summary of the invention

[0004] The purpose of the present invention is to provide a bio-attractant for Pomacea canaliculata and its preparation method and application, so as to solve the problems existing in the above-mentioned prior art, aiming to effectively control Pomacea canaliculata through innovative materials and preparation processes, while avoiding the environmental pollution problems caused by chemical agents. To achieve the above-mentioned purpose, the present invention is implemented according to the following technical scheme:

[0005] On the one hand, the present invention comprises the following ingredients: 100 parts of croton fruit powder, 100 parts of attapulgite, 3 parts of adhesive, 700 parts of attractant and 100 parts of auxiliary materials.

[0006] Furthermore, the adhesive is a sodium polyacrylate solution, and the sodium polyacrylate and water are prepared at a ratio of 1:333 to prepare a 0.003 g / mL sodium polyacrylate solution.

[0007] Furthermore, the appetite attractant includes yeast powder, cooked soybean powder, raw soybean powder, corn powder and soybean oil.

[0008] Furthermore, the attractant is composed of cooked soybean powder, raw soybean powder, yeast powder, corn powder and soybean oil in a compound mass ratio of 25:13:2:29:1.

[0009] Furthermore, the auxiliary material comprises wood cellulose, butylparaben and rice straw powder in a mass ratio of 1:1:2.

[0010] A method for preparing a Pomacea canaliculata biological attractant, used for preparing the Pomacea canaliculata biological attractant, characterized in that:

[0011] S1. Grind the croton fruit into 40-60 mesh to obtain croton fruit powder;

[0012] S2. Place the attractant, croton fruit powder and sodium polyacrylate aqueous solution in an extrusion granulator for granulation. The granulated particles have a length of 3.0 to 4.0 mm, a width of 2 to 3 mm, a moisture content of 40%, and are dried at below 50°C or in the sun.

[0013] The Pomacea canaliculata trapping agent is evenly spread in the irrigation canal of the rice seedling field, with the dosage of 2-3 kg per mu of the rice seedling field and 1 kg per m2 of the irrigation area. 2 Apply 60-70g and spread it every 10-20 days.

[0014] The beneficial effects of the present invention are:

[0015] The present invention discloses a bio-attractant for Pomacea canaliculata and a preparation method and application thereof. Compared with the prior art, the present invention has the following technical effects:

[0016] 1. The bio-attractant for Pomacea canaliculata of the present invention has a strong feeding effect on Pomacea canaliculata, allowing the Pomacea canaliculata to actively feed on the bio-attractant.

[0017] 2. The bio-attractant for Pomacea canaliculata of the present invention combines the dual effects of the phytotoxicity of croton fruit (toxicity of croton to Pomacea canaliculata) and the physiological killing (intestinal obstruction) of attapulgite, thereby causing the Pomacea canaliculata to die of poisoning and malnutrition after feeding on the Pomacea canaliculata.

[0018] 3. The bio-attractant for golden apple snails of the present invention is environmentally friendly and free of pesticide residues, and has no risk of excessive rice pesticide residues and soil non-point source pollution. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a comparison diagram of indoor experiments of an implementation case of a Pomacea canaliculata biological attractant and a preparation method thereof of the present invention;

[0020] Figure 2 It is a comparison diagram of indoor experiments of an implementation case of a Pomacea canaliculata biological attractant and a preparation method thereof of the present invention;

[0021] Figure 3 A field experiment comparison diagram of an implementation case of a bio-attractant for Pomacea canaliculata and a preparation method thereof according to the present invention;

[0022] Figure 4 This is a field experiment comparison diagram of an implementation case of a bio-attractant for Pomacea canaliculata and a preparation method thereof according to the present invention. DETAILED DESCRIPTION

[0023] The present invention is further described below by means of specific embodiments. The illustrative embodiments and descriptions of the present invention are used to explain the present invention but are not intended to limit the present invention.

[0024] Example 1 - Indoor toxicity test of preparations with different ratios on Pomacea canaliculata

[0025] Experimental Materials:

[0026] Formula 1: 100g of croton fruit powder, 100g of attapulgite, 700g of attractant, and 100g of auxiliary materials;

[0027] Formula 2: croton powder, attapulgite, sodium polyacrylate, cooked soybean powder, raw soybean powder, corn powder, yeast powder, soybean oil, wood cellulose, butyl paraben, and rice straw powder.

[0028] Formula 3: croton powder, attapulgite, sodium polyacrylate, cooked soybean powder, raw soybean powder, corn powder, yeast powder, soybean oil, wood cellulose, and butylparaben.

[0029] Formula 4: croton powder, sodium polyacrylate, cooked soybean powder, raw soybean powder, corn powder, yeast powder, soybean oil, wood cellulose, butyl paraben, and rice straw powder.

[0030] Formula 5: croton powder, attapulgite, sodium polyacrylate, corn flour, wood cellulose, butyl paraben, and rice straw powder.

[0031] Formula 6: croton powder, attapulgite, sodium polyacrylate, wood cellulose, butyl paraben, and rice straw powder.

[0032] Positive control: an existing chemical control agent for Pomacea canaliculata (metaldehyde granules)

[0033] Blank control: A blank control without any pesticide, only feeding the same amount of rice seedlings.

[0034] See Table 1 for specific ingredient contents

[0035] Table 1 Test formula of bio-attractant for Pomacea canaliculata

[0036]

[0037] Example 1 - Laboratory trapping experiment of Pomacea canaliculata with different ratios of Pomacea canaliculata biological trapping agents

[0038] Test apple snails: Pomacea canaliculata, collected from pollution-free rice fields.

[0039] Experimental method: Pomacea canaliculata was cultured in a 50cm×40cm×30cm polypropylene (PP) plastic box with a water depth of 3.5cm. Ten healthy Pomacea canaliculata of the same size (body length of about 2.5cm) were placed in each box. Five days before the start of the experiment, the Pomacea canaliculata was starved for 4 days to empty the intestines. Five replicates were set for each treatment. 0.9g of Pomacea canaliculata biological attractant was put into each box of the treatment group, 0.15g of tetraacetaldehyde was put into the positive control according to the pesticide instructions, and 0.9g of rice seedlings was put into each box of the blank control. After the application of the pesticide, the number of Pomacea canaliculata dead was observed and recorded every day, and the dead Pomacea canaliculata was removed in time to prevent the water quality from being affected. The total observation and statistics were 4 days, and the mortality rate of Pomacea canaliculata was calculated for 4 days. The control effect was judged according to the difference between the mortality rate of the control group and the mortality rate of the treatment group. The calculation results are shown in Table 2.

[0040] Data processing:

[0041] Mortality rate = number of deaths / total number × 100%

[0042] Adjusted mortality rate = (mortality rate of experimental group - mortality rate of control group) / (1 - mortality rate of control group) × 100% Table 2 Mortality of Pomacea canaliculata treated with bio-attractants in indoor experiments

[0043] serial number 4-day mortality rate (%) 4-day adjusted mortality rate (%) Recipe 1 76±5.48 75±5.71a Recipe 2 8±8.37 14.58±8.72d Recipe 3 48±13.04 45.83±13.58c Recipe 4 62±8.37 60.42±8.72b Recipe 5 42±13.04 39.58±13.58c Recipe 6 22±8.37 18.75±8.72d Positive Control 88±8.37 87.5±8.72a Blank control 5±5.48 /

[0044] like Figure 1 and 2 As shown, experiments show that the bio-attractant for golden apple snails of the present invention has the dual effects of physiological killing and plant poisoning. Compared with a single plant extract, the attracting and poisoning effect is significantly improved. Compared with chemical pesticides on the market, it also has good attracting and killing effects, and there is no problem of pesticide residues and non-point source pollution.

[0045] Example 2- Field trapping experiment of Pomacea canaliculata bio-traps with different ratios on Pomacea canaliculata

[0046] Test materials: Same as Example 1

[0047] Experimental method: The experiment was conducted in a rice seedling field in June 2024, with 8 treatments in total, 3 replicates (3 plots) for each treatment, a total of 24 plots, and 2 m 2(2m×1m), the plots were randomly distributed, ridges were built in each plot, and protective nets were built on the ridges to prevent the escape of golden apple snails. Ten healthy golden apple snails of the same size (about 2.5cm in length) were evenly placed in each plot. 9g of golden apple snail biological attractant was evenly applied to each plot. 1.5g of tetraacetaldehyde granules were applied to each plot according to the pesticide instructions for the positive control, and no pesticide was applied to the blank control group. The mortality rate and corrected mortality rate of golden apple snails were investigated 4 days after the application of the pesticide. The control effect was judged based on the difference between the mortality rate of the control group and the mortality rate of the treatment group. The calculation results are shown in Table 3.

[0048] Data processing:

[0049] Mortality rate = number of deaths / total number × 100%

[0050] Adjusted mortality rate = (mortality rate of experimental group - mortality rate of control group) / (1 - mortality rate of control group) × 100%

[0051] Table 3 Mortality of Pomacea canaliculata treated with bio-attractants in field experiment

[0052]

[0053]

[0054] like Figure 3 and 4 As shown, the experiment shows that when the biological control agent of the present invention is used in the field, the probability of attracting and the killing effect are lower than those indoors due to the complex chemical substances in the paddy field. The killing effect of formula 1 is significantly improved compared with the treatment without attractant in formula 6, and the killing effect of the Pomacea canaliculata using the biological attractant of formula 1 is not much different from that of the metaldehyde granules.

[0055] The above is only a preferred embodiment of the present invention. It should be understood that the present invention is not limited to the form disclosed herein, and should not be regarded as excluding other embodiments, but can be used in various other combinations, modifications and environments, and can be modified within the scope of the concept described herein through the above description or the technology of the related field. The changes and modifications made by those skilled in the art shall be within the scope of protection of the claims attached to the present invention as long as they do not deviate from the core technology and scope of the present invention.

Claims

1. A bio-attractant for Pomacea canaliculata, characterized in that: The invention comprises the following ingredients: 100 parts of croton fruit powder, 100 parts of attapulgite, 3 parts of adhesive, 700 parts of attractant and 100 parts of auxiliary materials, wherein the adhesive is sodium polyacrylate solution, and the sodium polyacrylate and water are mixed in a ratio of 1:333 to prepare 0.003 g / mL sodium polyacrylate solution, and the attractant comprises yeast powder, cooked soybean powder, raw soybean powder, corn powder and soybean oil.

2. A bio-attractant for Pomacea canaliculata according to claim 1, characterized in that: The attractant consists of cooked soybean powder, raw soybean powder, yeast powder, corn powder and soybean oil in a compound mass ratio of 25:13:2:29:

1.

3. The bio-attractant for Pomacea canaliculata according to claim 1, characterized in that: The auxiliary material comprises wood cellulose, butyl paraben and rice straw powder in a mass ratio of 1:1:

2.

4. A method for preparing a bio-attractant for Pomacea canaliculata, for preparing the bio-attractant for Pomacea canaliculata as claimed in any one of claims 1 to 3, characterized in that: S1. Grind the croton fruit into 40-60 mesh to obtain croton fruit powder; S2. Place the attractant, croton fruit powder and sodium polyacrylate aqueous solution in an extrusion granulator for granulation. The granulated particles have a length of 3.0 to 4.0 mm, a width of 2 to 3 mm, a moisture content of 40%, and are dried at below 50°C or in the sun.

5. Use of the bio-attractant for controlling Pomacea canaliculata in rice fields and wetlands according to any one of claims 1 to 3, characterized in that: The Pomacea canaliculata trapping agent is evenly spread in the irrigation canal of the rice seedling field, with the dosage of 2-3 kg per mu of the rice seedling field and 1 kg per m2 of the irrigation area. 2 Apply 60-70g and spread it every 10-20 days.

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