Primer probe combination, kit, method and application for detecting monkeypox virus

By using loop-mediated isothermal amplification technology and a combination of dual-target primer probes in monkeypox virus detection, the problem of long detection time and insufficient accuracy in the prior art is solved, and fast and accurate monkeypox virus detection is achieved, which is suitable for grassroots applications.

CN115948612BActive Publication Date: 2025-03-28SHANGHAI PUBLIC HEALTH CLINICAL CENT
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Patent Information

Application Number
CN202211270444.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-10-18
Publication Date
2025-03-28
Estimated Expiration
2042-10-18

AI Technical Summary

Technical Problem

The prior art is difficult to detect monkeypox virus quickly and accurately, and traditional biological differential diagnosis methods have problems such as biosafety risks and long detection time.

Method used

Kits and methods for detecting the OPG002 gene and ATI gene of monkeypox virus were designed using loop-mediated isothermal amplification technology (LAMP) combined with a dual-target primer probe combination. This method achieves rapid amplification and detection of nucleic acids under constant temperature conditions by introducing specific primers and probes.

Benefits of technology

The monkeypox virus detection is achieved within 40 minutes, which improves the speed and accuracy of the detection, is suitable for rapid diagnosis at the grassroots level, and reduces biosafety risks.

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Abstract

The present invention relates to a dual-target primer-probe combination, a kit, a method and an application for detecting monkeypox virus, and belongs to the field of biomedical technology. The present invention provides a diagnostic reagent for detecting monkeypox virus, and the reagent is used to detect the OPG002 gene and the ATI gene of the monkeypox virus; the reagent includes primers and probes with nucleotide sequences such as SEQ ID No.1 to SEQ ID No.52. The bases at both ends of the probe are respectively labeled with a fluorescent group and a quenching group. The present invention provides a LAMP kit for detecting monkeypox virus, and an application of a primer combination in the preparation of a monkeypox virus detection kit; the present invention obtains a kit and a diagnostic method for quickly and accurately diagnosing monkeypox virus; the provided kit is simple and quick to use, and is suitable for rapid diagnosis at the grassroots level.
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