Efficient acclimatization and transplantation method for sweet potato virus-free test-tube plantlets

CN120548937APending Publication Date: 2025-08-29XUZHOU INST OF AGRI SCI IN JIANGSU XUHUAI DISTRICT (JIANGSU XUZHOU SWEETPOTATO CENT)
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Patent Information

Application Number
CN202510782358.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-06-12
Publication Date
2025-08-29

AI Technical Summary

Technical Problem

然而，随着广大种植户对脱毒种苗的充分认可，也发现广大种植户不具备脱毒试管苗移栽技术，无法直接从脱毒种苗培养单位引进高代脱毒种苗进行扩繁利用，而只能从育苗企业购置生产用苗进行生产利用

Benefits of technology

[0012] The invention has the beneficial effects of reducing the transplanting age of virus-free sweet potato test tube seedlings to 20-30 days, saving costs, significantly improving the transplanting survival rate, breaking through the key technical barriers from test tube seedlings to propagated seedlings, facilitating the rapid application of virus-free test tube seedlings in production, and meeting the needs of seed potato seedling breeding enterprises to provide high-generation virus-free seedlings, as well as meeting the needs of large-scale growers to construct seedling nurseries. Compared with existing methods, the invention has good repeatability, high technical maturity, and a clear and simple operation process.

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Abstract

The invention discloses an efficient acclimatization and transplantation method for sweet potato virus-free test-tube plantlets, which comprises the following steps: removing fibrous roots from sweet potato virus-free test-tube plantlets which are subcultured for 20-30 days through virus detection, treating the sweet potato virus-free test-tube plantlets for 24 hours through an acclimatization nutrient solution, directly planting the sweet potato virus-free test-tube plantlets into a transplantation matrix, keeping the temperature of a culture area at 28 + / -2 DEG C, keeping the relative humidity at 75-80%, and culturing for 3 days under weak light, and supplementing carbon dioxide to the culture area until the concentration is 1000-1200ppm, culturing for 20-30 days under normal illumination, transferring the sweet potato virus-free test-tube plantlet with the survival zone transplanting matrix into a hole tray, filling a seedling culture matrix to ensure that each hole of the matrix is full, watering, carrying out shading treatment for 3-5 days, and transferring to a normal illumination condition for culturing, so as to obtain the sweet potato virus-free test-tube plantlet. According to the method, the seedling age of virus-free seedling transplanting is reduced to 20-30 days, the cost is saved, the transplanting survival rate is remarkably increased, the key technical obstacle from test-tube plantlets to propagation seedlings is broken through, and the sweet potato virus-free test-tube plantlets can be quickly applied to production.
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