Polyphosphonic Acids for Improving Recovery and Minimizing System Losses
By reducing the non-specific binding of oligonucleotides by using polyanionic acid competitive binding, the problems of low oligonucleotide recovery and large system losses in the prior art are solved, and efficient analyte recovery and system losses are achieved.
CN114787622BActive Publication Date: 2025-03-25WATERS TECHNOLOGY CORP
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Patent Information
- Application Number
- CN202080083746.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Priority Date
- 2019-12-05
- Filing Date
- 2020-12-04
- Publication Date
- 2025-03-25
- Estimated Expiration
- 2040-12-04
AI Technical Summary
Technical Problem
In the prior art, nonspecific binding of oligonucleotides in chromatography systems, sample vials and solid phase extraction devices leads to low recovery rates, large system losses, and difficult to achieve efficient analysis.
Method used
By using polyanionic acids such as etidronic acid and nitris(methylphosphonic acid), nonspecific binding of oligonucleotides to the system surface is reduced in a competitive manner, improving recovery and reducing system losses.
Benefits of technology
It effectively improves the recovery rate of oligonucleotides, reduces the loss of system analytes, and improves the accuracy and reliability of analysis results.
✦ Generated by Eureka AI based on patent content.
Abstract
The present technology relates to a method for separating a sample containing oligonucleotides. The method includes injecting polyphosphonic acid at a concentration between about 0.01 M and about 1 M into the sample containing oligonucleotides. The method also includes passing the sample and the polyphosphonic acid through a liquid chromatography column, and separating the oligonucleotides.
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