Positive and negative pressure device for plant in-vitro culture

Abstract

The utility model relates to the technical field of liquid pressure control, and discloses a positive and negative pressure device for plant in-vitro culture, which comprises a gear pump, a liquid storage tank, a bubble remover, an electric control pressure regulating part, a liquid stirring part and a plant fixing part, and the electric control pressure regulating part comprises a first water flow regulating electromagnetic valve, a second water flow regulating electromagnetic valve and a hydraulic pressure gauge. The plant fixing part is provided with a flow guide pipe connecting pipe, a connecting pipe, a metal cable tie A and a metal cable tie B; according to the positive and negative pressure device for plant in-vitro culture, a nutrient solution is stirred through the liquid stirring part, the nutrient solution is conveyed through the gear pump, the water pressure in the flow guide pipe is controlled and adjusted through the electric control pressure adjusting part, and bubbles in the nutrient solution are filtered through the bubble remover. Therefore, the problems that an existing plant in-vitro culture device cannot culture plants with a nutrient solution under the same water pressure in the plants, the survival time of the plants is short, and leaves are prone to withering are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of liquid pressure control, in particular to a positive and negative pressure device for in vitro plant culture. Background Art

[0002] With the development of agricultural technology, in order to make plants grow more luxuriantly, researchers need to accurately understand the nutrients needed for the luxuriant growth of plants. The current mainstream experimental method is soilless cultivation, which uses nutrient solution to culture plants in vitro and conducts experiments by using the controlled variable method on the nutrient solution. Since there is internal water pressure in plants, in order to make the plants survive longer during the experiment, the nutrient solution needs to be cultured with the same water pressure inside the plant. Therefore, applying positive and negative pressure to the nutrient solution during soilless cultivation has become an essential operation.

[0003] In existing in vitro plant culture devices, the nutrient solution cannot be used to culture plants with the same water pressure as inside the plants, resulting in a shorter plant survival time, wilting of leaves, inability to observe plants for a long time, and inaccurate experimental results.

[0004] Therefore, there is an urgent need to invent a positive and negative pressure device for in vitro plant culture to solve the problem that plants cannot be cultured with the same water pressure as inside the plants during the existing in vitro plant culture, resulting in a short plant survival time and easy wilting of leaf morphology. Utility Model Content

[0005] In view of this, the present invention aims at the deficiencies of the prior art and proposes a positive and negative pressure device for in vitro plant culture, aiming to solve the problem that plants cannot be cultured with the same water pressure as inside the plants during the prior art in vitro plant culture.

[0006] The utility model provides a positive and negative pressure device for in vitro culture of plants, comprising:

[0007] A liquid storage tank, used for storing nutrient solution, the liquid storage tank is further provided with a first flow guide pipe and a second flow guide pipe;

[0008] A gear pump is connected to the liquid storage tank through a first flow guide pipe, and the gear pump is used to circulate the nutrient solution in the first flow guide pipe and the second flow guide pipe and provide pressure;

[0009] An electrically controlled voltage regulator connected to the flow guide tube, the electrically controlled voltage regulator being used to monitor the positive and negative pressures of the nutrient solution in the flow guide tube and adjust the positive and negative pressures of the nutrient solution in the flow guide tube;

[0010] A liquid stirring unit is disposed inside the liquid storage tank, and is used to stir the nutrient solution in the liquid storage tank;

[0011] A plant fixing part, used to fix the plant to be cultured in vitro, and the plant fixing part is also used to guide the nutrient solution to the plant;

[0012] The plant fixing part is also provided with a bubble remover, which is used to filter the bubbles in the diverted nutrient solution.

[0013] Furthermore, the liquid storage box comprises:

[0014] A box body, used for storing nutrient solution;

[0015] A liquid inlet is arranged on the top of the box body, the liquid inlet is connected to the box body, and the liquid inlet is used to add the nutrient solution from the outside;

[0016] Two groups of through holes are provided, and the two groups of through holes are arranged oppositely on both sides of the bottom of the box body, and the through holes are used to guide the nutrient solution stored in the box body to the gear pump, and the through holes are also used to guide the nutrient solution pumped out by the gear pump to the box body;

[0017] A dust cover is arranged on the top of the box body, and the dust cover is arranged opposite to the liquid inlet, so that when the dust cover is connected to the box body, the dust cover is used to isolate external dust and impurities;

[0018] A mounting hole is provided on the top of the box body, the mounting hole is communicated with the box body, and the mounting hole is used for mounting the liquid stirring part.

[0019] Furthermore, the electronically controlled voltage regulator comprises:

[0020] a hydraulic pressure gauge, connected to the gear pump, and used to monitor the pressure of the nutrient solution in the guide tube;

[0021] A first water flow regulating solenoid valve and a second water flow regulating solenoid valve are respectively connected to the first flow guiding pipe and the second flow guiding pipe, and the first water flow regulating solenoid valve and the second water flow regulating solenoid valve are respectively used to adjust the flow rate of the nutrient solution in the first flow guiding pipe and the second flow guiding pipe;

[0022] The third flow guide pipe is connected to the hydraulic meter and the gear pump, and the third flow guide pipe is used for guiding the nutrient solution.

[0023] Furthermore, the plant fixing part includes:

[0024] A connecting tube, one end of which is provided with a connecting hole, and the connecting tube is used to fix the plant to be cultivated;

[0025] A guide pipe connecting pipe, one end of which is sleeved on the other end of the connecting pipe, and the other end of the guide pipe connecting pipe is connected to the hydraulic gauge;

[0026] Metal tie A, used to lock and fix the connecting pipe;

[0027] The metal tie B is used to lock and fix the plant to be cultured in vitro.

[0028] Furthermore, the plant fixing part also includes:

[0029] A bubble remover is connected to the guide pipe connecting pipe, and the bubble remover is used to filter bubbles in the nutrient solution in the guide pipe connecting pipe.

[0030] Furthermore, the liquid stirring unit comprises:

[0031] A stirring blade is arranged inside the box, and is used to stir the nutrient solution in the box;

[0032] A stirring rod, arranged inside the box, and used to rotate the stirring blade;

[0033] A motor is arranged on the top of the box body, and the motor is used to rotate the stirring rod.

[0034] Furthermore, the gear pump also includes:

[0035] The first flow guide pipe connection port and the second flow guide pipe connection port are arranged opposite to each other on one side of the gear pump, one end of the first flow guide pipe connection port is connected to the gear pump, the other end of the first flow guide pipe connection port is communicated with the first flow guide pipe, one end of the second flow guide pipe connection port is connected to the gear pump, and the other end of the second flow guide pipe connection port is connected to the third flow guide pipe.

[0036] Furthermore, the gear pump is also provided with a control panel, and the control panel is used to receive control instructions and control the rotation direction of the gear inside the gear pump according to the control instructions.

[0037] Furthermore, the electronically controlled voltage regulator also includes:

[0038] a control unit, electrically connected to the hydraulic pressure meter, the first water flow regulating solenoid valve and the second water flow regulating solenoid valve, respectively, the control unit being configured with a preset water pressure;

[0039] The control unit is used to obtain the real-time water pressure in the guide pipe and adjust the opening or closing of the first water flow regulating solenoid valve and the second water flow regulating solenoid valve.

[0040] Compared with the prior art, the beneficial effects of the utility model are as follows: by setting an electric-controlled voltage-regulating part on the flow-conducting tube for conveying the nutrient solution, controlling the electric-controlled voltage-regulating part through a control unit and controlling the rotation direction of the gear inside the gear pump through a control panel, the water pressure of the nutrient solution in the flow-conducting tube is made the same as the water pressure inside the plant, and by setting a bubble remover on the plant fixing part to filter the bubbles in the nutrient solution. The liquid stirring part is used to stir the nutrient solution in the liquid storage tank to make the nutrients in the nutrient solution more uniform. In addition, the rotation direction of the gear inside the gear pump is the key to controlling the positive and negative water pressure in the flow-conducting tube. The solution of the utility model solves the problem that the existing plant in vitro culture device cannot culture the plant with the nutrient solution at the same water pressure as the plant, resulting in a short plant survival time and easy wilting of the leaf morphology. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 A schematic diagram of the external structure of a positive and negative pressure device for in vitro plant culture provided by an embodiment of the utility model;

[0042] Figure 2 A three-dimensional schematic diagram of the external structure of a positive and negative pressure device for in vitro plant culture provided by an embodiment of the utility model;

[0043] Figure 3 A schematic diagram of the left side of the liquid storage box provided by the embodiment of the utility model;

[0044] Figure 4 for Figure 3 Section view along line AA;

[0045] Figure 5 for Figure 1 A schematic diagram of the enlarged structure at B;

[0046] Figure 6 for Figure 1 Enlarged schematic diagram of the left side of the structure at B.

[0047] Among them: 10, gear pump; 21, dust cover; 22, box body; 23, liquid inlet; 24, first through hole; 25, second through hole; 31, hydraulic gauge; 32, first water flow regulating solenoid valve; 33, second water flow regulating solenoid valve; 40, bubble remover; 51, flow guide pipe connecting pipe; 52, connecting pipe; 53, metal cable tie B; 54, metal cable tie A; 60, liquid agitator; 61, motor; 62, stirring rod; 63, stirring blade; 71, metal belt; 72, rotating head. DETAILED DESCRIPTION

[0048] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0049] In the description of the present application, it should be understood that the terms "center", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0050] The terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, unless otherwise specified, "plurality" means two or more.

[0051] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0052] like Figure 1-6As shown, a positive and negative pressure device for in vitro cultivation of plants includes a gear pump 10, a liquid storage tank 22, a bubble remover 40, an electrically controlled pressure regulating unit 30, a liquid stirring unit 60 and a plant fixing unit 50. A liquid inlet 23 is provided above the liquid storage tank 22 to input external nutrient solution into the liquid storage tank 22. A first through hole 24 and a second through hole 25 are provided on the lower side of the liquid storage tank 22 to circulate the nutrient solution in the liquid storage tank 22 in the guide pipe. A dust cover 21 is provided opposite to the liquid inlet 23 to prevent external impurities from entering the liquid storage tank 22 and causing pollution when the dust cover is connected to the box body. The electrically controlled pressure regulating unit 30 includes a hydraulic gauge, a first water flow regulating solenoid valve 32 and a second water flow regulating solenoid valve 33. The first through hole 24 and the second through hole 25 are respectively connected to the first water flow regulating solenoid valve 32 and the second water flow regulating solenoid valve 33 through the guide pipe, the first water flow regulating solenoid valve 32 is connected to one end of the gear pump 10 through the first guide pipe, the second water flow regulating solenoid valve 33 is connected to the second guide pipe, the second guide pipe is connected to the third guide pipe through the cross pipe, the third guide pipe is connected to the other end of the gear pump 10 through the guide pipe pipe, the hydraulic gauge 31 is connected to the second guide pipe and the third guide pipe through the cross pipe, the hydraulic gauge 31 is used to detect the real-time water pressure in the third guide pipe, adjust the water pressure by the switching degree of the first water flow regulating solenoid valve 32 and the second water flow regulating solenoid valve 33, and adjust the positive and negative water pressure in the guide pipe by the rotation direction of the gear pump 10. The bubble remover 40 is connected to the guide pipe pipe 51, and is used to filter the bubbles in the guide pipe pipe 51. The liquid stirring part 60 includes a motor 61, a stirring rod 62 and a stirring blade 63, which are used to stir the nutrient solution in the liquid storage tank 22. The plant fixing part 50 includes a guide pipe pipe 51, a connecting pipe 52, a metal tie B53 and a metal tie A54. One end of the guide pipe pipe 51 is connected to the hydraulic gauge 31 through a cross pipe, and the other end of the guide pipe pipe 51 is used to install and replace the connecting pipe 52. The connecting pipe 52 is used to fix the cultivated plants. The metal tie A54 is used to lock the connecting pipe 52, and the metal tie B53 is used to lock the cultivated plants.

[0053] Among them, Figure 3 and Figure 4 As shown, the motor 61 is installed on the top of the liquid storage box 22, and the stirring rod 62 and the stirring blade 63 are installed inside the liquid storage box 22 through the mounting hole. After adding new nutrients to the nutrient solution, the motor 61 is turned on, and the stirring rod 62 drives the stirring blade 63 to rotate to stir the nutrient solution evenly.

[0054] Preferably, the stirring rod 62 is a retractable stirring rod, and the liquid level position of the nutrient solution can be detected by a liquid detection device of the stirring rod, thereby controlling the extension and retraction of the stirring rod to immerse the stirring blades in the nutrient solution.

[0055] Among them, Figure 5 and Figure 6As shown, the connecting tube 52 is installed at the guide tube connecting tube 51, and the rotating head 72 on the metal tie B54 at the guide tube connecting tube 51 is rotated clockwise to lock the connecting tube 52 to prevent leakage; the plant to be cultured in vitro is installed at the connecting tube 52, and the rotating head 72 on the metal tie A53 at the connecting tube 52 is rotated clockwise to lock the plant to be cultured in vitro to prevent leakage.

[0056] See also Figure 1 and Figure 2 The utility model provides a positive and negative pressure device for in vitro culture of plants, which adopts a gear pump 10 to transport nutrient solution to control the positive and negative pressure in the device; a water flow regulating solenoid valve is arranged on the two parts of the guide pipe for inlet and outlet of the nutrient solution to control the flow rate and flow velocity of the nutrient solution in the guide pipe, so as to form a flow velocity difference in the two sections of the guide pipe, so as to realize the cultivation of plants with the nutrient solution at the same water pressure as that inside the plants, thereby solving the problem that the existing in vitro culture device for plants cannot cultivate plants with the same water pressure as that inside the plants, resulting in a short survival time of the plants and the wilting of the leaf morphology.

[0057] The utility model provides a positive and negative pressure device for in vitro plant culture that can culture different types of plants. The connecting tube 52 can be replaced to adapt to plant stems of different thicknesses, and then locked with a metal tie A53 to prevent leakage.

[0058] In one embodiment, please refer to Figure 1 and Figure 2 , fix the bamboo at the connecting pipe 52 and lock the metal tie A53, the liquid storage box 22 stores the nutrient solution, open the first water flow regulating solenoid valve 32 and the second water flow regulating solenoid valve 3, and turn on the gear pump 10, filter out the bubbles in the nutrient solution through the bubble remover 40, and the nutrient solution in the device has no pressure. Set the preset pressure to a positive pressure of 30kpa, and the gear pump 10 rotates counterclockwise to reduce the opening degree of the second water flow regulating solenoid valve 33. At this time, the real-time pressure displayed by the hydraulic gauge 31 will increase. After increasing to 30kpa, the opening degree of the second water flow regulating solenoid valve 33 will no longer decrease, so that the pressure is stabilized at 30kpa. Through observation, using a positive pressure of 30kpa on the branches of bamboo, the branches of bamboo can maintain a normal physiological appearance for more than three days, for example, the leaves are normally unfolded and will not curl.

[0059] The control module of the control box is equipped with a plant water potential rhythm simulation program, and the preset water pressure is adjusted by this simulation program. When the culture environment is daytime, the water pressure inside the plant is positive, so the plant water potential rhythm simulation program sets the preset water pressure to a positive value, transmits an electrical signal to the control panel of the gear pump 10, and makes the gears in the gear pump 10 rotate counterclockwise. When the preset water pressure is less than the real-time water pressure, the hydraulic meter 31 transmits an electrical signal to the control box, thereby reducing the opening degree of the second water flow regulating solenoid valve 33 and increasing the opening degree of the first water flow regulating solenoid valve 32 to increase the real-time water pressure in the diversion pipe; when the preset water pressure is greater than the real-time water pressure, the hydraulic meter 31 transmits an electrical signal to the control box, thereby reducing the opening degree of the first water flow regulating solenoid valve 32 and increasing the opening degree of the second water flow regulating solenoid valve 33 to reduce the real-time water pressure in the diversion pipe. When the cultivation environment is dark, the water pressure inside the plant is negative, so the plant water potential rhythm simulation program sets the preset water pressure to a negative value, and transmits an electrical signal to the control panel of the gear pump 10, causing the gears in the gear pump 10 to rotate clockwise. When the preset water pressure is lower than the real-time water pressure, the hydraulic gauge 31 transmits an electrical signal to the control box, thereby reducing the opening degree of the second water flow regulating solenoid valve 33 and increasing the opening degree of the first water flow regulating solenoid valve 32 to increase the real-time water pressure in the diversion pipe; when the preset water pressure is higher than the real-time water pressure, the hydraulic gauge 31 transmits an electrical signal to the control box, thereby reducing the opening degree of the first water flow regulating solenoid valve 32 and increasing the opening degree of the second water flow regulating solenoid valve 33 to reduce the real-time water pressure in the diversion pipe.

[0060] Among them, each component of the electronically controlled voltage regulating unit 30 and the gear pump 10 are electrically connected to the control box through wires.

[0061] Among them, the preset water pressure set in the control box can be positive or negative.

[0062] Working principle: In the present application, firstly, the plant to be cultured in vitro is installed and locked through the connecting tube 52 and the metal buckle 53, the dust cover 21 is opened, and the nutrient solution is added into the liquid storage box 22 through the liquid inlet 23, and then the motor 61 is turned on to stir the nutrient solution, and after stirring evenly, the first water flow regulating solenoid valve 32 and the second water flow regulating solenoid valve 33 are fully opened, and the gear pump 10 is turned on to filter the bubbles in the nutrient solution through the bubble remover 40, and at this time, there is no pressure in the nutrient solution circulating in the device. After the preset pressure is set in the control box, the control box transmits an electrical signal to the electronically controlled pressure regulating unit 30, and increases or decreases the opening degree of the first water flow regulating solenoid valve 32 or the second water flow regulating solenoid valve 33 until the real-time water pressure detected by the hydraulic gauge 31 is stabilized at the preset water pressure value, so as to cultivate the plant with the nutrient solution at the same water pressure inside the plant, so that the plant survives longer and the leaves are not easy to curl.

[0063] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A positive and negative pressure device for in vitro plant culture, characterized in that: include: A liquid storage tank, used for storing nutrient solution, the liquid storage tank is further provided with a first flow guide pipe and a second flow guide pipe; A gear pump is connected to the liquid storage tank through a first flow guide pipe, and the gear pump is used to circulate the nutrient solution in the first flow guide pipe and the second flow guide pipe and provide pressure; An electrically controlled voltage regulator connected to the flow guide tube, the electrically controlled voltage regulator being used to monitor the positive and negative pressures of the nutrient solution in the flow guide tube and adjust the positive and negative pressures of the nutrient solution in the flow guide tube; A liquid stirring unit is disposed inside the liquid storage tank, and is used to stir the nutrient solution in the liquid storage tank; A plant fixing part, used to fix the plant to be cultured in vitro, and the plant fixing part is also used to guide the nutrient solution to the plant; Wherein, the plant fixing part is further provided with a bubble remover, and the bubble remover is used to filter the bubbles in the diverted nutrient solution.

2. The positive and negative pressure device for in vitro plant culture according to claim 1, characterized in that: The liquid storage box comprises: A box body, used for storing nutrient solution; A liquid inlet is arranged on the top of the box body, the liquid inlet is connected to the box body, and the liquid inlet is used to add the nutrient solution from the outside; Two groups of through holes are provided, and the two groups of through holes are arranged oppositely on both sides of the bottom of the box body, and the through holes are used to guide the nutrient solution stored in the box body to the gear pump, and the through holes are also used to guide the nutrient solution pumped out by the gear pump to the box body; A dust cover is arranged on the top of the box body, and the dust cover is arranged opposite to the liquid inlet, so that when the dust cover is connected to the box body, the dust cover is used to isolate external dust and impurities; A mounting hole is provided on the top of the box body, the mounting hole is communicated with the box body, and the mounting hole is used for mounting the liquid stirring part.

3. The positive and negative pressure device for in vitro plant culture according to claim 1, characterized in that: The electronically controlled voltage regulating unit comprises: a hydraulic pressure gauge, connected to the gear pump, and used to monitor the pressure of the nutrient solution in the guide tube; A first water flow regulating solenoid valve and a second water flow regulating solenoid valve are respectively connected to the first flow guiding pipe and the second flow guiding pipe, and the first water flow regulating solenoid valve and the second water flow regulating solenoid valve are respectively used to adjust the flow rate of the nutrient solution in the first flow guiding pipe and the second flow guiding pipe; The third flow guide pipe is connected to the hydraulic meter and the gear pump, and the third flow guide pipe is used for guiding the nutrient solution.

4. The positive and negative pressure device for in vitro plant culture according to claim 3, characterized in that: The plant fixing part comprises: A connecting tube, one end of which is provided with a connecting hole, and the connecting tube is used to fix the plant to be cultivated; A guide pipe connecting pipe, one end of which is sleeved on the other end of the connecting pipe, and the other end of the guide pipe connecting pipe is connected to the hydraulic gauge; Metal tie A, used to lock and fix the connecting pipe; The metal tie B is used to lock and fix the plant to be cultured in vitro.

5. The positive and negative pressure device for in vitro plant culture according to claim 4, characterized in that: The plant fixing part also includes: A bubble remover is connected to the guide pipe connecting pipe, and the bubble remover is used to filter bubbles in the nutrient solution in the guide pipe connecting pipe.

6. The positive and negative pressure device for in vitro plant culture according to claim 1, characterized in that: The liquid stirring unit comprises: A stirring blade is arranged inside the box, and is used to stir the nutrient solution in the box; A stirring rod, arranged inside the box, and used to rotate the stirring blade; A motor is arranged on the top of the box body, and the motor is used to rotate the stirring rod.

7. The positive and negative pressure device for in vitro plant culture according to claim 3, characterized in that: The gear pump also includes: The first flow guide pipe connection port and the second flow guide pipe connection port are arranged opposite to each other on one side of the gear pump, one end of the first flow guide pipe connection port is connected to the gear pump, the other end of the first flow guide pipe connection port is communicated with the first flow guide pipe, one end of the second flow guide pipe connection port is connected to the gear pump, and the other end of the second flow guide pipe connection port is connected to the third flow guide pipe.

8. The positive and negative pressure device for in vitro plant culture according to claim 7, characterized in that: The gear pump is also provided with a control panel, and the control panel is used to receive control instructions and control the rotation direction of the gear inside the gear pump according to the control instructions.

9. The positive and negative pressure device for in vitro plant culture according to claim 3, characterized in that: The electronically controlled voltage regulating unit also includes: a control unit, electrically connected to the hydraulic pressure meter, the first water flow regulating solenoid valve and the second water flow regulating solenoid valve, respectively, the control unit being configured with a preset water pressure; The control unit is used to obtain the real-time water pressure in the guide pipe and adjust the opening or closing of the first water flow regulating solenoid valve and the second water flow regulating solenoid valve.

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