A method for extracting tumor cells from urine

By combining EpCAM antibody magnetic beads with immunofluorescence staining, the false positive and false negative problems of traditional urine exfoliative cytology have been solved, enabling efficient separation and identification of tumor cells in urine, which is suitable for early screening and efficacy evaluation of bladder cancer.

CN115655835BActive Publication Date: 2026-07-24THREE GORGES MEDICAL LAB (HUBEI) CO LTD
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Patent Information

Application Number
CN202211177078.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-09-26
Publication Date
2026-07-24
Estimated Expiration
2042-09-26

AI Technical Summary

Technical Problem

Traditional bladder cancer screening methods are difficult to detect small tumors in the early stages. Urine cytology tests have problems with false negatives and false positives, and are not sensitive enough for diagnosing low-grade tumors. They are also heavily affected by impurities, rely on doctors' experience, and are complicated to perform.

Method used

EpCAM antibody magnetic beads are used to selectively identify epithelial cells in urine. Combined with immunofluorescence staining, tumor cells are observed and identified by fluorescence microscopy, simplifying the operation process and improving the degree of automation.

Benefits of technology

It enables efficient separation and identification of tumor cells in urine, reduces the workload of detection, improves detection efficiency and accuracy, and reduces false positives and false negatives, making it suitable for early screening and efficacy evaluation.

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Abstract

The application provides a urine tumor cell extraction method and belongs to the technical field of biological detection. In the application, cells are first separated from a urine sample to prepare a cell precipitate into a cell suspension, then EpCAM antibody magnetic beads are added to the cell suspension, so that tumor cells are combined with the EpCAM antibody magnetic beads and are stably in a cell culture plate under the action of a magnetic field, thereby separating the tumor cells from the urine; finally, tumor cells are identified through a fluorescence in situ hybridization method, and immunotherapy drugs are guided through tumor cell immunofluorescence staining. The method is not affected by red blood cells, white blood cells, dead cell fragments, tubules and crystals and other factors in the urine, and can specifically recognize and capture epithelial cells in the urine through the EpCAM antibody magnetic beads, so that the separation is convenient, the purification degree is high, the cell morphology is complete, and false positives or false negatives caused by cell missed detection can be avoided.
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