Peptide expression construct and its use

By integrating a destabilization domain and translation separator into nucleic acid molecules, the expression and effectiveness of short peptides as PPI modulators are enhanced, overcoming limitations of biological availability and solubility.

JP2026509481APending Publication Date: 2026-03-19KYOTO PREFECTURAL PUBLIC UNIV CORP
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Patent Information

Application Number
JP2025553704
Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
Priority Date
2023-03-15
Filing Date
2024-03-14
Publication Date
2026-03-19

AI Technical Summary

Technical Problem

Existing short peptides face challenges such as limited biological availability, low solubility, and poor membrane permeability when used as protein-protein interaction (PPI) modulators, and intracellular expression of short peptides is hindered by decreased ribosome binding with shorter coding sequences.

Method used

Incorporation of a destabilization domain (DD) and a translation separator, such as the self-cleaving peptide P2A, into nucleic acid molecules encoding short peptides, facilitating their intracellular expression by acting as a ribosome-binding fragment and stabilizing the target peptide.

Benefits of technology

Enhances the expression of target peptides within cells, improving their effectiveness as PPI modulators by increasing stability and membrane permeability, thereby addressing the limitations of existing peptide therapies.

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Abstract

This disclosure provides a gene transfer construct comprising a destabilization domain (DD) sequence, a translation separator sequence, and a sequence encoding one or more copies of one or more peptides of interest; a nucleic acid encoding the gene transfer construct; the use of a nucleic acid encoding the gene transfer construct to prevent mitochondrial hyperfission and fragmentation; the use of a nucleic acid encoding the gene transfer construct to induce apoptosis in cells, such as cancer cells; and therapeutic applications of a nucleic acid encoding the gene transfer construct, for example, in the treatment of mitochondrial dysfunction and mitochondrial diseases and disorders associated with various types of cancer.
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