An integrated microfield of view cochlear basement membrane dissection instrument platform

Abstract

This invention relates to the field of cochlear basilar membrane anatomy technology with integrated microscopic vision, and discloses an integrated microscopic cochlear basilar membrane anatomy instrument platform, including a fixing mechanism fixedly connected to the inner wall of the groove to reduce the height difference between the liquid and the outlet during liquid extraction. A storage mechanism rotatably connected to the inner wall of the fixing mechanism stores the liquid. A deceleration mechanism fixedly connected to the outer wall of the fixing mechanism slows down the movement speed of the device. This prevents hydrostatic pressure loss as the liquid level drops, which would result in insufficient initial force for the liquid to enter the outlet tube, slowing down the liquid delivery efficiency, preventing timely replenishment of the physiological solution, causing osmotic pressure imbalance in the sample, and ultimately deforming the sample, rendering it unusable.

Description

Technical Field

[0001] This invention relates to the field of cochlear basilar membrane dissection instruments and equipment with integrated microscopic vision, specifically a cochlear basilar membrane dissection instrument platform with integrated microscopic vision. Background Technology

[0002] When dissecting the cochlear basilar membrane, the physiological solution is first prepared, then slowly delivered into a culture dish using a peristaltic pump, and finally the cochlear basilar membrane is dissected under a microscope.

[0003] When a peristaltic pump delivers physiological solution into a reservoir, the static pressure of the liquid decreases as the liquid level decreases. Static pressure is the pressure exerted by the liquid under its own weight when it is at rest or flowing slowly, and it is also the basic pressure that drives the liquid to flow steadily. The loss of static pressure results in insufficient initial thrust for the liquid to enter the tubing. Even if the peristaltic pump speed remains constant, more power is required to overcome the tubing resistance, resulting in a decrease in actual delivery efficiency. Ultimately, this leads to osmotic pressure imbalance and causes the sample to deform. Summary of the Invention

[0004] To solve the above technical problems, the present invention provides an integrated microscopic field of view cochlear basilar membrane dissection instrument platform, including an external mechanism, the external mechanism including a box, a movable microscope rotatably connected to the outer wall of the box, a screen rotatably connected to the outer wall of the box, a slot opened on the outer wall of the box, a culture tank opened on the outer wall of the box, a peristaltic pump fixedly connected to the outer wall of the box, and a liquid outlet pipe fixedly connected to the outer wall of the peristaltic pump.

[0005] The fixing mechanism is fixedly connected to the inner wall of the tank opening and is used to reduce the height difference between the liquid and the outlet when the liquid is being drawn.

[0006] A storage mechanism, which is rotatably connected to the inner wall of a fixed mechanism, is used to store liquids;

[0007] The speed reduction mechanism is fixedly connected to the outer wall of the fixed mechanism and is used to slow down the movement speed of the device.

[0008] Preferably, the fixing mechanism includes:

[0009] The fixing component is fixedly connected to the inner wall of the slot.

[0010] The motion component is fixedly connected to the outer wall of the fixed component.

[0011] Preferably, the storage mechanism includes:

[0012] The storage component is rotatably connected to the inner wall of the moving component.

[0013] The rotating component is fixedly connected to the outer wall of the storage component.

[0014] Preferably, the speed reduction mechanism includes:

[0015] The deceleration assembly is fixedly connected to the outer wall of the motion assembly;

[0016] The connecting component is fixedly connected to the inner wall of the slot.

[0017] Preferably, the fixing component includes a fixing plate fixedly connected to the inner wall of the slot, a stop block fixedly connected to the outer wall of the fixing plate, and a rotating strip rotatably connected to the outer wall of the fixing plate.

[0018] Preferably, the motion component includes a rotating plate fixedly connected to the outer wall of the rotating bar, and an outer tank is fixedly connected to the outer wall of the rotating plate.

[0019] Preferably, the storage component includes a rotating disk rotatably connected to the inner wall of the outer tank, an inner tank rotatably connected to the outer wall of the rotating disk, and a slot two being provided on the outer wall of the outer tank.

[0020] Preferably, the rotating assembly includes a fixed column fixedly connected to the outer wall of the inner tank, and a counterweight is rotatably connected to the outer wall of the fixed column.

[0021] Preferably, the deceleration assembly includes a moving plate fixedly connected to the outer wall of the rotating plate, and a locking block is fixedly connected to the outer wall of the moving plate;

[0022] Preferably, the connecting assembly includes a connecting column fixedly connected to the inner wall of the slot, a compression strip slidably connected to the inner wall of the connecting column, a fixed frame slidably connected to the outer wall of the compression strip, a spring fixedly connected to the end of the compression strip away from the moving plate, and a flip plate rotatably connected to the inner wall of the compression strip.

[0023] The present invention has the following beneficial effects:

[0024] (1) When the liquid level inside the inner tank begins to be consumed, the weight of the inner tank itself will begin to decrease. At this time, the elasticity generated by the torsion spring is greater than the weight of the device above the rotating bar. At this time, the rotating bar will begin to tilt away from the abutment block. When the rotating bar begins to tilt, the rotating plate will begin to tilt. When the rotating plate tilts, the inner tank, the outer tank, and the fixed column will also begin to tilt. When the inner tank tilts, the liquid level will not decrease due to the peristaltic pump. Instead, the outlet pipe will always be submerged in liquid. This prevents the static pressure loss that will occur when the liquid level drops as the liquid level is continuously consumed. The static pressure loss will cause the initial force of the liquid entering the outlet pipe to be insufficient, which will slow down the liquid delivery efficiency and cause the physiological solution to be unable to be renewed in time, resulting in sample osmotic pressure imbalance and ultimately sample deformation that makes it unusable.

[0025] (2) When the rotating plate tilts, the moving plate will also tilt. When the moving plate tilts, it will generate a thrust on the compression strip, causing the compression strip to slide inside the connecting column. Because the connecting column is filled with liquid, when the compression strip slides away from the moving plate, the compression strip will start to compress the spring. At this time, the liquid inside the connecting column will start to hit the flip plate. Because the compression strip is equipped with a second locking block, the flip plate, which was originally in a horizontal state, will return to a vertical state, and the flow rate through the compression strip will also decrease. When affected by the spring and the flip plate, the sliding speed of the compression strip will begin to slow down. When the sliding speed of the compression strip slows down, the tilting speed of the moving plate will also begin to slow down, and the tilting speed of the rotating plate and the rotating strip connected to it will also begin to slow down. In this way, it is prevented that the liquid surface will fluctuate greatly when tilting. During the fluctuation, the liquid impacts back and forth in the bottle, forming local turbulence. The shearing force generated by the turbulence will cause the static cilia to bend or the basement membrane to be torn.

[0026] (3) When the fixed column begins to tilt, the fixed column and the counterweight are rotatably connected, so that no matter what position the fixed column tilts to, the counterweight will remain vertically downward. Because the counterweight itself is heavy, and the liquid inside the inner tank is constantly consumed, the inner tank will be affected by the weight of the counterweight itself, and will start to rotate slowly through the rotating disk. This will cause the inner tank to start to rotate on its own center while tilting. In this way, when the inner tank rotates, it guides the liquid inside the inner tank to form a laminar flow, which counteracts the shaking caused by the inertia of the liquid inside the inner tank when it is tilted, and prevents the subsequent fluctuations caused by the inertia of the liquid from causing the hair cells and cilia to bend or break.

[0027] (4) When the rotating plate is tilted, the inner tank also begins to tilt. This causes the residue inside the physiological solution to sink to the end with a smaller tilt angle because the residue is heavier. When the outlet tube is then inserted into the inner tank, the outlet tube will be at the end with a larger tilt angle because the material of the outlet tube is lighter. This prevents the tube and the residue from being in the same place, thus preventing the residue from being directly sucked into the outlet tube when the liquid is sucked in, which would cause blockage inside the outlet tube. Attached Figure Description

[0028] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0029] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0030] Figure 2 This is a cross-sectional view of the overall structure of the present invention;

[0031] Figure 3 For the present invention Figure 2 Enlarged view of point B in the middle;

[0032] Figure 4 This is a schematic diagram of the overall structure of the fixing plate of the present invention;

[0033] Figure 5 For the present invention Figure 4 Enlarged view of point A in the middle;

[0034] Figure 6 This is a schematic diagram showing the location of the deceleration component of the present invention;

[0035] Figure 7 This is a schematic diagram of the back of the outer can of the present invention;

[0036] Figure 8 For the present invention Figure 7 Enlarged diagram of point C in the middle.

[0037] The attached diagram lists the components represented by each number as follows:

[0038] In the diagram: 1. External mechanism; 12. Box body; 13. Slot; 14. Mobile microscope; 16. Screen; 17. Culture tank; 18. Discharge pipe; 19. Peristaltic pump; 2. Fixing mechanism; 21. Fixing component; 211. Fixing plate; 212. Abutment block; 213. Rotating bar; 22. Motion component; 221. Rotating plate; 222. Outer tank; 3. Storage mechanism; 31. Storage component; 311. Rotating disk; 312. Inner tank; 313. Slot II; 32. Rotating component; 321. Counterweight; 322. Fixing column; 4. Reduction mechanism; 41. Reduction component; 411. Motion plate; 412. Clamping block; 413. Compression bar; 42. Connecting component; 421. Connecting column; 422. Flip plate; 423. Spring; 424. Fixing frame. Detailed Implementation

[0039] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0040] Example 1, please refer to Figures 1-6The present invention is an integrated microscopic field of view cochlear basilar membrane dissection instrument platform, including an external mechanism 1, which also includes a box 12. A movable microscope 14 is rotatably connected to the outer wall of the box 12, a screen 16 is rotatably connected to the outer wall of the box 12, a slot 13 is opened on the outer wall of the box 12, a culture tank 17 is opened on the outer wall of the box 12, a peristaltic pump 19 is fixedly connected to the outer wall of the box 12, and a liquid outlet tube 18 is fixedly connected to the outer wall of the peristaltic pump 19.

[0041] Fixing mechanism 2 is fixedly connected to the inner wall of the groove 13 and is used to reduce the height difference between the liquid and the outlet when the liquid is being drawn.

[0042] Storage mechanism 3 is rotatably connected to the inner wall of fixed mechanism 2 and is used to store liquid;

[0043] The deceleration mechanism 4 is fixedly connected to the outer wall of the fixed mechanism 2 and is used to slow down the movement speed of the device.

[0044] Fixed mechanism 2 includes:

[0045] Fixing component 21 is fixedly connected to the inner wall of the slot 13;

[0046] Motion component 22 is fixedly connected to the outer wall of fixed component 21.

[0047] Example 2, please refer to Figures 2-8 This invention is an integrated microscopic cochlear basilar membrane dissection instrument platform. Based on Example 1, the storage mechanism 3 includes:

[0048] Storage component 31 is rotatably connected to the inner wall of motion component 22;

[0049] Rotating component 32 is fixedly connected to the outer wall of storage component 31.

[0050] The speed reduction mechanism 4 includes:

[0051] The deceleration assembly 41 is fixedly connected to the outer wall of the motion assembly 22.

[0052] The connecting component 42 is fixedly connected to the inner wall of the slot 13.

[0053] The fixing component 21 includes a fixing plate 211 fixedly connected to the inner wall of the slot 13, a stop block 212 fixedly connected to the outer wall of the fixing plate 211, and a rotating strip 213 rotatably connected to the outer wall of the fixing plate 211.

[0054] A torsion spring is provided at the intersection of the fixed plate 211 and the rotating bar 213. Affected by the abutment block 212, the rotating bar 213 can only rotate to one side, and when the rotating bar 213 is close to the abutment block 212, the rotating bar 213 will be in a slightly tilted state.

[0055] The motion component 22 includes a rotating plate 221 fixedly connected to the outer wall of the rotating bar 213, and an outer can 222 fixedly connected to the outer wall of the rotating plate 221;

[0056] The rotating bar 213 is connected at a position away from the center of the rotating plate 221.

[0057] Storage component 31 includes a rotating disk 311 rotatably connected to the inner wall of the outer tank 222, an inner tank 312 rotatably connected to the outer wall of the rotating disk 311, and a slot 313 is provided on the outer wall of the outer tank 222.

[0058] The process involves adding a physiological solution into the inner tank 312, and then using a peristaltic pump 19 to extract the liquid from the inner tank 312.

[0059] The rotating assembly 32 includes a fixed column 322 fixedly connected to the outer wall of the inner tank 312, and a counterweight 321 rotatably connected to the outer wall of the fixed column 322;

[0060] When the liquid inside the inner tank 312 begins to be consumed, the potential energy accumulated by the spring at the intersection of the rotating bar 213 and the fixed plate 211 will cause the torsion spring to release its potential energy, causing the moving component 22, the storage component 31, and the rotating component 32 to begin to tilt. When tilting, the counterweight 321 will always remain vertically downward. The vertically downward counterweight 321 will exert a downward gravity on the inner tank 312, causing the inner tank 312 to begin to rotate little by little.

[0061] The deceleration assembly 41 includes a moving plate 411 fixedly connected to the outer wall of the rotating plate 221, and a locking block 412 fixedly connected to the outer wall of the moving plate 411;

[0062] When the rotating plate 221 begins to tilt, the moving plate 411 and the locking block 412 will also begin to tilt.

[0063] The connecting component 42 includes a connecting post 421 fixedly connected to the inner wall of the slot 13, a compression strip 425 slidably connected to the inner wall of the connecting post 421, a fixed frame 424 slidably connected to the outer wall of the compression strip 425, a spring 423 fixedly connected to the end of the compression strip 425 away from the moving plate 411, and a flip plate 422 rotatably connected to the inner wall of the compression strip 425.

[0064] When the moving plate 411 begins to tilt, the compression strip 425, which is attached to the outer wall of the moving plate 411, begins to slide inside the connecting post 421, and at the same time, it compresses the spring 423, causing the spring 423 to accumulate elastic potential energy. Meanwhile, the liquid inside the connecting post 421 causes the flipping plate 422 to change directly from a horizontal state to a vertical state as the compression strip 425 moves. At this time, the sliding speed of the compression strip 425 inside the connecting post 421 will begin to slow down. When the compression strip 425 begins to decelerate, the tilting speed of the moving plate 411 will also begin to slow down.

[0065] A specific application of this embodiment is as follows: When starting the dissection, the operator first presses the tilted rotating bar 213 against the abutment block 212, and then moves the counterweight block 321 from the right end to the left end. At this time, 411 will also return from the tilted state to the vertical state. When 411 no longer squeezes 425, 425 will be pressed against the bottom of 411 again through 423. Then, the required physiological solution is added to the inner tank 312. At this time, the overall weight of the inner tank 312 with added liquid is equal to the deflection force generated by the torsion spring at the intersection of the fixed plate 211 and the rotating bar 213, so that the rotating bar 213 will no longer tilt. Then, the liquid outlet pipe 18 is fixed inside the inner tank 312, and then another liquid outlet pipe 18 is fixed to the culture tank 17. Then, the basement membrane is placed on the culture tank 17, and the peristaltic pump 19 is started at the same time, so that the liquid inside the inner tank 312 is sent into the culture tank 17 by the peristaltic pump 19, and the dissection can begin.

[0066] When the liquid level inside the inner tank 312 begins to be consumed, the weight of the inner tank 312 itself begins to decrease. At this time, the elasticity generated by the torsion spring is greater than the weight of the device above the rotating bar 213. The rotating bar 213 will then begin to tilt away from the abutment block 212. When the rotating bar 213 begins to tilt, the rotating plate 221 will also begin to tilt. When the rotating plate 221 tilts, the inner tank 312, the outer tank 222, and the fixed column 322 will also begin to tilt. When the inner tank 312 tilts, the liquid level will not decrease due to the delivery of the peristaltic pump 19. Instead, the outlet pipe will always be submerged in liquid. This is to prevent the static pressure loss that would occur when the liquid level drops as it is continuously consumed. The resulting static pressure loss will cause insufficient initial force for the liquid to enter the outlet pipe 18, slowing down the liquid delivery efficiency. This will prevent the physiological solution from being replenished in time, causing an imbalance in the sample osmotic pressure, and ultimately resulting in sample deformation and unusability.

[0067] When the rotating plate 221 tilts, the moving plate 411 will also tilt. When the moving plate 411 tilts, it will exert a pushing force on the compression strip 425, causing the compression strip 425 to slide inside the connecting post 421. Because the connecting post 421 is filled with liquid, as the compression strip 425 slides away from the moving plate 411, it will begin to compress the spring 423. At this time, the liquid inside the connecting post 421 will begin to impact the flipping plate 422. Because the compression strip 425 is equipped with a second locking block, the flipping plate 422, which was originally in a horizontal state, will return to a vertical position. The flow rate through the compression bar 425 will also decrease. When affected by the spring 423 and the flip plate 422, the sliding speed of the compression bar 425 will begin to slow down. When the sliding speed of the compression bar 425 slows down, the tilting speed of the moving plate 411 will also begin to slow down. The tilting speed of the rotating plate 221 and the rotating bar 213 connected to it will also begin to slow down. In this way, large fluctuations in the liquid surface are prevented when tilting. During the fluctuation, the liquid impacts back and forth in the bottle, forming local turbulence. The shear force generated by the turbulence will cause the static cilia to bend or the basement membrane to be torn.

[0068] When the fixed column 322 begins to tilt, because the fixed column 322 and the counterweight 321 are rotatably connected, the counterweight 321 will remain vertically downward no matter what position the fixed column 322 tilts to. Because the counterweight 321 itself is heavy, and the liquid inside the inner tank 312 is continuously consumed, the inner tank 312 will be affected by the weight of the counterweight 321 itself, and will start to slowly rotate through the rotating disk 311. This will cause the inner tank 312 to start rotating on its own center while tilting. In this way, when the inner tank 312 rotates, it guides the liquid inside the inner tank 312 to form a laminar flow, which counteracts the shaking caused by the inertia of the liquid inside the inner tank 312 when tilting, and prevents the subsequent fluctuation of the liquid due to inertia from causing the hair cells and cilia to bend or break.

[0069] When the rotating plate 221 is tilted, the inner tank 312 also begins to tilt. This causes the residue inside the physiological solution to sink to the end with a smaller tilt angle because the residue is heavier. When the outlet pipe 18 is then extended into the inner tank 312, because the outlet pipe 18 is made of lighter material, it will be located at the end with a larger tilt angle of the rotating plate 221. This keeps the pipe and the residue in different places, preventing the residue from being directly sucked into the outlet pipe 18 when the liquid is drawn in, thus preventing blockage inside the outlet pipe 18.

[0070] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. An integrated microscopic cochlear basilar membrane dissection instrument platform, comprising an external mechanism (1), the external mechanism (1) further comprising a housing (12), a movable microscope (14) rotatably connected to the outer wall of the housing (12), a screen (16) rotatably connected to the outer wall of the housing (12), a slot (13) provided on the outer wall of the housing (12), a culture tank (17) provided on the outer wall of the housing (12), a peristaltic pump (19) fixedly connected to the outer wall of the housing (12), and an outlet pipe (18) fixedly connected to the outer wall of the peristaltic pump (19), characterized in that, Also includes: The fixing mechanism (2) is fixedly connected to the inner wall of the slot (13) to reduce the height difference between the liquid and the outlet when the liquid is drawn. Storage mechanism (3), which is rotatably connected to the inner wall of the fixing mechanism (2) for storing liquid; The deceleration mechanism (4) is fixedly connected to the outer wall of the fixing mechanism (2) and is used to slow down the movement speed of the device.

2. The cochlear basilar membrane dissection instrument platform with integrated microscopic field of view according to claim 1, characterized in that: The fixing mechanism (2) includes: A fixing component (21) is fixedly connected to the inner wall of the slot (13); Motion component (22) is fixedly connected to the outer wall of fixed component (21).

3. The cochlear basilar membrane dissection instrument platform with integrated microscopic field of view according to claim 2, characterized in that: The storage mechanism (3) includes: Storage component (31), which is rotatably connected to the inner wall of motion component (22); Rotating assembly (32) is fixedly connected to the outer wall of storage assembly (31).

4. The cochlear basilar membrane dissection instrument platform with integrated microscopic field of view according to claim 3, characterized in that: The deceleration mechanism (4) includes: A deceleration assembly (41) is fixedly connected to the outer wall of the motion assembly (22); A connecting component (42) is fixedly connected to the inner wall of the slot (13).

5. The cochlear basilar membrane dissection instrument platform with integrated microscopic field of view according to claim 4, characterized in that: The fixing component (21) includes a fixing plate (211) fixedly connected to the inner wall of the slot (13), a stop block (212) fixedly connected to the outer wall of the fixing plate (211), and a rotating strip (213) rotatably connected to the outer wall of the fixing plate (211).

6. The cochlear basilar membrane dissection instrument platform with integrated microscopic field of view according to claim 5, characterized in that: The motion component (22) includes a rotating plate (221) fixedly connected to the outer wall of the rotating bar (213), and an outer tank (222) is fixedly connected to the outer wall of the rotating plate (221).

7. The cochlear basilar membrane dissection instrument platform with integrated microscopic field of view according to claim 6, characterized in that: The storage component (31) includes a rotating disk (311) rotatably connected to the inner wall of the outer tank (222), an inner tank (312) rotatably connected to the outer wall of the rotating disk (311), and a slot (313) is provided on the outer wall of the outer tank (222).

8. The cochlear basilar membrane dissection instrument platform with integrated microscopic field of view according to claim 7, characterized in that: The rotating assembly (32) includes a fixed column (322) fixedly connected to the outer wall of the inner tank (312), and a counterweight (321) is rotatably connected to the outer wall of the fixed column (322).

9. The cochlear basilar membrane dissection instrument platform with integrated microscopic field of view according to claim 6, characterized in that: The deceleration assembly (41) includes a moving plate (411) fixedly connected to the outer wall of the rotating plate (221), and a locking block (412) is fixedly connected to the outer wall of the moving plate (411). When the rotating plate (221) begins to tilt, the moving plate (411) and the locking block (412) will also begin to tilt.

10. The cochlear basilar membrane dissection instrument platform with integrated microscopic field of view according to claim 4, characterized in that: The connecting assembly (42) includes a connecting post (421) fixedly connected to the inner wall of the slot (13), a compression strip (425) slidably connected to the inner wall of the connecting post (421), a fixed frame (424) slidably connected to the outer wall of the compression strip (425), a spring (423) fixedly connected to one end of the compression strip (425) away from the moving plate (411), and a flip plate (422) rotatably connected to the inner wall of the compression strip (425).

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