Mining of SNPs in 5a genome sequence of wheat kernel weight gene and development and application of kasp molecular marker

By mining closely linked SNP variant sites on wheat chromosome 5A and developing KASP molecular markers, the problem of rapidly and accurately identifying grain traits in wheat grain weight research has been solved, thus improving the efficiency of wheat breeding.

CN115927557BActive Publication Date: 2026-07-07INSTITUTE OF CROP SCIENCE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INSTITUTE OF CROP SCIENCE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Filing Date
2022-07-21
Publication Date
2026-07-07

AI Technical Summary

Technical Problem

In wheat grain weight research, due to the large and complex genome structure of wheat, there are few examples of directly isolating individual grain weight genes, and existing technologies are difficult to effectively use molecular biology methods for rapid and accurate identification of grain traits.

Method used

By mining two closely linked SNP variant sites on wheat chromosome 5A, KASP molecular markers were developed. Competitive allele-specific PCR technology was used for genotyping to rapidly identify wheat grain traits, including grain weight, grain length, grain width, and grain thickness.

Benefits of technology

It enables rapid and accurate identification of grain traits, improves the efficiency of molecular marker-assisted selection and breeding of wheat grain weight, and has significant breeding application prospects.

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Abstract

The application discloses mining of a wheat kernel weight gene 5A genomic sequence SNP and development and application of a KASP molecular marker. The application provides a method for identifying or assisting in identifying a wheat kernel trait, and comprises the following steps: detecting a genotype of a SNP-1126 site in genomic DNA of a wheat to be detected, and determining whether the genotype is an AA genotype, a GG genotype or an AG genotype; the kernel trait of the AA genotype wheat is better than that of the GG genotype wheat. The application also provides a method for identifying or assisting in identifying a wheat kernel trait, and comprises the following steps: detecting a genotype of a SNP-764 site in genomic DNA of a wheat to be detected, and determining whether the genotype is a TT genotype, a CC genotype or a TC genotype; the kernel trait of the TT genotype wheat is better than that of the CC genotype wheat. The application has a good application prospect in molecular marker assisted selection and molecular design breeding for improving the wheat kernel weight.
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