A copper nanocluster-coumarin ratio fluorescence sensor with dual metal ion signal amplification and its application

By using a copper nanocluster-coumarin ratio fluorescence sensor with bimetal ion signal amplification, the catalytic effects of Ce3+ and Fe2+ are used to solve the problem of false positive signals and unvisible detection in hydrogen peroxide and glucose detection in the prior art, achieving high sensitivity and visual detection effects.

CN115290614BActive Publication Date: 2025-03-25WENZHOU MEDICAL UNIV
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Patent Information

Application Number
CN202210054589.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-01-18
Publication Date
2025-03-25
Estimated Expiration
2042-01-18

AI Technical Summary

Technical Problem

The prior art has false positive signals problems in the detection of hydrogen peroxide and glucose, and it is difficult to realize visual detection.

Method used

A copper nanocluster-coumarin ratio fluorescence sensor with bimetal ion signal amplification was used to induce the aggregation induction effect of glutathione-stabilized copper nanoclusters through Ce3+, and under the catalysis of Fe2+, coumarin transforms into a strong fluorescence 7-hydroxycoumarin through the Fenton reaction to construct a ratio fluorescence sensor.

Benefits of technology

High sensitivity detection of hydrogen peroxide and glucose is achieved, and visual detection can be achieved through fluorescence intensity and color changes under irradiation of 365 nm ultraviolet lamps, and the detection range and detection limit are significantly improved.

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Abstract

The present invention belongs to the field of fluorescence sensing technology, and specifically relates to a copper nanocluster-coumarin ratio fluorescence sensor for dual metal ion signal amplification and its application. 3+ The aggregation-inducing effect of glutathione-stabilized copper nanoclusters was induced, which significantly enhanced the fluorescence of copper nanoclusters and significantly improved the fluorescence quenching efficiency of hydrogen peroxide on copper nanoclusters. 2+ Under the catalytic action of Fe 2+ The Fenton reaction with hydrogen peroxide causes non-fluorescent coumarin to be converted into highly fluorescent 7-hydroxycoumarin. A ratiometric fluorescence sensor with copper nanoclusters and coumarin as fluorescent probes was constructed and used for sensitive detection of hydrogen peroxide and glucose. The preparation method of the fluorescence sensor is simple and time-saving. It has high sensitivity when used for the detection of hydrogen peroxide and glucose, and can realize in-situ visual detection of hydrogen peroxide and glucose.
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