A controllable device for isothermal digestion of tissue samples

Abstract

The present invention discloses a controllable device for the constant temperature digestion process of tissue samples, comprising: a housing, on which a display screen is arranged; a temperature control device, arranged in the housing, for controlling the temperature in the housing, and the temperature control device displays the temperature value on the display screen in real time; a universal shaking device, arranged in the housing, a sample container is arranged on the universal shaking device, and the tissue sample is placed in the sample container, and the universal shaking device drives the sample container to shake; a sample detection device, comprising an infrared emitting device and an infrared receiving device arranged on both sides of the sample container, the infrared light emitted by the infrared emitting device passes through the tissue sample in the sample container and is received by the infrared receiving device, and the infrared receiving device converts the received optical signal into an electrical signal and displays it in real time on the display screen. The present invention controls and monitors the digestion process to the greatest extent, and ensures the best extraction effect.

Description

Technical Field

[0001] The invention relates to the technical field of laboratory equipment design, and in particular to a controllable device for a constant temperature digestion process of a tissue sample. Background Art

[0002] With the development of science and technology, people's understanding of cells is becoming deeper and deeper. In the separation and extraction of primary cells, various digestive enzymes are widely used to digest and decompose biological tissue samples from various sources to obtain specific cells, especially in the extraction of placental stem cells, such as extracting hematopoietic stem cells or placental pluripotent stem cells from the placenta. It is necessary to place the tissue fragments in a constant temperature device in a digestive enzyme solution to decompose and obtain the corresponding target cells;

[0003] The traditional process has the following disadvantages:

[0004] (1) High or low temperature affects enzyme activity and cell activity;

[0005] (2) The rotation speed deviates from the set speed, causing the tissue to be too large or too small, or even directly affecting the cells;

[0006] (3) There is inconsistency in digestion time;

[0007] (4) The degree of digestion is difficult to grasp: Under manual operation, the error is large, resulting in large differences in the digestion process. This process is time-consuming and labor-intensive if it relies solely on manual observation with the naked eye. Summary of the invention

[0008] In order to solve the problems of low filtration efficiency and poor effect of existing mesenchymal stem cells, the present invention provides a controllable device for the constant temperature digestion process of tissue samples, including:

[0009] A housing having a display screen disposed thereon;

[0010] A temperature control device is arranged in the housing and is used to control the temperature in the housing; and the temperature control device displays the temperature value on the display screen in real time;

[0011] A universal shaking table device is arranged in the housing; a sample container is arranged on the universal shaking table device, and the tissue sample is placed in the sample container, and the universal shaking table device drives the sample container to shake;

[0012] The sample detection device comprises an infrared emitting device and an infrared receiving device arranged on both sides of the sample container; the infrared light emitted by the infrared emitting device passes through the tissue sample in the sample container and is received by the infrared receiving device, and the infrared receiving device converts the received optical signal into an electrical signal and displays it in real time on the display screen.

[0013] Preferably, a controller is also included, and the temperature control device, universal shaker device, sample detection device, and display screen are all connected to the controller for controlling start and stop, and the controller receives temperature data from the temperature control device and receives electrical signals from the infrared receiving device and converts them into tissue sample particle size information.

[0014] Preferably, a digital display setting button is provided on the housing, and the digital display setting button is connected to the controller and is used to preset the temperature value of the temperature control device, preset the working state of the universal shaker device, and preset the particle size of the tissue sample detected by the sample detection device.

[0015] Preferably, an alarm indicator light is provided on the housing, and the alarm indicator light is connected to the controller; when the particle size of the tissue sample obtained by the controller reaches or exceeds a preset range, the alarm indicator light is activated.

[0016] Preferably, the temperature control device includes a temperature sensor, a heating device, and a heat dissipation device connected to the controller, and the controller controls the start-up of the heating device or the heat dissipation device according to the temperature value obtained by the temperature sensor.

[0017] Preferably, the universal rocking table device is a rocking table that can rotate 360 ​​degrees.

[0018] Preferably, the universal shaking table device is provided with a replaceable base, and the sample container is a stainless steel container.

[0019] Preferably, the infrared emitting device and the infrared receiving device are arranged at the bottom of the side of the sample container.

[0020] Preferably, the infrared emitting device is disposed on one side of the sample container via a bracket, and the infrared emitting device can be moved up and down and adjusted relative to the bracket.

[0021] Due to the adoption of the above technical solution, the present invention has the following advantages and positive effects compared with the prior art:

[0022] The invention provides a controllable device for the isothermal digestion process of tissue samples. The infrared emitting device of the invention continuously emits infrared rays, which penetrate the sample container and the sample therein and reach the infrared receiving device. The light signal is converted into an electrical signal by receiving the strength of the infrared rays, and the diameter of the sample particles in the sample container is calculated and displayed in real time on the digital display screen. The invention uses the setting of the sample detection device to replace the method of identifying the degree of sample digestion by artificial experience in the prior art, accurately control the digestion process of the sample, reduce human operation errors, control the digestion process to the greatest extent, and ensure the best extraction effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The above and other features and advantages of the present invention will be more clearly understood through the following detailed description in conjunction with the accompanying drawings, in which:

[0024] Figure 1 A schematic diagram of the principle structure of a controllable device for the constant temperature digestion process of tissue samples provided by the present invention. DETAILED DESCRIPTION

[0025] The present invention will be described in more detail below with reference to the accompanying drawings showing embodiments of the present invention. However, the present invention may be implemented in many different forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are set forth in order to achieve a full and complete disclosure and to enable those skilled in the art to fully understand the scope of the present invention. In these drawings, the sizes and relative sizes of layers and regions may be exaggerated for clarity.

[0026] Reference Figure 1 The present invention provides a controllable device for the constant temperature digestion process of tissue samples, comprising a shell 1, a temperature control device, a universal shaking device and a sample detection device; a display screen 2 is arranged on the shell 1; the temperature control device is arranged in the shell 1, and is used to control the temperature in the shell 1, and the temperature control device displays the temperature value on the display screen 2 in real time; the universal shaking device is arranged in the shell 1, and a sample container 8 is arranged on the universal shaking device, and the tissue sample is placed in the sample container 8, and the universal shaking device drives the sample container 8 to shake to achieve mixing of the sample in the sample container 8; the sample detection device comprises an infrared emitting device 13 and an infrared receiving device 9 arranged on both sides of the sample container 8, and the infrared light emitted by the infrared emitting device 13 passes through the tissue sample in the sample container 8 and is then received by the infrared receiving device 9, and the infrared receiving device 9 converts the received optical signal into an electrical signal, converts it into the diameter size of the particle, and displays the average diameter of the particle within 1s on the display screen in real time.

[0027] The infrared emitting device 13 of the present invention continuously emits infrared rays, which penetrate the sample container 8 and the sample therein and reach the infrared receiving device 9. The infrared receiving device 9 continuously receives light signals, and the photoelectric converter in the device can realize the conversion of photoelectric signals to calculate the diameter of the sample particles in the sample container. Specifically, since light propagates in a straight line, after the infrared emitting device 13 emits infrared rays, it will leave a projection on the infrared receiving device 9 due to the obstruction of the particles, and the size of the corresponding particles can be calculated according to the projection size; similarly, since the speed of light is very fast, the infrared receiving device 9 can continuously receive light signals, and the converter displays the average particle diameter within 1S in real time on the digital display screen.

[0028] This device requires that the diameter set on the display screen is the maximum particle diameter allowed after digestion; if the average diameter is lower than the set value for more than 20 consecutive times within 30 seconds, it will be recognized as digestion termination, and the digestion end program of the buzzer alarm device will be activated at the same time;

[0029] The present invention uses the provision of a sample detection device to replace the method of identifying the degree of sample digestion based on manual experience in the prior art, accurately controls the digestion process of the sample, reduces human operation errors, controls the digestion process to the greatest extent, and ensures the best extraction effect.

[0030] Furthermore, when the sample detection device is turned off, the controllable device for the constant temperature digestion process of the tissue sample can be directly used as an ordinary constant temperature shaker, which is suitable for digestion or mixing without requirements on the size of liquid particles.

[0031] In this embodiment, the controllable device of the isothermal digestion process of the tissue sample also includes a controller, and the temperature control device, the universal shaking device, the sample detection device, and the display screen 2 are all connected to the controller for controlling the start and stop, and the controller receives the temperature data on the temperature control device, and receives the electrical signal on the infrared receiving device, which is converted into the particle size information of the tissue sample and displayed in real time on the display screen 2. Among them, the controller can be implemented by a control circuit board or a computer or a control chip and other structures, which are not limited here.

[0032] In this embodiment, the temperature control device includes a temperature sensor 5, a heating device 6, and a heat dissipation device connected to the controller. The controller controls the heating device 6 or the heat dissipation device to start according to the temperature value obtained by the temperature sensor, thereby ensuring the constant temperature effect of the shell.

[0033] Among them, the heating device 6 can be specifically implemented by a heating wire or other structure, and the heat dissipation device can be specifically implemented by a heat dissipation fan 7, 15 arranged on the side wall of the outer shell 1; of course, in other embodiments, the heating device 6 and the heat dissipation device can also be implemented by other structural forms, which are not limited here.

[0034] In this embodiment, the universal shaking table device uses a 360° rotatable shaking table to ensure that the tissue sample is fully mixed.

[0035] Furthermore, a replaceable base 12 is provided on the universal shaking table device 11, and the sample container 8 is fixed on the base 12. The base 12 is a replaceable structure and can have a variety of models to adapt to different sample holding devices, such as centrifuge tubes, blue-capped bottles, and transparent devices such as beakers, thereby greatly broadening the scope of use.

[0036] Among them, the sample container 8 can be fixed on the base 12 by the fixing spring 10, so as to ensure that the sample container is tightly fixed on the base 12 and does not fall off easily; specifically, since the installation spacing of the fixing spring is smaller than the diameter of the container, the container can be fixed by the deformation of the spring, and cooperate with the base 12, so as to fix the sample tightly. Furthermore, if the spring is set to be movable, it can adapt to more container types; of course, in other embodiments, the fixing method of the sample container 8 is not limited to that shown above, and can be adjusted according to specific circumstances, and is not limited here.

[0037] In this embodiment, the infrared emitting device 13 and the infrared receiving device 9 are arranged at the lower end of the side of the sample container 8. Since the tissue sample in the sample container 8 is usually located at the bottom of the sample container 8, the positions of the infrared emitting device 13 and the infrared receiving device 9 are limited to ensure that the infrared rays can smoothly pass through the tissue sample, so as to ensure accurate measurement of the sample particle size.

[0038] In this embodiment, the infrared emitting device 13 is arranged on one side of the sample container 8 through a bracket 14, and the infrared emitting device 13 can be adjusted up and down relative to the bracket 14, thereby realizing the adjustment of the height of the infrared emitting device 13 to adapt to different specifications of containers for holding tissue samples; wherein, the infrared emitting device 13 can realize up and down movement within the range of 1-20cm, and of course, it can also be adjusted according to specific circumstances, which is not limited here.

[0039] In this embodiment, a digital display setting button 16 is provided on the shell, and the digital display setting button 16 is connected to the controller and the display screen 2, and is used to preset the temperature value of the temperature control device, preset the working state of the universal shaker device, and preset the particle size of the tissue sample detected by the sample detection device.

[0040] Specifically, the parameters on the display screen 2 are reasonably set through the digital display setting button 16, for example, the rotation speed of the universal shaker device 11 is set to 1000 rpm, and the acceptable deviation range is 5%; for example, the temperature in the housing 1 is set to 37.0°C, and the acceptable deviation range is 2%, for example, the maximum particle size in the digestive fluid is set to 1 mm.

[0041] In this embodiment, an alarm indicator light is provided on the housing 1, and the alarm indicator light is connected to the controller; the alarm warning light has two modes: abnormal alarm - buzzer alarm does not stop without operation, and digestion end alarm - buzzer alarm for 10s.

[0042] The specific abnormal alarm is: during the operation of the equipment, if the actual speed and temperature deviation exceeds the allowable deviation value, the controller controls the alarm indicator to start the alarm, and the buzzer alarm will continue if no operation is performed.

[0043] The digestion end alarm is specifically: when the particle size of the tissue sample obtained by the controller reaches the preset maximum diameter more than 20 times within 30 seconds, the alarm indicator light starts the alarm.

[0044] In this embodiment, the housing 1 is also provided with a power switch, specifically including an integral switch 3 of the entire device (controlling the display screen 2, the temperature control device, and the cylinder of the universal shaking device) and an infrared switch 4 for controlling the activation of the sample detection device, so that after the infrared switch 4 is independently turned off, the device can also be used as an ordinary constant temperature shaking table, which is suitable for digestion or mixing without specific requirements on the size of liquid particles.

[0045] In this embodiment, the entire device is made of corrosion-resistant and oxidation-resistant material or can be made of stainless steel metal material to extend the service life.

[0046] Those skilled in the art will appreciate that the present invention can be implemented in many other specific forms without departing from its own spirit or scope. Although the implementation of the present invention has been described, it should be understood that the present invention should not be limited to these embodiments, and those skilled in the art can make changes and modifications within the spirit and scope of the present invention as defined by the appended claims.

Claims

1. A controllable device for isothermal digestion of tissue samples, characterized in that: Included are: A housing having a display screen disposed thereon; A temperature control device is arranged in the housing and is used to control the temperature in the housing; and the temperature control device displays the temperature value on the display screen in real time; A universal shaking table device, disposed in the housing; The universal shaking table device is provided with a sample container, the tissue sample is placed in the sample container, and the universal shaking table device drives the sample container to shake; The sample detection device comprises an infrared emitting device and an infrared receiving device arranged on both sides of the sample container; the infrared light emitted by the infrared emitting device passes through the tissue sample in the sample container and is received by the infrared receiving device, the infrared receiving device converts the received optical signal into an electrical signal, calculates the diameter of the sample particles in the sample container and displays it in real time on the display screen, the diameter set on the display screen is the maximum particle diameter allowed after digestion, if the average diameter is lower than the set value for more than 20 times in a row within 30 seconds, it is recognized that the digestion is terminated.

2. The controllable device for isothermal digestion of tissue samples according to claim 1, characterized in that: A controller is also included, and the temperature control device, universal shaker device, sample detection device, and display screen are all connected to the controller for controlling start and stop. The controller receives temperature data from the temperature control device and receives electrical signals from the infrared receiving device and converts them into tissue sample particle size information.

3. The controllable device for isothermal digestion of tissue samples according to claim 2, characterized in that: The housing is provided with a digital display setting button, which is connected to the controller and is used to preset the temperature value of the temperature control device, preset the working state of the universal shaker device, and preset the particle size of the tissue sample detected by the sample detection device.

4. The controllable device for isothermal digestion of tissue samples according to claim 2, characterized in that: The housing is provided with an alarm indicator light, which is connected to the controller; when the particle size of the tissue sample obtained by the controller reaches or exceeds a preset range, the alarm indicator light is activated.

5. The controllable device for isothermal digestion of tissue samples according to claim 2, characterized in that: The temperature control device includes a temperature sensor, a heating device, and a heat dissipation device connected to the controller. The controller controls the heating device or the heat dissipation device to start according to the temperature value obtained by the temperature sensor.

6. The controllable device for isothermal digestion of tissue samples according to claim 1, characterized in that: The universal shaking table device adopts a shaking table that can rotate 360 ​​degrees.

7. The controllable device for isothermal digestion of tissue samples according to claim 1, characterized in that: The universal shaking table device is provided with a replaceable base, and the sample container is a stainless steel container.

8. The controllable device for isothermal digestion of tissue samples according to claim 1, characterized in that: The infrared emitting device and the infrared receiving device are arranged at the bottom of the side of the sample container.

9. The controllable device for isothermal digestion of tissue samples according to claim 1, characterized in that: The infrared emitting device is arranged on one side of the sample container through a bracket, and the infrared emitting device can be moved up and down and adjusted relative to the bracket.

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