Hybridoma cell strain secreting anti-fish cd28 monoclonal antibody and its application
By preparing and purifying the hybridoma cell line 1B6A2, which contains an anti-fish CD28 monoclonal antibody, the problem of the lack of tools for activating fish T cells was solved. This enabled the in vitro simulation of a second signal to activate T cells and promote their proliferation, providing an effective tool for adaptive immune responses and disease control in fish.
Patent Information
- Application Number
- CN202310436759.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Priority Date
- 2022-05-06
- Filing Date
- 2023-04-21
- Publication Date
- 2026-02-10
- Estimated Expiration
- 2043-04-21
AI Technical Summary
Currently, there is a lack of effective activation tools in fish T cell research, especially for simulating T cell activation and proliferation in vitro. Furthermore, the regulatory mechanism of CD28 molecules in Nile tilapia is unclear, which affects research on its adaptive immune response.
A hybridoma cell line 1B6A2 that secretes a monoclonal antibody against fish CD28 was provided. The monoclonal antibody against the fish T lymphocyte surface membrane protein CD28 was prepared and purified by lentivirus-mediated expression of the fish CD28 gene and used to mimic the second signal to activate fish T cells.
A monoclonal antibody that specifically recognizes and binds to the CD28 protein in fish was successfully prepared. It can mimic the second signal to activate T cells in vitro and promote their proliferation, providing a tool for studying adaptive immune responses in fish and can be applied to the development of fish disease prevention and control reagents and vaccines.