Rice blast resistance gene BDR1 and application thereof

By screening and identifying rice receptor-like kinase BDR1, it was found to be a negative regulator that interacts with and phosphorylates MPK3, regulating the jasmonic acid signaling pathway. This solved the problem of easy loss of rice blast resistance genes and achieved a lasting enhancement effect on rice blast resistance.

CN115851782BActive Publication Date: 2026-06-19ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Filing Date
2022-09-28
Publication Date
2026-06-19

AI Technical Summary

Technical Problem

Existing rice blast resistance genes are prone to losing resistance when faced with specific changes in the physiological races of rice blast fungus, making it difficult to breed durable resistant varieties. New target genes are needed to improve the immunity of rice to rice blast fungus.

Method used

Rice receptor-like kinase BDR1 was screened and identified, and found to be a negative regulator. Silencing or knocking out BDR1 significantly improved rice blast resistance. Protein-protein interaction and kinase activity assays revealed that BDR1 interacts with and phosphorylates MPK3, regulating the jasmonic acid signaling pathway to negatively mediate rice blast resistance.

Benefits of technology

The BDR1-MPK3 molecular module significantly enhances rice resistance to rice blast by influencing JA-related defense responses. Silencing or knocking out the BDR1 gene enhances rice resistance to rice blast fungus, while overexpression of BDR1 reduces resistance. It negatively mediates rice blast resistance by regulating the JA signaling pathway.

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Abstract

This invention provides a rice blast resistance gene BDR1 and its application. The nucleotide sequence of the rice blast resistance gene BDR1 is SEQ ID NO.1. The amino acid sequence of the protein encoded by the rice blast resistance gene BDR1 is SEQ ID NO.2. The beneficial effects of this invention are: BDR1 expression increases rapidly under M. oryzae infection; silencing or knocking out BDR1 significantly improves rice blast resistance in rice varieties. Through protein-protein interaction and kinase activity assays, we found that BDR1 directly interacts with and phosphorylates MPK3. Knockout of BDR1 reduces the phosphorylation level of MPK3 induced by M. oryzae. Transcriptome analysis showed that the jasmonic acid (JA) signaling pathway induced by M. oryzae is negatively regulated by BDR1 and MPK3. The BDR1-MPK3 molecular module negatively mediates rice blast resistance by affecting JA-related defense responses.
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