VND gene promoter with vascular specificity and application of VND gene promoter in driving expression of xylan hydrolase in plants
By using the SbNAC31 gene promoter specifically expressed in sorghum vascular tissue to drive the expression of xylan hydrolase, the problem of defects in the growth and development of xylan hydrolase mutants in plants was solved, and the recovery of growth and development and the retention of lignocellulose quality was achieved.
CN119823990AActive Publication Date: 2025-04-15INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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Patent Information
- Application Number
- CN202411981778.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2025-04-15
- Estimated Expiration
- 2044-12-31
AI Technical Summary
Technical Problem
The prior art is difficult to achieve specific expression of specific genes in vascular tissues in plants, resulting in defects in growth and development of xylan hydrolase mutants.
Method used
The SbNAC31 gene promoter specifically expressed in sorghum vascular tissue drives the expression of xylan hydrolase, and the gene is combined into the plant expression vector through recombinant vector to achieve specific expression in vascular tissue.
Benefits of technology
The growth and development phenotype of xylan hydrolase deficient plants was successfully restored, while retaining the excellent lignocellulose quality of the mutant, improving the digestibility of the feed.
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Abstract
The invention discloses a vascular specific VND gene promoter and application of the vascular specific VND gene promoter in driving expression of xylan hydrolase in plants. The invention provides a DNA fragment. The DNA fragment is any one of the following: 1) the nucleotide sequence of the DNA fragment is sequence 1; and 2) a nucleic acid sequence which has more than 90% of homology with the nucleic acid sequence shown in 1) and has the same function. Experiments prove that a SbNAC31 gene promoter specifically expressed by a vascular tissue is connected with CDS of a xylan hydrolase gene SbXyl, and a vector with the fusion gene is used for converting a sorghum xylan hydrolase function deletion mutant, so that the growth and development defects of the mutant are made up; meanwhile, the excellent lignocellulose quality (high feed quality) of the mutant is reserved.
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