Bordetella pertussis engineered strain, preparation method and application thereof

CN121653033BActive Publication Date: 2026-07-24SHANGHAI YUGUAN BIOTECH CO LTD
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Patent Information

Application Number
CN202610179036.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2026-02-09
Publication Date
2026-07-24
Estimated Expiration
2046-02-09

AI Technical Summary

Technical Problem

Bordetella pertussis is unable to efficiently secrete outer membrane vesicles (OMV), which limits the development of pertussis OMV vaccines and the effectiveness of immune responses.

Method used

OMV production was increased by introducing the expression of lpxD protein derived from Pseudomonas jinjuensis into Bordetella pertussis. The specific method involved constructing a nucleic acid construct and performing gene editing in Bordetella pertussis to express the lpxD protein to enhance OMV secretion.

Benefits of technology

It significantly improved the yield and quality of Bordetella pertussis OMV, enhanced the effectiveness of the immune response, and promoted the development of a Bordetella pertussis OMV vaccine.

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Abstract

This invention discloses an engineered strain of Bordetella pertussis, its preparation method, and its application. The engineered Bordetella pertussis strain and the Bordetella pertussis strain (…) Bordetella pertussis Compared to ), expressing Jinzhou Pseudomonas ( Pseudomonas jinjuensis The lpxD protein is derived from [source missing]. The strain of Bordetella pertussis engineered strain provided by this invention has a significant effect on increasing OMV production, which will promote the development of Bordetella pertussis OMV vaccines.
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Citation Information

Patent Citations

  • OmpA mutation enhanced OMV production in Bordetella pertussis

    CN116438193A

  • Modified Bordetella pertussis strains

    CN118853525B

  • Vaccines for the prevention of infections with Bordetella

    US9623102B2

  • Genetically intervened bordetella pertussis strain as well as preparation method and application thereof

    CN121271771A