Deodorizing and bacteriostatic preparation for livestock and poultry farms and preparation method of deodorizing and bacteriostatic preparation

Abstract

The invention discloses a deodorization and bacteriostasis preparation for livestock and poultry farms and a preparation method of the deodorization and bacteriostasis preparation in the technical field of deodorization and bacteriostasis, and the deodorization and bacteriostasis preparation is a mixture of a hypochlorous acid aqueous solution, a polygonatum odoratum extract, platycladus orientalis oil, tocopheryl acetate, polyglycerol-4 oleate, disodium hydrogen phosphate and a surfactant, the polygonatum odoratum extract is a product obtained after polygonatum odoratum is fermented through compound probiotics, and the preparation method comprises the steps that low-temperature freezing and smashing treatment is conducted on polygonatum odoratum, polygonatum odoratum powder is obtained, then the compound probiotics and fermentation auxiliary materials are added for fermentation, and the polygonatum odoratum extract is obtained after the fermentation product is sequentially centrifuged, filtered and concentrated and dried. According to the deodorization and bacteriostasis preparation for the livestock and poultry farm, the hypochlorous acid, the radix polygonati officinalis extract and the platycladus orientalis oil are compounded, so that the deodorization and bacteriostasis preparation has a good bacteriostasis effect and low-temperature adaptability, and the bacteriostasis stability of the deodorization and bacteriostasis preparation with the hypochlorous acid as a core is effectively improved.

Description

Technical Field

[0001] This invention relates to the field of deodorization and antibacterial technology, specifically to a deodorization and antibacterial preparation for livestock and poultry farms and its preparation method. Background Technology

[0002] Currently, there are many types of deodorizing and antibacterial products available on the market for livestock and poultry farms, which can be mainly divided into three categories: chemical preparations, biological preparations, and plant-derived preparations. Chlorine-containing disinfectants belong to a common type of chemical preparation. These disinfectants have the advantages of rapid effect, broad bactericidal spectrum, and wide applicability. However, traditional hypochlorous acid disinfectants are chemically unstable and are greatly affected by light, temperature, and pH value. In order to improve the effectiveness and stability of disinfectants with hypochlorous acid as the core, existing technologies have carried out multi-directional research and development on hypochlorous acid compound disinfectants.

[0003] Chinese Patent Publication No. CN119174426B discloses a hypochlorous acid composite disinfectant and its preparation method. In this composite disinfectant, the synergistic effect of *Aristolochia debilis* extract and hypochlorous acid enhances the disinfection effect, broadens the disinfection spectrum, and improves disinfection efficiency. Furthermore, the use of polyethylene glycol and phosphates improves the stability of the disinfectant, allowing it to maintain good bactericidal properties even after 14 days of constant temperature and humidity storage at 54°C. However, while this hypochlorous acid composite disinfectant considers the impact of high temperatures on the stability of hypochlorous acid, it does not address the effect of low temperatures on the bactericidal performance of hypochlorous acid. Low temperatures not only delay the disinfection efficiency of hypochlorous acid but may even directly lead to a significant decrease in its bactericidal performance. Therefore, this invention proposes a deodorizing and antibacterial agent for livestock and poultry farms and its preparation method. Summary of the Invention

[0004] The purpose of this invention is to provide a deodorizing and antibacterial agent for livestock and poultry farms and its preparation method in order to solve the above-mentioned problems.

[0005] The present invention achieves the above objectives through the following technical solutions: This invention provides a deodorizing and antibacterial preparation for livestock and poultry farms, which is a mixture of hypochlorous acid aqueous solution, Solomon's seal extract, arborvitae oil, tocopheryl acetate, polyglycerol-4 oleate, disodium hydrogen phosphate, and surfactant. Among them, the Solomon's Seal Extract is a product of Solomon's Seal fermented with compound probiotics.

[0006] As a further optimization of the present invention, the deodorizing and antibacterial preparation comprises, by weight, 50-60 parts of hypochlorous acid aqueous solution, 5-8 parts of Solomon's seal extract, 1-3 parts of arborvitae oil, 8-15 parts of polyglycerol-4 oleate, 3-4 parts of surfactant, 1-2 parts of disodium hydrogen phosphate and 1-2 parts of tocopherol acetate.

[0007] As a further optimization of the present invention, the compound probiotics include Lactobacillus plantarum and Bacillus subtilis in a mass ratio of 1:2.

[0008] As a further optimization of the present invention, the surfactant is a 1:1 mixture of lauryl sulfate monoethanolamine and Tween 80.

[0009] A method for preparing a deodorizing and antibacterial agent for livestock and poultry farms, specifically including the following steps: (1) To prepare Solomon's seal extract, Solomon's seal extract was mixed with Platycladus orientalis oil, and then the mixture was added to polyglycerol-4 oleate and stirred until completely dissolved to obtain premix A; (2) Mix the surfactant and tocopheryl acetate evenly to obtain premix B; (3) Disodium hydrogen phosphate is uniformly mixed into hypochlorous acid aqueous solution to obtain premixed solution C; (4) Add premix A to premix B and then perform ultrasonic stirring to obtain a uniform dispersion. Add premix C to the dispersion and stir thoroughly to obtain a deodorizing and antibacterial agent. As a further optimization of the present invention, in step (4), the conditions for ultrasonic stirring are: 350-450W, 20-40kHz, 100-300rpm, 5-10min.

[0010] As a further optimization of the present invention, the preparation method of Solomon's Seal Extract is as follows: Solomon's Seal is subjected to low-temperature freeze-pulverization to obtain Solomon's Seal powder, then compound probiotics and fermentation excipients are added for fermentation, and the fermentation product is centrifuged, filtered and concentrated and dried in sequence to obtain Solomon's Seal Extract.

[0011] As a further optimization of the present invention, the inoculation amount of compound probiotics is 5% of the mass of Solomon's seal powder, and the amount of fermentation excipients added is 10% of the mass of Solomon's seal powder, which includes glucose and yeast extract in a mass ratio of 1:2. The fermentation conditions are: anaerobic fermentation at 30°C for 24 hours, with continuous stirring at 100 rpm during the fermentation process.

[0012] The beneficial effects of this invention are as follows: The method for preparing deodorizing and antibacterial agents for livestock and poultry farms proposed in this invention uses hypochlorous acid as the core deodorizing and antibacterial component, combined with the synergistic assistance of Solomon's seal extract and arborvitae oil. The resulting deodorizing and antibacterial agent not only has good deodorizing and antibacterial properties, but also excellent low-temperature adaptability. It can maintain good antibacterial efficiency and obtain excellent antibacterial effects even in low-temperature environments. The agent has stable effects and a wide range of applications, effectively improving the practical value of deodorizing and antibacterial agents in the hygiene management of livestock and poultry farms. Detailed Implementation

[0013] The present application will now be described in further detail. It should be noted that the following specific embodiments are only used to further illustrate the present application and should not be construed as limiting the scope of protection of the present application. Those skilled in the art can make some non-essential improvements and adjustments to the present application based on the above application content.

[0014] I. Experimental Materials (1) In this invention, Lactobacillus plantarum and Bacillus subtilis were activated at 37°C for 12 hours using MRS liquid medium and LB liquid medium, respectively, to obtain corresponding bacterial solutions, in which the viable bacterial count was approximately 10. 8 CFU / mL; (2) In this invention, the effective chlorine concentration of the hypochlorous acid aqueous solution is 50~200mg / L. In the following examples, a hypochlorous acid aqueous solution of 100mg / L is used.

[0015] Unless otherwise specified, all methods used in this invention are conventional methods known to those skilled in the art, and all reagents and materials used are commercially available products.

[0016] Preparation and efficacy testing of deodorizing and antibacterial agents 1. Preparation of deodorizing and antibacterial agents Example 1 A deodorizing and antibacterial agent for livestock and poultry farms, the preparation method of which includes the following steps: (1) Low-temperature freeze-pulverization treatment of Polygonatum odoratum (-20℃, 150 mesh) was performed to obtain Polygonatum odoratum powder. The activated Lactobacillus plantarum bacterial solution and Bacillus subtilis bacterial solution were mixed at a mass ratio of 1:2 to obtain compound probiotics. Glucose and yeast extract were mixed at a mass ratio of 1:2 to obtain fermentation excipients. 10% of its own mass of fermentation excipients was added to Polygonatum odoratum powder, and then 5% of its own mass of compound probiotics was inoculated. The entire fermentation system was subjected to anaerobic fermentation at 30℃, 100 rpm and 24 h. The fermentation product was centrifuged, the supernatant was filtered, and the filtrate was concentrated and dried (-0.08 MPa, 65℃ for 40 min) to obtain Polygonatum odoratum extract. Lauryl sulfate monoethanolamine and Tween 80 were mixed at a mass ratio of 1:1 to obtain a surfactant; Take 50 parts by weight of hypochlorous acid aqueous solution, 8 parts of Solomon's seal extract, 3 parts of arborvitae oil, 15 parts of polyglycerol-4 oleate, 4 parts of surfactant, 2 parts of disodium hydrogen phosphate and 1 part of tocopheryl acetate for later use. (2) Mix the Solomon's seal extract with arborvitae oil, then add the mixture to polyglycerol-4 oleate and stir until completely dissolved to obtain premix A; (3) Mix the surfactant and tocopheryl acetate evenly to obtain premix B; (4) Disodium hydrogen phosphate is uniformly mixed into the hypochlorous acid aqueous solution to obtain premixed solution C; (5) Add premix A to premix B, and then perform ultrasonic stirring at 350W, 40kHz, 100rpm and 10min to obtain a uniform dispersion. Add premix C to the dispersion and stir thoroughly to obtain a deodorizing and antibacterial agent.

[0017] Example 2 A deodorizing and antibacterial agent for livestock and poultry farms, the preparation method of which includes the following steps: (1) Prepare Polygonatum odoratum extract and surfactant (method is the same as in Example 1). Take 60 parts by weight of hypochlorous acid aqueous solution, 5 parts of Polygonatum odoratum extract, 1 part of Platycladus orientalis oil, 8 parts of polyglycerol-4 oleate, 3 parts of surfactant, 1 part of disodium hydrogen phosphate and 1 part of tocopherol acetate for later use. (2) Mix the Solomon's seal extract with arborvitae oil, then add the mixture to polyglycerol-4 oleate and stir until completely dissolved to obtain premix A; (3) Mix the surfactant and tocopheryl acetate evenly to obtain premix B; (4) Disodium hydrogen phosphate is uniformly mixed into the hypochlorous acid aqueous solution to obtain premixed solution C; (5) Add premix A to premix B, and then perform ultrasonic stirring at 450W, 20kHz, 300rpm for 5min to obtain a uniform dispersion. Add premix C to the dispersion and stir thoroughly to obtain a deodorizing and antibacterial agent.

[0018] Example 3 A deodorizing and antibacterial agent for livestock and poultry farms, the preparation method of which includes the following steps: (1) Prepare Polygonatum odoratum extract and surfactant (method is the same as in Example 1). Take 55 parts of hypochlorous acid aqueous solution, 7 parts of Polygonatum odoratum extract, 2 parts of Platycladus orientalis oil, 11 parts of polyglycerol-4 oleate, 3.5 parts of surfactant, 1.5 parts of disodium hydrogen phosphate and 1.5 parts of tocopherol acetate by weight for later use. (2) Mix the Solomon's seal extract with arborvitae oil, then add the mixture to polyglycerol-4 oleate and stir until completely dissolved to obtain premix A; (3) Mix the surfactant and tocopheryl acetate evenly to obtain premix B; (4) Disodium hydrogen phosphate is uniformly mixed into the hypochlorous acid aqueous solution to obtain premixed solution C; (5) Add premix A to premix B, and then perform ultrasonic stirring at 400W, 30kHz, 150rpm for 8min to obtain a uniform dispersion. Add premix C to the dispersion and stir thoroughly to obtain a deodorizing and antibacterial agent.

[0019] Comparative Example 1 A deodorizing and antibacterial agent for livestock and poultry farms, the preparation method of which includes the following steps: (1) Mix monoethanolamine lauryl sulfate and Tween 80 at a mass ratio of 1:1 to obtain a surfactant. Take 64 parts of hypochlorous acid aqueous solution, 11 parts of polyglycerol-4 oleate, 3.5 parts of surfactant, 1.5 parts of disodium hydrogen phosphate and 1.5 parts of tocopherol acetate by mass for later use. (2) Use polyglycerol-4 oleate as premix A, mix the surfactant and tocopheryl acetate evenly to obtain premix B, and mix disodium hydrogen phosphate evenly into hypochlorous acid aqueous solution to obtain premix C. (3) Add premix A to premix B, and then perform ultrasonic stirring at 400W, 30kHz, 150rpm for 8min to obtain a uniform dispersion. Add premix C to the dispersion and stir thoroughly to obtain a deodorizing and antibacterial agent.

[0020] Comparative Example 2 Based on Example 3, the deodorizing and antibacterial preparation was prepared by simply replacing the Solomon's seal extract used in step (2) with an equal amount of arborvitae oil.

[0021] Comparative Example 3 Based on Example 3, the arborvitae oil used in step (2) was replaced with an equal amount of Solomon's seal extract to prepare a deodorizing and antibacterial preparation.

[0022] Comparative Example 4 Based on Example 3, the arborvitae oil used in step (2) was replaced with an equal amount of arborvitae leaf oil to prepare a deodorizing and antibacterial preparation.

[0023] Comparative Example 5 Based on Example 3, the arborvitae oil used in step (2) was replaced with an equal amount of tea tree oil to prepare a deodorizing and antibacterial preparation.

[0024] 2. Efficacy testing of deodorizing and antibacterial agents Samples were taken from the deodorizing and antibacterial preparations obtained in Examples 1-3 and tested. The specific test items and methods are as follows. (1) Deodorization experiment Pig manure was added to a sealed container and the sample was sprayed. The dosage was 5 mL for every 50 g of manure. At the same time, a control group was set up by spraying an equal amount of pure water. After 2 hours, the concentrations of ammonia and hydrogen sulfide in each group's container were measured using a gas detector. The deodorization rate was calculated based on the concentration changes. The formula is: Deodorization rate = [(Control group concentration - Experimental group concentration) / Control group concentration] × 100.

[0025] (2) Experiment on the antibacterial effect of disinfectant ① Experimental strains: Escherichia coli (ATCC8099) and Candida albicans (CMCC(F)98001), purchased from the China Institute of Veterinary Drug Control, were used as experimental strains. The experimental strains were inoculated on BHI agar plates by streak plating and incubated at 37°C for 24 hours. Several single colonies with similar morphology and a diameter of about 1-3 mm were picked and placed in 5 mL of physiological saline. The colonies were ground and shaken well. The concentration was corrected to 0.5 McFarland turbidity units on a turbidimeter for later use. ② To determine the initial colony count of the standard bacterial suspension, mix 0.5 mL of the standard bacterial suspension with 4.5 mL of the sample to obtain a reaction solution. Place the reaction solution in an incubator at 5℃ and 25℃ respectively. At reaction times of 10 min, 30 min, and 60 min, take 0.5 mL of the reaction solution to determine the colony count. The assay method was as follows: Under a water bath at 20℃, 4.5 mL of neutralizing agent solution was added to 0.5 mL of the reaction solution in a test tube, and neutralization was allowed to proceed for 15 min to obtain a mixed solution. The mixed solution was then diluted using the tenfold dilution method and the plate counting method, and spread onto plates. After incubation at 37℃ for 36 h, the colony count was observed and counted, and the sterilization rate was calculated. Each experiment was performed three times, and the average value was taken.

[0026] 3. Test Results The test results are shown in Table 1-4. From the table, we can see that: (1) According to the data of Examples 1-3 and the data of Example 3 compared with Comparative Example 1, it can be seen that in this invention, by adding Polygonatum odoratum extract and Platycladus orientalis oil during the preparation process, the antibacterial effect and low temperature adaptability of the deodorizing and antibacterial preparation are significantly improved. Specifically, on the one hand, under the condition of 25°C, after 60 minutes of action, the deodorizing and antibacterial agent can achieve an antibacterial rate of over 99% against Escherichia coli and over 98.7% against Candida albicans, which is significantly better than Comparative Example 1; on the other hand, under the condition of 5°C, although the antibacterial effect of the deodorizing and antibacterial agent in Example 3 showed a certain lag, its antibacterial efficiency was still significantly higher than that of Comparative Example 1, and after 60 minutes of action, the deodorizing and antibacterial agent could achieve an antibacterial rate of over 99%.

[0027] (2) Compared with Example 3, the antibacterial properties and low-temperature adaptability of the deodorizing and antibacterial agents obtained in Comparative Examples 2-5 decreased to varying degrees. Among them, the decrease was more significant in Comparative Examples 2 and 3. Although the antibacterial rate of the deodorizing and antibacterial agents after 60 minutes of action was similar to that in Example 3, the antibacterial efficiency was significantly worse than that in Example 3 in Comparative Examples 4 and 5. The above results indicate that the advantages of the deodorizing and antibacterial agents proposed in this invention depend on the synergy of Polygonatum odoratum extract and Platycladus orientalis oil, and the combination of Polygonatum odoratum extract or Platycladus orientalis oil has a significant advantage in improving the antibacterial efficiency of the deodorizing and antibacterial agents under low-temperature conditions.

[0028] Table 1. Deodorizing effect of deodorizing and antibacterial agents ; Table 2. Antibacterial effect of deodorizing and antibacterial agents at 25℃ ; Table 3. Antibacterial effect of deodorizing and antibacterial agents at 5℃ ; The embodiments described above are merely examples of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention.

Claims

1. A deodorizing and antibacterial agent for livestock and poultry farms, characterized in that: The deodorizing and antibacterial agent is a mixture of hypochlorous acid aqueous solution, Solomon's seal extract, arborvitae oil, tocopheryl acetate, polyglycerol-4 oleate, disodium hydrogen phosphate, and surfactant. The Solomon's Seal Extract is a product of Solomon's Seal fermented with compound probiotics.

2. The deodorizing and antibacterial agent for livestock and poultry farms according to claim 1, characterized in that: The deodorizing and antibacterial preparation comprises, by weight, 50-60 parts of hypochlorous acid aqueous solution, 5-8 parts of Solomon's seal extract, 1-3 parts of arborvitae oil, 8-15 parts of polyglycerol-4 oleate, 3-4 parts of surfactant, 1-2 parts of disodium hydrogen phosphate and 1-2 parts of tocopheryl acetate.

3. The deodorizing and antibacterial agent for livestock and poultry farms according to claim 1, characterized in that: The compound probiotics include Lactobacillus plantarum and Bacillus subtilis in a mass ratio of 1:

2.

4. The deodorizing and antibacterial agent for livestock and poultry farms according to claim 1, characterized in that: The surfactant is a 1:1 mixture of lauryl sulfate monoethanolamine and Tween 80.

5. A method for preparing a deodorizing and antibacterial agent for livestock and poultry farms as described in claims 1-4, characterized in that: Specifically, the following steps are included: (1) To prepare Solomon's seal extract, Solomon's seal extract was mixed with Platycladus orientalis oil, and then the mixture was added to polyglycerol-4 oleate and stirred until completely dissolved to obtain premix A; (2) Mix the surfactant and tocopheryl acetate evenly to obtain premix B; (3) Disodium hydrogen phosphate is uniformly mixed into hypochlorous acid aqueous solution to obtain premixed solution C; (4) Add premix A to premix B and then perform ultrasonic stirring to obtain a uniform dispersion. Add premix C to the dispersion and stir thoroughly to obtain a deodorizing and antibacterial agent.

6. The preparation method according to claim 5, characterized in that: In step (4), the conditions for ultrasonic stirring are: 350-450W, 20-40kHz, 100-300rpm, 5-10min.

7. The preparation method according to claim 5, characterized in that: The preparation method of the Solomon's Seal Extract is as follows: Solomon's Seal is subjected to low-temperature freeze-pulverization to obtain Solomon's Seal powder, then compound probiotics and fermentation excipients are added for fermentation, and the fermentation product is centrifuged, filtered and concentrated and dried in sequence to obtain Solomon's Seal Extract.

8. The preparation method according to claim 7, characterized in that: The inoculation amount of the compound probiotic is 5% of the mass of the Solomon's Seal powder, and the amount of the fermentation auxiliary material added is 10% of the mass of the Solomon's Seal powder, which includes glucose and yeast extract in a mass ratio of 1:

2. The fermentation conditions are: anaerobic fermentation at 30°C for 24 hours, with continuous stirring at 100 rpm during the fermentation process.