Preparation of crocodile nail effective component and application of crocodile nail effective component in resisting oxidation and hepatic fibrosis

Abstract

The invention provides a preparation method of crocodile nail effective components, which belongs to the technical field of biology and comprises the following steps: providing slurry containing water and powdery crocodile nails; performing high-voltage pulse on the slurry to obtain effective components; and recovering the effective components; wherein the effective components contain polysaccharide and protein components; a pharmaceutical composition comprising sophocarpidine alkaloids, crocodile armor effective component and a transfer medium is also prepared. The crocodile armor effective component preparation method provided by the invention can increase slurry fluidity, prevent local high temperature in an electric field from destroying substance activity, and promote transdermal absorption efficiency of effective components; the crocodile armor effective component can be used for preparing a medicinal composition containing the crocodile armor effective component, and also comprises an application of the effective component or the medicinal composition in preparing an antioxidant or anti-hepatic fibrosis medicine.

Description

[0001] This application is a divisional application of Chinese patent application 201910512218.8, submitted on June 13, 2019, entitled "Preparation of effective components of crocodile shell and its application in anti-oxidation and anti-hepatic fibrosis". technical field

[0002] The invention belongs to the field of biotechnology, and in particular relates to the preparation of effective components of crocodile shell and its application in anti-oxidation and anti-hepatic fibrosis. Background technique

[0003] Hepatic fibrosis (Hepatic fibrosis, HF) is a necessary stage for various chronic liver diseases to develop into liver cirrhosis and liver cancer. Hepatic fibrosis is a compensatory product of tissue self-repair after liver injury, the most representative is the excessive deposition of extracellular matrix (ECM) in liver tissue, and fibrous proliferation forms a network of septal barriers. The normal material and energy exchange between liver cells and blood is disrup...

Examples

preparation example Construction

[0039] The preparation method of the effective component of crocodile shell comprises providing a slurry comprising water and powdered crocodile shell; and passing the slurry through high-pressure pulses to burst cells to obtain the effective component; and recovering the effective component;

[0040] The above effective components contain polysaccharides and protein components with a molecular weight of 5-80KD. In the present invention, in order to achieve the purpose of bursting the cells to obtain effective components, the high-voltage pulse operating conditions adopted are: the electric field strength is 20-28kV / cm, the number of pulses is 8-12, and the slurry flow rate is 10-15mL / s . More preferably, the electric field intensity is 20-25 kV / cm, the pulse number is 8-10, and the slurry flow rate is 10-12 mL / s. When the slurry is placed in the electric field, the cell membrane of the crocodile nail cells is punctured by electricity under the action of a strong electric fie...

Embodiment 1

[0057] The preparation method of the medicinal composition containing alligator shell active ingredient, its concrete steps are as follows:

[0058] 1) After the crocodile shell powder is passed through a 300-mesh sieve, double distilled water is added at a ratio of 1:25 (g / mL) to make a slurry, and then the slurry is placed in an electric field with an electric field strength of 20kV / cm, Treat in a high-voltage pulsed electric field with a pulse number of 8, the flow rate of the slurry in the electric field is 10mL / s, collect the treated extract, then centrifuge at 4500r / min for 10min, collect the supernatant, and then pass through dialysis at 4°C. and collecting the dialysate with a molecular weight of 5-80KD, and then freeze-drying the dialysate to obtain the effective component of crocodile carapace;

[0059] 2) Dissolving the effective components of crocodile shell and matrine alkaloids in a phosphate buffer containing 1,2-propanediol, α-pinene and tetramethylglutaric aci...

Embodiment 2

[0063] The preparation method of the medicinal composition containing alligator shell active ingredient, its concrete steps are as follows:

[0064] 1) After the crocodile shell powder is passed through a 400-mesh sieve, double-distilled water is added at a ratio of 1:23 (g / mL) to make a slurry, and then the slurry is placed in an electric field with an electric field strength of 23kV / cm, The pulse number is 10 high-voltage pulsed electric field treatment, the flow rate of the slurry in the electric field is 12mL / s, the treated extract is collected, and then centrifuged at 5000r / min for 10min, the supernatant is collected, and then intercepted by dialysis at 2°C and collecting the dialysate with a molecular weight of 5-80KD, and then freeze-drying the dialysate to obtain the effective component of crocodile carapace;

[0065] 2) Dissolving the effective components of crocodile shell and matrine alkaloids in a phosphate buffer containing 1,2-propanediol, α-pinene and tetramethy...

[0001] This application is a divisional application of Chinese patent application 201910512218.8, submitted on June 13, 2019, entitled "Preparation of effective components of crocodile shell and its application in anti-oxidation and anti-hepatic fibrosis". technical field

[0002] The invention belongs to the field of biotechnology, and in particular relates to the preparation of effective components of crocodile shell and its application in anti-oxidation and anti-hepatic fibrosis. Background technique

[0003] Hepatic fibrosis (Hepatic fibrosis, HF) is a necessary stage for various chronic liver diseases to develop into liver cirrhosis and liver cancer. Hepatic fibrosis is a compensatory product of tissue self-repair after liver injury, the most representative is the excessive deposition of extracellular matrix (ECM) in liver tissue, and fibrous proliferation forms a network of septal barriers. The normal material and energy exchange between liver cells and blood is disrup...

Examples

preparation example Construction

[0039] The preparation method of the effective component of crocodile shell comprises providing a slurry comprising water and powdered crocodile shell; and passing the slurry through high-pressure pulses to burst cells to obtain the effective component; and recovering the effective component;

[0040] The above effective components contain polysaccharides and protein components with a molecular weight of 5-80KD. In the present invention, in order to achieve the purpose of bursting the cells to obtain effective components, the high-voltage pulse operating conditions adopted are: the electric field strength is 20-28kV / cm, the number of pulses is 8-12, and the slurry flow rate is 10-15mL / s . More preferably, the electric field intensity is 20-25 kV / cm, the pulse number is 8-10, and the slurry flow rate is 10-12 mL / s. When the slurry is placed in the electric field, the cell membrane of the crocodile nail cells is punctured by electricity under the action of a strong electric fie...

Embodiment 1

[0057] The preparation method of the medicinal composition containing alligator shell active ingredient, its concrete steps are as follows:

[0058] 1) After the crocodile shell powder is passed through a 300-mesh sieve, double distilled water is added at a ratio of 1:25 (g / mL) to make a slurry, and then the slurry is placed in an electric field with an electric field strength of 20kV / cm, Treat in a high-voltage pulsed electric field with a pulse number of 8, the flow rate of the slurry in the electric field is 10mL / s, collect the treated extract, then centrifuge at 4500r / min for 10min, collect the supernatant, and then pass through dialysis at 4°C. and collecting the dialysate with a molecular weight of 5-80KD, and then freeze-drying the dialysate to obtain the effective component of crocodile carapace;

[0059] 2) Dissolving the effective components of crocodile shell and matrine alkaloids in a phosphate buffer containing 1,2-propanediol, α-pinene and tetramethylglutaric aci...

Embodiment 2

[0063] The preparation method of the medicinal composition containing alligator shell active ingredient, its concrete steps are as follows:

[0064] 1) After the crocodile shell powder is passed through a 400-mesh sieve, double-distilled water is added at a ratio of 1:23 (g / mL) to make a slurry, and then the slurry is placed in an electric field with an electric field strength of 23kV / cm, The pulse number is 10 high-voltage pulsed electric field treatment, the flow rate of the slurry in the electric field is 12mL / s, the treated extract is collected, and then centrifuged at 5000r / min for 10min, the supernatant is collected, and then intercepted by dialysis at 2°C and collecting the dialysate with a molecular weight of 5-80KD, and then freeze-drying the dialysate to obtain the effective component of crocodile carapace;

[0065] 2) Dissolving the effective components of crocodile shell and matrine alkaloids in a phosphate buffer containing 1,2-propanediol, α-pinene and tetramethy...

Images