Cucumber CsCCD1 gene and application thereof in regulating and controlling cucumber pulp color
By cloning and editing the cucumber CsCCD1 gene, the problems of regulating the color of cucumber flesh and improving the content of carotenoids were solved, and the yellowing of the pulp color and significant improvement of carotenoid accumulation were achieved, providing new genetic resources for the improvement of cucumber variety.
CN119913117AActive Publication Date: 2025-05-02INSTITUTE OF VEGETABLES & FLOWERS CHINESE ACADEMY OF AGRICULTURAL SCIENCES +1
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Patent Information
- Application Number
- CN202510237198.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-01
- Publication Date
- 2025-05-02
- Estimated Expiration
- 2045-03-01
AI Technical Summary
Technical Problem
The prior art is difficult to effectively regulate the color of cucumber flesh, especially to increase the content of carotenoids in cucumbers to form yellow or orange flesh.
Method used
By cloning the CsCCD1 gene in cucumber and editing it using CRISPR-Cas9 technology, it leads to mutation of the CsCCD1 gene, thereby inhibiting its expression and activity, and thus regulating the color of the flesh and the accumulation of carotenoids.
Benefits of technology
The color of the cucumber pulp has been significantly yellowed and the carotenoid content of the fruit has been significantly improved, providing new genetic resources and theoretical foundations, laying the foundation for the targeted improvement of cucumber varieties and the cultivation of high-quality new varieties.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention discloses a cucumber CsCCD1 gene and application of the cucumber CsCCD1 gene in regulating and controlling the color of cucumber pulp. According to the invention, a CCD family gene CsCCD1 is cloned from cucumber, and the cucumber CsCCD1 gene has the function of regulating and controlling the pulp color for the first time. A coding region of the CsCCD1 gene is edited by utilizing a CRISPR-Cas9 technology, and after the CsCCD1 is mutated, the color of the cucumber pulp is obviously yellowed, and the carotenoid content of the fruit is obviously improved. The invention not only lays an important foundation for revealing genetic and biological mechanisms of cucumber pulp color, but also provides a new gene resource for directional improvement of cucumber varieties and cultivation of high-quality new varieties, lays a theoretical foundation for later molecular breeding work, and has important application value.
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