A method for whole cell catalyzed synthesis of trans-4-hydroxy-l-proline from l-arginine

By utilizing the cascade catalysis of ornithine cyclization deaminase mutant and L-arginine hydroxylase, the production of trans-4-hydroxy-L-proline from L-arginine precursor was achieved, solving the problem of cumbersome existing microbial production processes and providing a new environmentally friendly production method.

CN112877318BActive Publication Date: 2026-06-19JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGNAN UNIV
Filing Date
2021-03-29
Publication Date
2026-06-19

AI Technical Summary

Technical Problem

There are few existing methods for producing trans-4-hydroxy-L-proline using microbial methods, and the catalytic process is cumbersome and consumes a lot of resources.

Method used

Using a "pre-hydroxylation" strategy, L-arginine was used as a precursor to generate trans-4-hydroxy-L-proline through a cascade catalysis of ornithine cyclization deaminase mutant and L-arginine hydroxylase, and whole-cell catalysis was carried out using recombinant Escherichia coli.

Benefits of technology

This provides a new approach for producing Hyp through microbial fermentation with mild reaction conditions, simple original process, and easy control, reducing environmental pollution and making it easy to promote and apply.

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Abstract

This invention discloses a whole-cell catalytic method for the synthesis of trans-4-hydroxy-L-proline from L-arginine, belonging to the field of bioengineering. This invention expresses arginine hydroxylase, arginase, and ornithine cyclization deaminase in *E. coli*, enabling the production of trans-4-Hyp from L-arginine as a substrate in recombinant *E. coli*, providing a new pathway and production strategy for microbial fermentation of Hyp. This whole-cell microbial Hyp production method provided by this invention has advantages such as mild reaction conditions, simple process, and ease of control, while also being environmentally friendly and easy to promote and apply.
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