Fluorescence immunochromatographic kit for simultaneous detection of four nitrofuran metabolites, preparation method and application thereof
By using quantum dot markers to label nitrofuran metabolite antibodies in fluorescent immunochromatography kits, the costly and complex operational problems of detecting nitrofuran drug residues in the prior art are solved, and high-sensitivity on-site rapid detection is achieved.
CN113156112BActive Publication Date: 2025-03-18BOHAI UNIV
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Patent Information
- Application Number
- CN202110446512.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2021-04-25
- Publication Date
- 2025-03-18
- Estimated Expiration
- 2041-04-25
AI Technical Summary
Technical Problem
In the prior art, when detecting nitrofuran drug residues, instrumentation equipment is expensive and complex to operate, and is not suitable for rapid on-site detection, and the sensitivity of colloidal gold immunochromatography technology still needs to be improved.
Method used
The fluorescence immunochromatography kit is used to label four nitrofuran metabolite antibodies by using four quantum dots as markers. The advantages of the quantum dots are long fluorescence lifetime, high quantum yield, and good light stability are improved to improve the detection sensitivity.
Benefits of technology
It realizes the ability to detect four nitrofuran metabolites simultaneously, improves the detection sensitivity, and is suitable for on-site rapid detection.
✦ Generated by Eureka AI based on patent content.
Abstract
The present invention provides a fluorescent immunochromatography kit for simultaneously detecting four nitrofuran metabolites, a preparation method thereof and an application thereof, and belongs to the field of antibiotic detection technology. The kit includes a chromatography test paper and a quantum dot probe; detection lines T1, T2, T3, T4 and a quality control line C are arranged on the nitrocellulose membrane of the chromatography test paper; the detection lines T1, T2, T3, and T4 are sprayed with coated antigens 2-NPAOZ-BSA, 2-NPAHD-BSA, 2-NPSEM-BSA and 2-NPAMOZ-BSA, respectively; the quantum dot probe includes a 2-NPAOZ antibody coupled with a red light quantum dot, a 2-NPAHD antibody coupled with a yellow light quantum dot, a 2-NPSEM antibody coupled with a green light quantum dot, and a 2-NPAMOZ antibody coupled with an orange light quantum dot. The kit of the present invention can simultaneously detect four nitrofuran metabolites with high sensitivity.
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