Electrochemical aptamer sensor for detecting dibutyl phthalate as well as preparation method and application of electrochemical aptamer sensor
By constructing an electrochemical aptamer sensor based on Fe3O4 magnetic nanoparticles and Hemin@HKUST-1 composite material, the problem of insufficient sensitivity and selectivity in the detection of phthalates in the prior art has been solved, and rapid detection of DBP with high sensitivity and high selectivity has been achieved, which is suitable for trace analysis of food and environmental samples.
Patent Information
- Application Number
- CN202511333972.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-18
- Publication Date
- 2025-12-12
AI Technical Summary
Existing methods for detecting phthalic acid esters (PAEs) suffer from low sensitivity, limited linear range, unoptimized material assembly and surface modification, and insufficient repeatability and stability, making it difficult to meet the need for rapid detection of trace amounts of DBP in complex matrices.
A highly sensitive and selective electrochemical aptamer sensor was constructed by using Fe3O4 magnetic nanoparticles, heme-encapsulated MOF material (Hemin@HKUST-1), and reduced graphene oxide (rGO) modified electrodes. By combining Fe3O4 magnetic nanoparticles with Hemin@HKUST-1, the aptamer-modified magnetic nanoparticles are used for target enrichment, complementary strand DNA hybridization forms signal regulation, and is fixed on the surface of rGO-modified glassy carbon electrode to achieve specific recognition and signal amplification of DBP.
It achieves highly sensitive and selective detection of dibutyl phthalate (DBP) with a detection range of 3×10⁻⁶ to 3×10⁻⁃ mg/mL, a linear correlation coefficient R² of 0.99259, and a method detection limit as low as 2.90×10⁻⁶ mg/mL. It exhibits good repeatability and stability and can accurately detect DBP in complex matrices.