Aerosol filter and nucleic acid extraction cartridge

Abstract

An aerosol filter and a nucleic acid extraction cartridge, the nucleic acid extraction cartridge comprising a liquid storage module, an extraction module, a communication module and the aerosol filter. The communication module connects the liquid storage module and the extraction module, and is connected with an external air pressure driving device to drive fluid transmission in the nucleic acid extraction cartridge. The aerosol filter is arranged between the communication module and the external air pressure driving device, and comprises a cover, a base, a filter membrane and a silica gel pad. The filter membrane is arranged in a filter membrane bearing groove of the base, and the silica gel pad is arranged between the cover and the base to achieve air tightness of a filter cavity defined by the cover and the base. The cover and the base have a plurality of filter membrane support structures arranged correspondingly and respectively abutting against two opposite surfaces of the filter membrane to fix the filter membrane in the filter membrane bearing groove of the base and jointly support the filter membrane from being deformed due to air pressure during filtration.

Description

Technical Field

[0001] This case relates to an aerosol filter, particularly an aerosol filter suitable for nucleic acid extraction cartridges. Background Technology

[0002] Until effective treatments are available, clinical medicine has been seeking solutions for point-of-care testing (POCT) for suspected cases to enable early disease detection and prevention. While molecular diagnostics can detect target pathogens with excellent sensitivity and accuracy, conventional methods require highly trained technicians, expensive laboratory equipment, and complex procedures. Therefore, traditional molecular diagnostic methods cannot meet the demands for targeted and real-time disease diagnosis.

[0003] In recent years, the development of "chip labs" has gradually matured. Its core concept is to miniaturize and integrate the functions of different testing instruments and equipment onto a single testing platform to achieve the goal of point-of-care testing. Key technologies of this platform include: constructing consumable cartridges with "nucleic acid extraction and purification" and "quantitative micro-sampling and dispensing" functions through fluid control and microfluidic technology, simplifying the originally complex operating procedures; and building a fully functional portable testing instrument through the integration of optics, mechanisms, electronic control, and cloud data. This miniaturizes the key functions of multiple large devices into a single desktop device to meet the needs of on-site testing and report analysis. This fully automated nucleic acid testing platform can be applied to infectious disease testing, not only overcoming the current limitation that nucleic acid testing can only be performed in specific medical testing centers or laboratories, but also reducing complex operating procedures and errors that may be caused by human interpretation, providing frontline personnel (such as nurses and physician assistants) with rapid and accurate clinical diagnosis of infectious diseases.

[0004] For pneumatically driven nucleic acid extraction cartridges, regardless of whether they are pump-driven or piston-driven, the cartridge body design must include air inlet and outlet ports and flow channel structures. The corresponding analytical device body must then have a fluid control module corresponding to this cartridge structure, including a pump, solenoid valve, piston, and related control electronic circuit modules. However, during the pump or piston-driven process, nucleic acid extraction reagents inevitably generate aerosols, which are discharged into the analytical device's cavity through the pump, piston, and fluid design. Furthermore, nucleic acid extraction reagents mostly use ethanol as a solvent; during the extraction process, volatile organic gases emitted from ethanol are also easily discharged into the analytical device's cavity along with the pump, piston, and fluid design. While aerosols and volatile organic gases are not likely to cause significant damage to the analytical device, nucleic acid extraction reagents are mostly high-salt solutions. If these salts are carried into the analytical device through aerosols or volatile gases, they can easily cause accumulation, blockage, or corrosion damage to the analytical device's pipes, pumps, or electronic control components due to salt residue. The salt components are mainly contributed by the lysis buffer and wash buffer, which contain sodium hydroxide (NaOH) and guanidine hydrochloride (GuHCl), and will individually dissociate in solution and contribute Na. + OH - C(NH2) 3+ Cl - Due to the airflow control during the extraction process, the aforementioned salt ions in the plasma, ethanol, or water solution can be carried by ethanol or water vapor aerosols (a dispersion system where solid or liquid particles are stably suspended in a gaseous medium) or vapors to upstream pipelines or electrical control components, forming salt deposits that can cause pipeline blockages or corrosion and damage to electrical control components. Therefore, how to avoid the aforementioned salt deposits causing pipeline blockages or corrosion and damage to electrical control components is an urgent problem to be solved. Summary of the Invention

[0005] The purpose of this invention is to provide an aerosol filter suitable for nucleic acid extraction cartridges, which filters out aerosols carrying salt ions generated by airflow during nucleic acid extraction, thereby preventing upstream equipment pipes or electrical control components from being blocked or corroded and damaged by salt substances.

[0006] To achieve the above objectives, this invention provides an aerosol filter suitable for a nucleic acid extraction cartridge. The nucleic acid extraction cartridge includes a liquid storage module, an extraction module, and a connecting module. The connecting module connects the liquid storage module and the extraction module and is connected to an external pneumatic drive device to drive fluid transport within the nucleic acid extraction cartridge. The aerosol filter is disposed between the connecting module and the external pneumatic drive device and includes: a top cover and a base, the top cover and base defining a filter cavity; a filter membrane disposed in a filter membrane support groove in the base; and a silicone pad disposed between the top cover and the base, configured to ensure the filter cavity defined by the top cover and the base is airtight. The top cover and the base have multiple correspondingly disposed filter membrane support structures, each correspondingly abutting against two opposite surfaces of the filter membrane to fix the filter membrane in the filter membrane support groove of the base and jointly support the filter membrane to prevent deformation due to air pressure during filtration.

[0007] In one embodiment, the filter membrane is a polytetrafluoroethylene (PTFE) membrane.

[0008] In one embodiment, the pore size of the filter membrane is 0.02–0.5 μm.

[0009] In one embodiment, the aerosol filter further includes another silicone pad disposed on the top surface of the cover and mounted on a gas conduit connected to an external pneumatic drive device.

[0010] In one embodiment, the filter membrane carrier groove includes a filter membrane attachment area, which is a protrusion extending inward from the periphery of the filter membrane carrier groove.

[0011] In one embodiment, the multiple filter membrane support structures of the base are multiple protrusions extending upward from the bottom surface of the filter membrane carrier groove.

[0012] In one embodiment, the multiple filter membrane support structures of the top cover are multiple protrusions extending downward from the bottom surface of the top cover.

[0013] In one embodiment, the silicone pad includes a slot and a peripheral wall. The slot is configured to roughly correspond to the filter membrane support groove, and the peripheral wall is disposed at the periphery of the slot and protrudes from the upper and lower surfaces of the silicone pad. The top surface of the peripheral wall abuts against the bottom surface of the top cover, and the bottom surface of the peripheral wall abuts against the filter membrane.

[0014] In one embodiment, the top cover includes a frame that extends downward from the bottom surface of the top cover and abuts against the silicone pad, and the inner surface of the frame is in contact with the outer surface of the peripheral wall of the silicone pad.

[0015] In one embodiment, the base includes a plurality of ultrasonic welding ribs, which are a plurality of protrusions extending upward from the top surface of the base.

[0016] In one embodiment, the top cover, the base, and the silicone pad each have a plurality of correspondingly provided pneumatic openings to collectively define a plurality of gas channels, and at least one of the plurality of gas channels passes through a filter membrane in a filter membrane carrier groove.

[0017] To achieve the above objectives, this application also provides a nucleic acid extraction cartridge, including a liquid storage module, an extraction module, a connecting module, and an aerosol filter. The connecting module connects the liquid storage module and the extraction module, and is connected to an external pneumatic drive device to drive fluid transport within the nucleic acid extraction cartridge. The aerosol filter is disposed between the connecting module and the external pneumatic drive device, and includes: a top cover and a base, the top cover and the base defining a filter cavity; a filter membrane disposed in a filter membrane support groove of the base; and a silicone pad disposed between the top cover and the base, configured to make the filter cavity defined by the top cover and the base airtight. The top cover and the base have a plurality of correspondingly disposed filter membrane support structures, which respectively abut against two opposite sides of the filter membrane to fix the filter membrane in the filter membrane support groove of the base, and jointly support the filter membrane to prevent it from deforming due to air pressure during filtration. Attached Figure Description

[0018] Figure 1 This diagram shows the assembly schematic of the nucleic acid extraction cartridge in this case;

[0019] Figure 2 This image shows an exploded view of the nucleic acid extraction cartridge used in this case.

[0020] Figure 3 Showing an exploded view of the aerosol filter in this case;

[0021] Figure 4 This diagram shows the structural correspondence between the filter membrane and the base of the aerosol filter in this case.

[0022] Figure 5 Showing the bottom structure of the top cover of the aerosol filter in this case;

[0023] Figure 6 This diagram shows a cross-sectional view of the aerosol filter and its filtration mechanism.

[0024] Figure 7 This section presents an analysis of the salt filtration efficiency of the aerosol filter used in this case.

[0025] [Symbol Explanation]

[0026] C: Nucleic Acid Extraction Cartridge

[0027] 1: Liquid Storage Module

[0028] 2: Extraction Module

[0029] 3: Connectivity Module

[0030] 4: Sampling Module

[0031] 41: Sampling tube

[0032] 5: Aerosol Filter

[0033] 51: Top Cover

[0034] 511: First aerodynamic opening

[0035] 512: Filter membrane support structure

[0036] 513: Frame

[0037] 52: Base

[0038] 521: Filter membrane carrier tank

[0039] 522, 522A: Second pneumatic opening

[0040] 523: Filter membrane attachment area

[0041] 524: Filter membrane support structure

[0042] 525: Ultrasonic welding rib

[0043] 53: Filter membrane

[0044] 531: Adhesive

[0045] 532: Extension

[0046] 54: Silicone pad

[0047] 541: Third pneumatic opening

[0048] 542: Grooving

[0049] 543: Zhou Bi

[0050] 544: Opening

[0051] 55: Silicone pad

[0052] 551: Fourth pneumatic opening Detailed Implementation

[0053] Some embodiments embodying the features and advantages of this invention will be described in detail in the following description. It should be understood that this invention can have various variations in different forms, all of which do not depart from the scope of this invention, and the descriptions and drawings herein are for illustrative purposes only and not intended to limit this invention.

[0054] This case mainly provides an aerosol filter and a suitable nucleic acid extraction cartridge. Figure 1 This diagram shows the assembly schematic of the nucleic acid extraction cartridge in this case. Figure 2 This image shows an exploded view of the nucleic acid extraction cartridge used in this case. Figure 1 and Figure 2 As shown, the nucleic acid extraction cartridge C includes at least a reservoir module 1, an extraction module 2, and a connecting module 3. The reservoir module 1 has multiple tanks for storing various reaction reagents or buffer solutions. The extraction module 2 has multiple chambers (e.g., reaction chamber, collection chamber, waste chamber, etc.) and flow channels for performing the nucleic acid extraction process. The connecting module 3 connects the reservoir module 1 and the extraction module 2 and is connected to an external pneumatic drive device (not shown), which drives fluid transport within the nucleic acid extraction cartridge C. For example, the external pneumatic drive device can be a pump drive device or a piston drive device, providing a gas pressure source and using solenoid valves to control the switching of gas input to each tank or chamber to drive fluid flow between the tanks or chambers.

[0055] In one embodiment, the nucleic acid extraction cartridge C further includes a sampling module 4, which is connected to the extraction module 2 and is configured to collect and dispense the extracted nucleic acid solution into the sampling tube 41 to facilitate subsequent nucleic acid amplification and detection. In one embodiment, the sampling tube 41 may be an Eppendorf tube, but is not limited thereto.

[0056] According to the concept of this case, the nucleic acid extraction cartridge C also includes an aerosol filter 5, which is located between the connecting module 3 and the external pneumatic drive device. Figure 3 This shows an exploded view of the aerosol filter in this case. Figure 4 This diagram shows the structural correspondence between the filter membrane and the base of the aerosol filter. Figure 5 Showing the bottom structure of the top cover of the aerosol filter. Figure 6 This shows a cross-sectional view of an aerosol filter and its filtration mechanism. For example... Figures 3 to 6 As shown, the aerosol filter 5 includes a top cover 51, a base 52, a filter membrane 53, and a silicone pad 54. The top cover 51 and the base 52 fit together to define a filter cavity. The top cover 51 is connected to an external pneumatic drive device and has multiple first pneumatic openings 511. The base 52 is connected to the communication module 3 and has a filter membrane support groove 521 and multiple second pneumatic openings 522 corresponding to the multiple first pneumatic openings 511. The filter membrane 53 is disposed in the filter membrane support groove 521 of the base 52. The silicone pad 54 is disposed between the top cover 51 and the base 52 and has multiple third pneumatic openings 541 corresponding to the multiple first pneumatic openings 511, configured to make the filter cavity defined by the top cover 51 and the base 52 airtight.

[0057] According to the concept of this invention, the filter membrane 53 filters water molecules in the airflow through its porosity, thereby filtering out salts and protecting the equipment from blockage or damage caused by salts. The filter membrane 53 can be made of different materials depending on the characteristics of the reagent or the size of the molecules to be filtered, and is preferably a hydrophobic filter membrane. In one embodiment, the filter membrane 53 is, for example, a polytetrafluoroethylene (PTFE) membrane with a pore size of 0.02–0.5 μm, preferably 0.1–0.3 μm. For example, the filter membrane 53 can be a PTFE membrane with a pore size of 0.22 μm. Of course, the material of the filter membrane 53 is not limited to polytetrafluoroethylene; it can also be nylon, polyvinylidene fluoride (PVDF), mixed cellulose ester (MCE), polyethersulfone (PES), etc. In one embodiment, the area of ​​the filter membrane 53 is 100–300 mm². 2 However, this is not the only limit.

[0058] In one embodiment, such as Figure 3 As shown, the aerosol filter 5 includes another silicone pad 55 disposed on the top surface of the upper cover 51 and having a plurality of fourth pneumatic openings 551 corresponding to a plurality of first pneumatic openings 511. It is structured within a gas pipeline connecting to an external pneumatic drive device, ensuring the gas pipeline is airtight. Specifically, the aerosol filter 5 engages with the connecting tube or needle of the external pneumatic drive device via the silicone pad 55 to provide an airtight engagement environment, facilitating the outflow and inflow of the drive airflow.

[0059] As mentioned above, the plurality of first pneumatic openings 511 of the top cover 51, the plurality of second pneumatic openings 522 of the base 52, the plurality of third pneumatic openings 541 of the silicone pad 54, and the plurality of fourth pneumatic openings 551 of the silicone pad 55 are arranged in a corresponding manner to define a plurality of gas channels and connect to the gas channels of an external pneumatic driving device, thereby driving the fluid transport within the nucleic acid extraction cartridge C.

[0060] In one embodiment, such as Figure 4As shown, the filter membrane support groove 521 of the base 52 has a shape and size that is approximately the same as that of the filter membrane 53, and the filter membrane support groove 521 includes a filter membrane attachment area 523 and multiple filter membrane support structures 524. The filter membrane attachment area 523 is disposed on the inner edge of the filter membrane support groove 521, and is a protrusion extending inward from the periphery of the filter membrane support groove 521, and is disposed corresponding to the periphery of the filter membrane 53. When the bottom surface of the periphery of the filter membrane 53 is coated with adhesive (e.g., acrylic adhesive) 531, it can be attached to the filter membrane attachment area 523. The filter membrane support structures 524 are disposed inside the filter membrane support groove 521, and are multiple protrusions extending upward from the bottom surface of the filter membrane support groove 521, used to support the filter membrane 53 to prevent it from deforming due to air pressure during the filtration process, thereby blocking the airflow channel. The top surface of the filter membrane attachment area 523 and the top surface of the filter membrane support structure 524 are approximately on the same horizontal plane to jointly support the filter membrane 53 on the same plane. In other words, the filter membrane attachment area 523 also provides a support structure, and by combining with the upper cover 51 and the silicone pad 54, it achieves the function of fixing the filter membrane 53 (e.g., Figure 6 (As shown).

[0061] In one embodiment, such as Figure 5 As shown, the upper cover 51 also includes a filter membrane support structure 512, which is a plurality of protrusions extending downward from the bottom surface of the upper cover 51 in the area corresponding to the filter membrane support groove 521, and is provided corresponding to the filter membrane support structure 524 of the base 52. When the upper cover 51 and the base 52 are fitted together, the filter membrane support structure 512 of the upper cover 51 and the filter membrane support structure 524 of the base 52 respectively abut against the two opposite surfaces of the filter membrane 53 (e.g., ...). Figure 6 As shown), the filter membrane 53 is fixed in the filter membrane support groove 521 of the base 52, and together they support the filter membrane 53 so that it does not deform due to air pressure during the filtration process, thereby blocking the airflow channel.

[0062] In one embodiment, such as Figure 3 As shown, the silicone pad 54 includes a groove 542 and a peripheral wall 543. The groove 542 is configured to roughly correspond to the filter membrane support groove 521, and the peripheral wall 543 is disposed at the periphery of the groove 542 and protrudes from the upper and lower surfaces of the silicone pad 54. The top surface of the peripheral wall 543 abuts against the bottom surface of the upper cover 51, and the bottom surface of the peripheral wall 543 abuts against the periphery of the filter membrane 53 and the filter membrane attachment area 523 (e.g., ...). Figure 6 (As shown). Therefore, when the top cover 51 and the base 52 are fitted together, applying pressure to the top cover 51 will fix the filter membrane 53 to the filter membrane attachment area 523 of the base 52 through the silicone pad 54.

[0063] In one embodiment, the number of filter membranes 53 in the aerosol filter 5 is not limited to one, but can also be multiple. When there are multiple filter membranes 53, the number of filter membrane support grooves 521 in the base 52 and the number of slots 542 in the silicone pad 54 are also set according to the number of filter membranes 53.

[0064] In one embodiment, such as Figure 5 As shown, the upper cover 51 also includes a frame 513, which extends downward from the bottom surface of the upper cover 51. Its shape corresponds to the filter membrane support groove 521, but its size is slightly larger than the filter membrane support groove 521. When the upper cover 51 is fitted onto the base 52, the bottom surface of the frame 513 abuts against the upper surface of the silicone pad 54, thereby pressing the silicone pad 54 down and adhering it to the base 52. Simultaneously, the inner surface of the frame 513 of the upper cover 51 adheres to the outer surface of the peripheral wall 543 of the silicone pad 54 (e.g., ...). Figure 6 (As shown), to achieve an airtight effect. Through the airtight filter chamber, the introduced airflow can be dispersed on the surface of the filter membrane 53.

[0065] In one embodiment, such as Figure 4 As shown, the base 52 also includes multiple ultrasonic welding ribs 525, which are multiple protrusions extending upward from the top surface of the base 52, and the silicone pad 54 includes openings 544 corresponding to the multiple ultrasonic welding ribs 525. When the upper cover 51 is fitted onto the base 52, the ultrasonic welding ribs 525 of the base 52 pass through the corresponding openings 544 on the silicone pad 54 and abut against the bottom surface of the upper cover 51, and the upper cover 51, silicone pad 54, filter membrane 53, and base 52 can be combined and sealed through the ultrasonic welding process.

[0066] In one embodiment, such as Figure 4 As shown, the filter membrane support groove 521 of the base 52 is provided with at least one second pneumatic opening 522A, and the filter membrane 53 has an extension 532. When the filter membrane 53 is housed in the filter membrane support groove 521, the extension 532 of the filter membrane 53 covers the second pneumatic opening 522A. In other words, the gas pipe flowing through the second pneumatic opening 522A (defined by the silicone pad 55, the top cover 51, the silicone pad 54, and the corresponding pneumatic opening on the base 52) will pass through the filter membrane 53 in the filter membrane support groove 521. When the gas pipe is opened and the external pneumatic driving device provides negative pressure through the gas pipe, the filtration mechanism of the aerosol filter 5 is activated.

[0067] like Figure 6As shown, when the external pneumatic drive device provides negative pressure through the gas pipe defined by the second pneumatic opening 522A (the direction of the driving pressure is shown by the thick black arrow), a negative pressure airflow (the direction of the airflow is shown by the thin black arrow) is generated to drive the fluid transport within the nucleic acid extraction cartridge C, for example, driving the reagents in the reaction tank into the waste liquid tank. When the negative pressure airflow flows through the filter membrane 53 of the aerosol filter 5, due to the hydrophobic properties of the filter membrane 53, water molecules in the airflow are blocked by the filter membrane 53, thereby filtering out salts dissolved in the aerosol and reducing the risk of salts flowing to upstream equipment (such as the external pneumatic drive device).

[0068] Figure 7 The analysis of the salt filtration efficiency of the aerosol filter in this case was performed using a chloride colorimetric assay kit to detect the salt content of the collected samples. The analyzed samples included deionized water control group samples (dH2O), samples without a filter membrane (no membrane), samples with a filter membrane and collected from waste liquid tank pathway 1 (with membrane -1), and samples with a filter membrane and collected from waste liquid tank pathway 2 (with membrane -2). Figure 7 The results show that the aerosol filter in this case can significantly reduce the salt content (by more than 80 times), or even eliminate the salt content altogether. Therefore, the aerosol filter proposed in this case for use in pressure-driven nucleic acid extraction cartridges can indeed effectively reduce the salt substances carried by aerosols during the nucleic acid extraction process.

[0069] In summary, this invention provides an aerosol filter for use in a pressure-driven nucleic acid extraction cartridge. By integrating a filter membrane structure within the cartridge, it filters aerosols carrying salt ions generated during nucleic acid extraction due to airflow, preventing blockages or corrosion / damage to upstream equipment pipes or electrical control components caused by salts. Based on the properties of the aerosol, the integrated filter membrane is hydrophobic with a pore size of 0.02–0.5 μm, effectively filtering water molecules in the airflow and thus salts. The filter membrane material can be selected based on the characteristics of the reagent or the size of the molecules to be filtered. Furthermore, since the aerosol filter is integrated into the nucleic acid extraction cartridge, it is disposable after testing, eliminating the need for regular maintenance and saving additional maintenance time.

[0070] Although the present invention has been described in detail by the above embodiments, and various modifications can be made by those skilled in the art, all of these modifications shall not depart from the scope of protection sought by the appended claims.

Claims

1. An aerosol filter, characterized in that, A nucleic acid extraction cartridge is applicable, comprising a liquid storage module, an extraction module, and a connecting module. The connecting module connects the liquid storage module and the extraction module and is connected to an external pneumatic drive device to drive fluid transport within the nucleic acid extraction cartridge. An aerosol filter is disposed between the connecting module and the external pneumatic drive device, and the aerosol filter includes: A top cover and a base, the top cover and the base defining a filter chamber; A filter membrane is stacked perpendicularly to the direction of gravity in a filter membrane support groove of the base; and A silicone pad is disposed between the top cover and the base, which is configured to make the filter cavity defined by the top cover and the base airtight; The top cover and the base have multiple corresponding filter membrane support structures, which respectively abut against the two opposite sides of the filter membrane to fix the filter membrane in the filter membrane bearing groove of the base, and together support the filter membrane to prevent it from deforming due to air pressure during the filtration process.

2. The aerosol filter as described in claim 1, characterized in that, The filter membrane is a polytetrafluoroethylene (PTFE) membrane.

3. The aerosol filter as described in claim 1, characterized in that, The pore size of the filter membrane is 0.02 ~ 0.5 μm.

4. The aerosol filter as described in claim 1, characterized in that, It also includes another silicone pad, which is disposed on the top surface of the cover and is structured on the gas pipe that connects to the external pneumatic drive device.

5. The aerosol filter as described in claim 1, characterized in that, The filter membrane carrier groove includes a filter membrane attachment area, which is a protrusion extending inward from the periphery of the filter membrane carrier groove.

6. The aerosol filter as described in claim 1, characterized in that, The multiple membrane support structures of the base are multiple protrusions extending upward from the bottom surface of the membrane carrier groove.

7. The aerosol filter as described in claim 1, characterized in that, The multiple filter membrane support structures of the top cover are multiple protrusions extending downward from the bottom surface of the top cover.

8. The aerosol filter as described in claim 1, characterized in that, The silicone pad includes a slot and a peripheral wall. The slot is provided corresponding to the filter membrane support groove, and the peripheral wall is provided at the periphery of the slot and protrudes from the upper and lower surfaces of the silicone pad.

9. The aerosol filter as described in claim 8, characterized in that, The top surface of the peripheral wall abuts against the bottom surface of the top cover, and the bottom surface of the peripheral wall abuts against the filter membrane.

10. The aerosol filter as described in claim 8, characterized in that, The top cover includes a frame that extends downward from the bottom surface of the top cover and abuts against the silicone pad, and the inner surface of the frame is in contact with the outer surface of the peripheral wall of the silicone pad.

11. The aerosol filter as claimed in claim 1, characterized in that, The base includes multiple ultrasonic welding ribs, which are multiple protrusions extending upward from the top surface of the base.

12. The aerosol filter as claimed in claim 1, characterized in that, The top cover, the base, and the silicone pad each have a number of corresponding pneumatic openings to collectively define multiple gas channels.

13. The aerosol filter as described in claim 12, characterized in that, At least one of the plurality of gas pipes passes through the filter membrane in the filter membrane carrier tank.

14. A nucleic acid extraction cartridge, characterized in that, include: A liquid storage module, an extraction module, and a communication module are provided. The communication module connects the liquid storage module and the extraction module and is connected to an external pneumatic drive device to drive the fluid transport within the nucleic acid extraction cartridge. as well as An aerosol filter is disposed between the connecting module and the external pneumatic drive device, and includes: A top cover and a base, the top cover and the base defining a filter chamber; A filter membrane is stacked perpendicularly to the direction of gravity in a filter membrane support groove of the base; and A silicone pad is disposed between the top cover and the base, which is configured to make the filter cavity defined by the top cover and the base airtight; The top cover and the base have multiple corresponding filter membrane support structures, which respectively abut against the two opposite sides of the filter membrane to fix the filter membrane in the filter membrane bearing groove of the base, and together support the filter membrane to prevent it from deforming due to air pressure during the filtration process.

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