A method of preserving Caenorhabditis elegans

Abstract

The invention belongs to the field of biotechnology, and relates to a simple and effective long-term preservation method of Caenorhabditis elegans used in experiments. This method has the disadvantages of the traditional method for preservation experiments with Caenorhabditis elegans, and adopts a solid / liquid mixed medium to cultivate Caenorhabditis elegans to achieve the purpose of long-term (5-7 months) preservation of Caenorhabditis elegans. The outstanding advantages of the present invention are simple preservation operation, simple experimental method, short nematode recovery time and high survival rate.

Description

technical field

[0001] The present invention relates to a method for preserving experimental Caenorhabditis elegans for experiments in the field of biotechnology, especially the -80°C preservation condition in the traditional preservation method cannot be provided in the experimental site, and the activity of nematodes preserved at a general low temperature of 4-15°C is often When it is greatly reduced, it provides a new possibility for long-term preservation of nematodes. Background technique

[0002] Caenorhabditis elegans, belonging to the phylum Nematodes and the class Nematodes, is a non-parasitic nematode with a transparent body and a length of about 1 mm. It is mainly distributed in the soil in temperate regions. The number of cells is small, the function is not complicated, and the body is transparent. It is an ideal model animal for studying biology. Caenorhabditis elegans is widely used in the research of cell development and differentiation, nervous system, aging...

Examples

Embodiment 4

[0026] (4) Selection of Caenorhabditis elegans for preservation in embodiment 4

[0027] Select a petri dish with more L1 and L2 stage Caenorhabditis elegans under starvation state, and pick more than a dozen L1 and L2 stage Caenorhabditis elegans and place them in the gap of NGM solid medium that has been added with LB liquid medium. Carefully seal the Petri dish with parafilm and store it in a 4°C refrigerator for long-term storage.

[0028] (5) Embodiment 5 maintains culture system

[0029] In population ecology, the highest environmental capacity (referred to as "environmental capacity", K value) refers to the maximum number of populations that can be tolerated by a specific environment. The solid / liquid mixed medium ecological system constructed by the method of the present invention can ensure that the reproduction of E.coli OP50 in the S2 area is basically not disturbed by Caenorhabditis elegans and is more suitable for the growth of Caenorhabditis elegans, and it can ...

technical field

[0001] The present invention relates to a method for preserving experimental Caenorhabditis elegans for experiments in the field of biotechnology, especially the -80°C preservation condition in the traditional preservation method cannot be provided in the experimental site, and the activity of nematodes preserved at a general low temperature of 4-15°C is often When it is greatly reduced, it provides a new possibility for long-term preservation of nematodes. Background technique

[0002] Caenorhabditis elegans, belonging to the phylum Nematodes and the class Nematodes, is a non-parasitic nematode with a transparent body and a length of about 1 mm. It is mainly distributed in the soil in temperate regions. The number of cells is small, the function is not complicated, and the body is transparent. It is an ideal model animal for studying biology. Caenorhabditis elegans is widely used in the research of cell development and differentiation, nervous system, aging...

Examples

Embodiment 4

[0026] (4) Selection of Caenorhabditis elegans for preservation in embodiment 4

[0027] Select a petri dish with more L1 and L2 stage Caenorhabditis elegans under starvation state, and pick more than a dozen L1 and L2 stage Caenorhabditis elegans and place them in the gap of NGM solid medium that has been added with LB liquid medium. Carefully seal the Petri dish with parafilm and store it in a 4°C refrigerator for long-term storage.

[0028] (5) Embodiment 5 maintains culture system

[0029] In population ecology, the highest environmental capacity (referred to as "environmental capacity", K value) refers to the maximum number of populations that can be tolerated by a specific environment. The solid / liquid mixed medium ecological system constructed by the method of the present invention can ensure that the reproduction of E.coli OP50 in the S2 area is basically not disturbed by Caenorhabditis elegans and is more suitable for the growth of Caenorhabditis elegans, and it can ...

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