Use of a plant growth regulator containing aminoxynate for regulating the growth of ginger

Abstract

The present application relates to a kind of purposes of containing amine fresh ester plant growth regulator for regulating ginger growth, and the effective active ingredient of the plant growth regulator protected by the present application is amine fresh ester and choline chloride, wherein the weight ratio of amine fresh ester and choline chloride is 2:19.Experiments show that the plant growth regulator protected by the present application has good synergistic effect for regulating ginger growth, and once in three strands of ginger (3 seedlings) is applied, and 10-15 days are applied again, has regulating effect on ginger growth, mainly in field performance is that plant height, stem diameter and branch number are increased, can also significantly increase ginger yield, and has improving effect on ginger quality, and has no adverse effect on other non-target organisms.The plant growth regulator of the present application has the advantages of slight toxicity, environmental protection, high efficiency, long effective period, and reducing economic cost.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, specifically relating to a plant growth regulator containing amino esters for regulating ginger growth. The plant growth regulator is composed of amino esters, choline chloride, synergists, and adjuvants. Background Technology

[0002] Aminopropyl ester is a high-energy plant growth regulator with broad-spectrum and groundbreaking effects, discovered by American scientists in the 1990s. Its chemical formula is C. 12 H 25 NO2. Choline chloride can increase the activity of plant peroxidase and nitrate reductase, increase chlorophyll content, accelerate photosynthesis, promote plant cell division and elongation, promote root development, and regulate the balance of nutrients in the plant.

[0003] Choline chloride, with the molecular formula C5H4O 14 ClNO, with a molecular weight of 139.625, is mainly used as a feed additive. In the field of pesticides, it can be absorbed through the stems, leaves, and roots of plants and then quickly transported to the site of action. Its physiological effects can inhibit light absorption in C3 plants, promote root development, and allow photosynthetic products to accumulate in tubers and roots as much as possible, thereby increasing yield and improving quality.

[0004] Ginger growth mainly consists of four stages: germination, seedling, vigorous growth, and rhizome dormancy, each with its own characteristics. As ginger is a warm-season crop, it has strict requirements regarding environment and temperature. Fertilizer and water management, temperature management, and plant immunity all significantly impact ginger yield and quality. Currently, the main pests and diseases affecting ginger are ginger wilt, stem borer, and grubs. The primary control methods are root irrigation and spraying with pesticides. Because underground diseases and pests are difficult to control, farmers often use highly toxic pesticides or mix fertilizers, pesticides, and plant growth regulators to reduce the frequency of pesticide application.

[0005] How to provide a plant growth regulator product that is compatible with various formulations, highly efficient, environmentally friendly, low in toxicity, and has a long-lasting effect, while reducing pesticide residues without compromising efficacy and ensuring safety for consumers, is a technical challenge that urgently needs to be solved by researchers in this field.

[0006] Currently, there are very few legal and compliant compound products on the market that can be used to regulate the growth of ginger. The main products include 18% choline chloride·naphthaleneacetic acid wettable powder, 20% choline chloride·naphthaleneacetic acid aqueous solution, and 8% naphthaleneacetic acid·ethephon soluble solution.

[0007] The applicant has developed a proprietary product and formula by combining aminoethyl esters with choline chloride to regulate ginger growth. This formula effectively promotes photosynthesis in crop leaves and root development, maximizing the accumulation of photosynthetic products in tubers and roots, thereby increasing yield and improving quality. It also enhances crop immunity, prevents premature senescence of stems and leaves, and reduces the probability of pathogen infection, thus achieving disease resistance. Summary of the Invention

[0008] The purpose of this invention is to provide a method for applying plant growth regulators to regulate ginger growth. This method solves the problems of suitable types, concentrations, and timing of plant growth regulators in ginger production, and also addresses the core issues of increasing ginger yield, improving ginger quality, ensuring environmental safety, and minimizing toxicity.

[0009] The technical solution of this invention is:

[0010] A plant growth regulator containing amino esters for regulating ginger growth is characterized in that: the effective active ingredients of the plant growth regulator are amino esters and choline chloride, wherein the weight ratio of amino esters to choline chloride is 2:19.

[0011] Furthermore, the total weight of amino acid esters and choline chloride in the plant growth regulator is 5% to 63%, preferably 10% to 42%, and optimally 21%.

[0012] Furthermore, the plant growth regulator is composed of amino acid esters, choline chloride, synergists, and adjuvants, and is formulated into a wettable powder.

[0013] The synergist is selected from one of polyethoxy-modified silane and a mixture of polyethoxy-modified fatty alcohol or alkoxy-modified polytrisiloxane.

[0014] Furthermore, the plant growth regulator is applied by uniform spraying at an effective ingredient dosage of 126-315 g / ha, once during the three-branch stage of ginger, and then again after an interval of 10-15 days; the preferred effective ingredient dosage is 157.5-189 g / ha for uniform spraying.

[0015] Furthermore, the water consumption for plant growth regulators is 450–600 liters per hectare.

[0016] Compared with the prior art, the present invention has the following advantages:

[0017] (1) Compared with existing technologies, it has a good synergistic effect and is significantly better than the control single agent and blank control;

[0018] (2) The dosage form is environmentally friendly, slightly toxic, safe for the environment, and does not cause pollution. It is also safe for producers, transporters, and users.

[0019] (3) Increase the content of chlorophyll, protein, and nucleic acid in the plant and the photosynthetic rate, increase the activity of peroxidase and nitrate reductase, promote carbon and nitrogen metabolism in the plant, enhance the plant's absorption of water and fertilizer and accumulation of dry matter, thereby improving drought resistance and cold resistance.

[0020] (4) It can enhance crop immunity, improve crop disease and insect resistance, and delay plant aging;

[0021] (5) It has good compatibility and can be used with other pesticides such as fungicides, insecticides and fertilizers without affecting the efficacy of other pesticides;

[0022] (6) It can promote crop metabolism and can be used as an antidote. It has a good repair effect on plant damage caused by excessive pesticide concentration and can reduce the amount of pesticide residue on crops. Detailed Implementation

[0023] Application Example 1: Combined Indoor Toxicity Assay of the Mixture of Amino Acid Ester and Choline Chloride to Regulate Ginger Growth

[0024] 1. Experimental Objective

[0025] Using aminoethyl ester and choline chloride as test agents, and three-branched ginger (Lujiang No. 1) as the test object, the foliar spray method was adopted to determine the regulatory effects of the two agents and their different ratios on ginger growth, and the optimal ratio was screened. The test results are reported as follows.

[0026] 2. Test conditions

[0027] 2.1 Test Target

[0028] Ginger (variety Lujiang No. 1), grown indoors in pots, has reached the three-branch stage.

[0029] 2.2 Cultivation Conditions

[0030] The pot cultivation method was adopted. 450mm × 350mm plastic pots were placed inside enamel dishes, filled with untreated topsoil (4 / 5 full) collected from farmland, dried and sieved. Water was then added directly from the top of the pot to maintain soil moisture at 60%–70% initially. Plump and uniform ginger seeds were selected and evenly placed on the soil surface, covered with 4cm of soil. Soil moisture was maintained by adding water from the bottom after sowing. The pots were placed in an artificial climate chamber with controlled light, temperature, and humidity. When the ginger reached the three-branch stage, ginger with uniform growth was selected as experimental material for pesticide treatment experiments.

[0031] 2.3 Instruments and Equipment

[0032] Electronic balance, pipettes, volumetric flasks, beakers, small quantitative sprayers, plastic basins (450mm×350mm), artificial climate chamber, etc.

[0033] 3 Experimental Design

[0034] 3.1 Reagents

[0035] 3.1.1 Test reagents

[0036] 98% amino ester technical grade;

[0037] 98% choline chloride raw material.

[0038] 3.2 Test Treatment

[0039] 3.2.1 Dosage setting

[0040] A stock solution of 1.0 × 10⁴ mg / L was prepared by using sterile water as a solvent to prepare the test reagents aminoethyl ester and choline chloride technical.

[0041] Based on the individual activity of the two agents and the characteristics of indoor testing, and on the basis of the preliminary test, different combinations of aminoethyl ester and choline chloride were designed using 0.1% Tween 80 aqueous solution, as shown in the table below, with a total of 35 groups designed.

[0042] Using a 0.1% Tween 80 aqueous solution without the reagent as a blank control, indoor bioassays were performed.

[0043] Table 1. Experimental drug dosage design

[0044]

[0045]

[0046] 3.2.2 Experimental Repetition

[0047] Each treatment had 4 replicates, with each replicate containing 20 pots and 1 ginger plant per pot.

[0048] 3.3 Processing Method

[0049] The experiment involved two applications of the drug. One ginger plant was placed in each pot. When the ginger reached the three-branch stage, the plant was treated with the drug, and then treated again after a 10-day interval.

[0050] 4. Test methods

[0051] 4.1 Test Basis and Methods

[0052] The determination of the combined effects of foliar spraying and mixed formulations in the plant growth promotion / inhibition test was conducted in accordance with the "Guidelines for Indoor Bioassay Testing of Pesticides - Plant Growth Regulators" (NY / T2061.2-2011, NY / T 2061.5-2016).

[0053] 4.2 Chemical treatment

[0054] The experiment involved applying the pesticide at the three-branching stage of ginger, with a second application 10 days later. The pesticide was applied until the entire plant's leaves were evenly coated with the solution, without dripping. Spraying was conducted sequentially from low to high doses according to the experimental design, with an aqueous solution containing 0.1% Tween 80 serving as a control.

[0055] 5. Data Survey and Statistical Analysis

[0056] 5.1 Results, Survey Time and Methods

[0057] Sixty days after the second treatment, an absolute value (numerical measurement) survey method was used. Ten pots were randomly selected for each replicate to investigate the number of ginger branches and the yield per plant (the mother ginger was removed, and only the new ginger was retained).

[0058] 5.2 Data Statistical Analysis

[0059] Based on the measurement results, the increase rate of branch number and the increase rate of single tuber weight of ginger under different treatments were calculated. The theoretical growth rate E0 (E0 = X + YX*Y / 100) of each treatment combination was calculated using the Gowing method. Then, it was compared with the measured growth rate (E) to evaluate the combined effect of the two treatments on ginger.

[0060] An E-Eo value greater than 10% indicates a synergistic effect, an E-Eo value less than -10% indicates an antagonistic effect, and an E-Eo value between -10% and 10% indicates an additive effect. The optimal ratio was determined based on factors such as the actual pod number growth rate, the characteristics of the two plant growth regulators, and the balance of the formulation.

[0061] In the formula, X represents the growth rate of branch number and the growth rate of single tuber weight when the dosage of aminoethyl ester is P; Y represents the growth rate of branch number and the growth rate of single tuber weight when the dosage of choline chloride is Q. The experimental results are shown in Table 2.

[0062] 6 Results and Analysis

[0063] Table 2. Combined effects of different ratios of choline chloride and amino esters on ginger growth (Gowing method)

[0064]

[0065] The experimental results showed that aminoethyl ester and choline chloride both had a good effect on increasing the number of branches and the weight of individual tubers in ginger. The E-E0 values ​​of the increase in individual tuber weight in all treatments using aminoethyl ester and choline chloride were positive, showing different trends in efficacy. Among them, the treatment with an aminoethyl ester to choline chloride ratio of 2:19 (20 mg / kg + 190 mg / kg) had the largest E-E0 value of 10.75% on the increase in individual tuber weight, demonstrating a synergistic effect.

[0066] Application Example 2: Formulation Example

[0067] The present invention will be further described below with reference to the embodiments. The percentages in the embodiments are all weight percentages, but the present invention is not limited thereto.

[0068] Example 1: 21% aminoethyl ester·choline chloride wettable powder

[0069] A 21% aminoethyl ester·choline chloride wettable powder was prepared by comprising 2% aminoethyl ester technical grade, 19% choline chloride technical grade, 3% alkoxy-modified polytrisiloxane, 3% alkylnaphthalene sulfonate formaldehyde polymer, 2% polycarboxylate, 4% sodium butylnaphthalene sulfonate, 0.3% anhydrous citric acid, 0.15% organosilicone, 5% silica, and the remainder calcined kaolin.

[0070] Example 2: 21% aminoethyl ester·choline chloride wettable powder

[0071] A 21% aminoethyl ester·choline chloride wettable powder was prepared by mixing 2% aminoethyl ester technical grade, 19% choline chloride technical grade, 4% polyethoxylated modified silane and polyethoxylated modified fatty alcohol mixture, 5% alkyl naphthalene sulfonate, 4% sodium dodecyl sulfate, 1% potassium dihydrogen phosphate, 0.2% fatty alcohol, 20% ammonium sulfate, and the balance being diatomaceous earth.

[0072] Example 3: 10.5% aminoethyl ester·choline chloride wettable powder

[0073] A 10.5% aminoethyl ester·choline chloride wettable powder was prepared by mixing 1% aminoethyl ester technical grade, 9.5% choline chloride technical grade, 5% polyethoxylated modified silane and polyethoxylated modified fatty alcohol mixture, 3% methylnaphthalene sulfonate formaldehyde condensate, 2% polycarboxylate, 2.5% sodium α-alkenyl sulfonate, 1.5% fatty acid sulfate, 0.5% anhydrous citric acid, 0.15% fatty acid, 8% silica, and the balance potassium sulfate.

[0074] Example 4: 42% aminoethyl ester·choline chloride wettable powder

[0075] A 42% aminoethyl ester·choline chloride wettable powder was prepared by comprising 4% aminoethyl ester technical grade, 38% choline chloride technical grade, 4% alkoxy-modified polytrisiloxane, 3% lignin sulfonate, 1% methylnaphthalene sulfonate formaldehyde condensate, 4% sodium butylnaphthalene sulfonate, 1% alkylbenzene sulfonate, 0.3% potassium dihydrogen phosphate, 10% talc, and the balance of calcined kaolin.

[0076] Example 5: 63% aminoethyl ester·choline chloride wettable powder

[0077] A 63% aminoethyl ester·choline chloride wettable powder was prepared by comprising 6% aminoethyl ester technical grade (100% content), 57% choline chloride technical grade (100% content), 4% alkoxy-modified polytrisiloxane, 4% alkylphenol polyoxyethylene ether formaldehyde condensate phosphate, 3% polyoxyethylene polyoxypropylene ether block copolymer, 3% styrene-based phenol formaldehyde resin propylene oxide block polyether, 4% alkyl glycoside, 0.5% glacial acetic acid, 8% silica, and the balance ammonium sulfate.

[0078] Application Example 3: Safety Evaluation Test of 21% Amino Acid Ester·Choline Chloride Wettable Powder (Examples 1-2) on Ginger

[0079] 1.1 Experimental Objective

[0080] A field efficacy evaluation was conducted on the safety of 21% choline chloride·aminoethyl ester wettable powder for ginger.

[0081] 1.2 Test Time

[0082] Experiment start date: July 3, 2019

[0083] Test completion date: October 18, 2019

[0084] 2. Test conditions

[0085] 2.1 Selection of test subjects, crops and varieties

[0086] Test subject: safety

[0087] Experimental crops and varieties: Ginger / White Ginger, Red Claw Ginger, Sichuan Bamboo Root Ginger

[0088] 3 Experimental Design

[0089] 3.1 Dosage and treatment number

[0090] Table 3 Experimental Dosage Settings

[0091]

[0092] 3.2 Usage Method

[0093] Spray, 450L / hectare

[0094] 3.3 Application time and frequency

[0095] Apply the pesticide once when the ginger has three branches (3 seedlings) (July 3, 2019), and apply it again 10-15 days later (July 14), for a total of 2 applications.

[0096] 4.2 Survey methods, timing, and frequency

[0097] 4.2.1 Survey time and number of times

[0098] Three and seven days after application, a survey of phytotoxicity (negative impacts) was conducted. The effects of the pesticide on wild organisms and beneficial insects in the experimental area, as well as on other pests and diseases, were recorded. Plants in each treated area were visually inspected for symptoms of phytotoxicity such as wilting, leaf drop, deformity, yellowing, and discoloration. At ginger harvest (October 18th), the yield of each plot was weighed. Ten ginger plants were randomly selected from each plot, and their plant height, stem diameter, and number of branches were recorded. The yield per plant was also measured.

[0099] 4.2.2 Survey Methods

[0100] Plant height: Measured with a tape measure;

[0101] Stem thickness: Vernier caliper;

[0102] Yield per plant: weighed using a balance.

[0103] Production volume of the community: weighed using a platform scale.

[0104] The results are the average values ​​of each treatment. SPSS statistical software was used for analysis, and the yield results were statistically analyzed using Duncan's New Multiple Range Method (DMRT).

[0105] 4.2.2 Method for calculating drug efficacy

[0106]

[0107] The experimental data were statistically analyzed using SPSS 19 software.

[0108] 5 Results and Analysis

[0109] Table 4. Effects of 21% choline chloride·aminoethyl ester wettable powder on ginger safety.

[0110]

[0111] Table 5. Effects of 21% choline chloride·aminoethyl ester wettable powder on ginger yield.

[0112]

[0113] The experimental results showed that spraying white ginger, red ginger, and Sichuan bamboo root ginger with 21% choline chloride·aminoethyl ester wettable powder at an effective ingredient dosage of 189-756 g / ha did not inhibit their growth and caused no phytotoxicity.

[0114] Application Example 4: Environmental Impact Test of 21% Amino Acid Ester·Choline Chloride Wettable Powder (Examples 1-2)

[0115] Our company commissioned a third-party company to conduct 10 tests on Examples 1-2, including acute oral toxicity tests on bees, acute contact toxicity tests on bees, acute oral toxicity tests on birds, acute toxicity tests on fish, acute toxicity tests on silkworms, acute activity inhibition tests on Daphnia, algal growth inhibition tests, acute toxicity tests on earthworms, acute toxicity tests on the natural enemy Trichogramma wasps, and acute contact toxicity tests on the natural enemy ladybugs. The test results are as follows:

[0116] 1) Acute oral toxicity test of honeybees showed that the toxicity level was slightly toxic.

[0117] 2) Acute contact toxicity test on bees, the toxicity level was slightly toxic.

[0118] 3) Acute oral toxicity test in birds showed a toxicity level of slightly toxic.

[0119] 4) Acute toxicity test on fish, toxicity level is slightly toxic.

[0120] 5) Acute toxicity test of silkworm, the toxicity level is low.

[0121] 6) Acute activity inhibition test of Daphnia showed a toxicity level of slightly toxic.

[0122] 7) Algal growth inhibition test, toxicity level is slightly toxic.

[0123] 8) Acute toxicity test of earthworms, toxicity level is slightly toxic.

[0124] 9) Acute toxicity test of the natural enemy Trichogramma wasp: low risk.

[0125] 10) Acute contact toxicity test of ladybugs (natural enemy): low risk.

[0126] Application Example 5: Acute Toxicology Assays of Examples 1-2

[0127] Our company commissioned a third-party testing agency to conduct acute toxicity tests on Examples 1 and 2. The test results are as follows:

[0128] 1) Acute oral toxicity test in rats showed that the toxicity level was slightly toxic.

[0129] 2) Acute dermal toxicity test in rats showed a toxicity level of slightly toxic.

[0130] 3) Rabbit eye irritation test: no irritation.

[0131] 4) Skin irritation test: No irritation.

[0132] 5) Skin sensitization test. Experimental animal: guinea pig; sensitization intensity: none.

[0133] Application Example 6: Field efficacy experiment of 21% aminoethyl ester·choline chloride wettable powder (Example 1) in regulating ginger growth

[0134] 1. Experimental Objective

[0135] The efficacy of 21% aminoethyl ester·choline chloride wettable powder (Example 1) in regulating ginger growth was evaluated in the field. The experiment screened reasonable field application concentration, timing and method of application, providing a basis for the registration of this product.

[0136] 2 Materials and Methods

[0137] 2.1 Treatment of experimental reagents

[0138] Table 6 Treatment of Test Reagents

[0139]

[0140] 2.2 Experimental subjects and crops

[0141] Experimental subject: Regulated growth.

[0142] Experimental crop: ginger, specifically the Sichuan Zhugen ginger variety.

[0143] 2.3 Experimental site and cultivation conditions

[0144] Test location: Tongliang District, Chongqing.

[0145] 2.4 Processing Methods

[0146] Area of ​​the residential area: 20 square meters; Number of repetitions: 4.

[0147] Apply 450L of pesticide solution per hectare and spray evenly.

[0148] 2.5 Application Time and Method

[0149] Three to seven days after each application, a phytotoxicity (negative impact) survey was conducted. The effects of the pesticide on wild organisms and beneficial insects in the experimental area, as well as on other pests and diseases, were recorded. Ginger plants in each pesticide-treated area were visually inspected for symptoms of phytotoxicity such as wilting, leaf drop, deformity, yellowing, and discoloration.

[0150] At the time of ginger harvest (October 20), five ginger plants were randomly selected from each plot to record plant height, stem diameter, and number of branches, and the yield per plant and the yield of the plot were measured. After harvest, on October 21, the quality of ginger, including soluble protein, crude fiber, and soluble sugar, was measured.

[0151] 2.6 Survey Methods

[0152] Output: Weighed by platform scale

[0153] Coarse fiber: acid-washing method

[0154] Soluble sugars: anthrone method

[0155] Soluble proteins: Coomassie brilliant blue colorimetric method

[0156] The results are the average values ​​of each treatment. SPSS statistical software was used for analysis, and the yield results were statistically analyzed using Duncan's New Multiple Range Method (DMRT).

[0157] 2.7 Calculation and Analysis Methods

[0158]

[0159] 3 Results and Analysis

[0160] Table 7 Effects of 21% aminoethyl ester·choline chloride wettable powder on ginger growth traits

[0161]

[0162] Table 8. Effects of 21% aminoethyl ester·choline chloride wettable powder on ginger yield increase.

[0163]

[0164] Table 9. Effects of 21% aminoethyl ester·choline chloride wettable powder on ginger quality.

[0165]

[0166] The experimental results show that:

[0167] The effects of the tested pesticides on ginger growth and yield are shown in Tables 7 and 8. With increasing pesticide dosage, ginger plant height, stem diameter, and number of branches all increased, with all treatments showing significantly higher plant height, stem diameter, and number of branches than the water control. Compared to the control, except for the treatment with an active ingredient dosage of 126 g / ha, which had similar plant height and stem diameter, all other treatments showed significantly higher plant height and stem diameter. The treatments with active ingredient dosages of 189 g / ha and 315 g / ha showed significantly higher number of branches than the control. Ginger yield increased with increasing pesticide dosage, with plot yields of 62.67 kg, 65.03 kg, 68.42 kg, and 69.11 kg, respectively. All four treatments showed significantly higher yields than the water control. Compared to the control, except for the treatment with an active ingredient dosage of 126 g / ha, which had similar plot yield, the other three treatments showed significantly higher yields than the control. The four treatments promoted ginger growth and significantly increased its yield, with yield increases of 11.13%, 15.32%, 21.32%, and 22.55%, respectively. Except for the 126 g / ha treatment, which had a yield increase comparable to the control, the yield increases of the other three treatments were significantly higher than those of the control.

[0168] The effects of the tested agents on ginger quality are shown in Table 9. Overall, the tested agents improved ginger quality. Compared with the water control group, the soluble protein, crude fiber, and soluble sugar in each treatment were better than those in the water control, indicating that the four concentrations of the tested agents improved the three indicators of soluble protein, crude fiber, and soluble sugar in ginger.

[0169] Application Example 7: Field Efficacy Experiment of 21% Amino Acid Ester·Choline Chloride Wettable Powder (Example 2) in Regulating Ginger Growth

[0170] For specific experimental objectives, experimental basis, experimental reagents and dosages, and calculation methods, please refer to Application Example Six.

[0171] 1. Experimental crop:

[0172] The ginger variety is white ginger;

[0173] Experimental location: Fengdu County, Chongqing

[0174] 2. Application time and frequency

[0175] The experiment involved two applications of the drug. The first application occurred when the ginger was at the three-forked (3-sprout) stage (June 22, 2021). The second application was conducted 15 days later (July 7, 2021).

[0176] 3. Usage capacity

[0177] Water consumption is 450 liters per hectare.

[0178] 4. Survey time and number of times

[0179] Pesticide damage investigation: Observe ginger for pesticide damage 3, 7 and 10 days after each application.

[0180] At harvest time (October 6), the height of ginger plants, stem diameter, and number of branches were investigated, and the yield per plant and the yield per plot were measured to calculate the yield increase rate.

[0181] The quality of ginger, including protein content, crude fiber, and soluble sugar, was determined on October 7th and 8th.

[0182] During the experiment, the effects of the test agent on other non-target organisms were observed.

[0183] 5. Survey Methods

[0184] Plant height, stem diameter, and number of branches: Five sampling points were taken in each plot, and two ginger plants were randomly selected at each point to investigate plant height, stem diameter, and number of branches.

[0185] Yield per plant: Five samples were taken from each plot, and two ginger plants were randomly selected from each sample. The weight of the rhizome of each plant was weighed.

[0186] Yield: Harvest all the ginger from each plot and weigh it using a balance.

[0187] Quality: 25 pieces of ginger of uniform size were randomly selected from each plot, and 5 pieces were divided into 1 part to make a mixed sample. The protein content was determined by Coomassie brilliant blue method, the crude fiber content was determined by acid-base digestion method, and the soluble sugar content was determined by anthrone method.

[0188] 5. Experimental Results

[0189] Table 10 Effects of 21% aminoethyl ester·choline chloride wettable powder on ginger agronomic traits

[0190]

[0191] Table 11 Effect of 21% aminoethyl ester·choline chloride wettable powder on ginger yield

[0192]

[0193] Table 12 Effects of 21% aminoethyl ester·choline chloride wettable powder on ginger quality

[0194]

[0195] The experimental results show that:

[0196] The effects of 21% aminoethyl ester·choline chloride wettable powder on ginger agronomic traits are shown in Table 10. The results showed that after treatment with the agent, the plant height, stem diameter, and number of branches of ginger were significantly higher than those of the water control. Among them, the plant height, stem diameter, and number of branches of the treatment with the effective ingredient of the tested agent at a dosage of 157.5-315 g / ha were significantly higher than those of the two control agents.

[0197] The effects of pesticide treatments on ginger yield are shown in Table 11. The results showed that all pesticide treatments significantly increased ginger yield. Compared to the water control, the yield increases of the four concentrations of the tested pesticides were 8.39%, 12.39%, 20.91%, and 21.64%, respectively. The yield per plant, plot yield, and yield increase rate of the treatments with active ingredient dosages of 157.5–315 g / ha were all significantly higher than those of the two control pesticides.

[0198] The effects of pesticide treatment on ginger quality are shown in Table 12. After pesticide treatment, the protein, vitamin C, and crude fiber content of ginger were not significantly different from those of the water control, indicating that the pesticide application had no adverse effects on ginger quality.

[0199] The above description, in conjunction with specific preferred embodiments, provides a further detailed explanation of the present invention. It should not be construed that the specific implementation of the present invention is limited to these descriptions. For those skilled in the art, various simple deductions or substitutions can be made without departing from the concept of the present invention, and all such modifications and substitutions should be considered within the scope of protection of the present invention.

Claims

1. The use of a plant growth regulator containing amino esters for regulating ginger growth, characterized in that: The effective active ingredients of the plant growth regulator are aminoethyl ester and choline chloride, with the weight ratio of aminoethyl ester to choline chloride being 2:

19.

2. The use according to claim 1, characterized in that: The total weight of amino acid esters and choline chloride in plant growth regulators is 5% to 63%.

3. The use according to claim 2, characterized in that: The total weight of amino acid esters and choline chloride in plant growth regulators is 10%~42%.

4. The use according to claim 3, characterized in that: The total weight of amino acid esters and choline chloride in the plant growth regulator is 21%.

5. The use according to any one of claims 1 to 4, characterized in that: Plant growth regulators are composed of amino acid esters, choline chloride, synergists, and adjuvants, and are formulated into wettable powders.

6. The use according to claim 5, characterized in that: Plant growth regulators should be sprayed evenly at a dosage of 126-315 grams of active ingredient per hectare. Apply once during the three-branch stage of ginger, and repeat once after an interval of 10-15 days.

7. The use according to claim 6, characterized in that: Plant growth regulators should be sprayed evenly at a dosage of 157.5-189 grams of active ingredient per hectare.

8. The use according to claim 6, characterized in that: The water usage for plant growth regulators is 450-600 liters per hectare.

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