Composition and application for detecting the content of PRB1 in saliva of infants
Through the method of combining TFIIB or biotinylated TFIIB with SPR chip, surface plasmon resonance sensors are used to solve the problem of difficult to distinguish and detect PRB1, PRB3, PRH1 and PRB4 content in children's saliva in the prior art, achieving high sensitivity and accuracy PRB1 detection, supporting the prediction of caries symptoms.
CN120064671BActive Publication Date: 2025-07-29JILIN UNIVERSITY
2 Cites 0 Cited by
Patent Information
- Application Number
- CN202510550349.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-29
- Publication Date
- 2025-07-29
- Estimated Expiration
- 2045-04-29
AI Technical Summary
Technical Problem
The prior art is difficult to effectively distinguish and detect the content of PRB1, PRB3, PRH1 and PRB4 in young children's saliva, resulting in inaccurate detection results and affecting the assessment of caries risk.
Method used
TFIIB or biotinylated TFIIB is used to combine with carboxymethylglucanized SPR chip, and the surface of the chip is activated by EDC and NHS, combined with horseradish peroxidase labeling avidin, to achieve specific detection and distinction of PRB1.
Benefits of technology
It realizes high sensitivity, accuracy and reproducibility detection of PRB1 content in young children's saliva, which can effectively distinguish PRB1 from other saliva with proline-rich protein, providing solid support for predicting dental caries symptoms.
✦ Generated by Eureka AI based on patent content.
Abstract
The present invention relates to the field of detection of the content of acidic proline-rich protein in children's saliva, and specifically discloses a composition for detecting the content of PRB1 in children's saliva and its application. The composition provided by the present invention contains TFIIB or a biotin-labeled TFIIB fusion protein. Using the surface plasmon resonance sensor detection method, it can not only effectively detect the content of PRB1 in children's saliva, but also effectively distinguish PRB1 from the other three saliva proline-rich proteins. Moreover, the detection sensitivity, accuracy and reproducibility of the content of PRB1 in children's saliva are higher than those of existing kits, providing solid technical support for predicting and diagnosing the symptoms of dental caries in children.
Need to check novelty before this filing date? Find Prior Art