A macrophage digestion solution and a method specifically for digesting macrophages

CN115161281BActive Publication Date: 2026-05-26姜云瀚
View PDF 1 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
姜云瀚
Filing Date
2022-08-09
Publication Date
2026-05-26

AI Technical Summary

Technical Problem

In existing technologies, the digestion process during macrophage culture is sensitive to mechanical stimulation of cells, resulting in high cell differentiation rates and high operational difficulty, which is especially unfriendly to beginners.

Method used

Using a digestion solution containing ethylenediaminetetraacetic acid (EDTA) and ethylene glycol bis(2-aminoethyl ether)tetraacetic acid (EGTA), combined with phosphate buffer and fetal bovine serum, the digestion process reduces mechanical damage by chelating Ca2+ ions in cells. The digestion time can be completed within 1-2 minutes, with high digestibility and reduced differentiation rate.

Benefits of technology

It enables efficient digestion of macrophages under relatively relaxed conditions, reduces the differentiation rate, simplifies the operation, and improves the applicability and stability of the digestion solution.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN115161281B_ABST
    Figure CN115161281B_ABST
Patent Text Reader

Abstract

This invention discloses a macrophage digestion solution and a method specifically for macrophage digestion, comprising the following components: 3-6 parts ethylenediaminetetraacetic acid (EDTA), 4-8 parts ethylene glycol bis(2-aminoethyl ether)tetraacetic acid (EDTA), 8-12 parts fetal bovine serum (FBS), and 90-100 parts phosphate buffer. The method specifically for macrophage digestion includes the following steps: preparing a stock solution of digestion solution, filtering it, and storing it at low temperature for 6 months to obtain the macrophage digestion solution; adding the macrophage digestion solution to a macrophage culture flask; shaking the macrophage culture flask containing the digestion solution to ensure even coverage; adding DMEM culture medium to the settled macrophage culture flask to neutralize the digestion solution, and transferring it to a centrifuge tube; centrifuging the solution in the centrifuge tube at room temperature for 3 minutes; and adding DMEM culture medium to the centrifuge tube after centrifugation to complete the macrophage digestion. This invention achieves high macrophage digestibility and low differentiation rate.
Need to check novelty before this filing date? Find Prior Art