A method for analyzing and determining soil heavy metals based on enzyme inhibition method

Abstract

The application discloses a soil heavy metal analysis and determination method based on an enzyme inhibition method, and comprises the following specific steps: S1, taking soil: a certain amount of soil to be detected is taken for standby; S2, placing into a mixing tube: the soil is quantitatively placed into a mixing tube; S3, preparing a detection solution: the soil is mixed with water, a buffer solution and an enzyme in the mixing tube to prepare a detection solution for standby; S4, color development: the detection solution is directly subjected to preliminary color development in the mixing tube; and S5, colorimetric detection by a detector: the buffer solution in the mixing tube is subjected to fine colorimetric detection by using the detector; the mixing tube placed into the mixing tube in the step S2 comprises a soil taking tube, a top cover is covered on the soil taking tube, and a shaking mechanism is connected to the top end of the top cover, the soil heavy metal analysis and determination method based on the enzyme inhibition method can directly avoid the step of outdoor weighing, is simple to operate, and can guarantee the accuracy of detection results.

Description

Technical Field

[0001] This invention relates to the field of soil heavy metal detection technology, and in particular to a method for soil heavy metal analysis and determination based on enzyme inhibition. Background Technology

[0002] Bioassay refers to the technology of evaluating soil pollution from a biological perspective by detecting the response of individual organisms, populations or communities growing or living in the soil to heavy metals. It mainly includes enzyme inhibition method and biosensor method.

[0003] When using the enzyme inhibition method to analyze and determine the heavy metal content in soil, soil samples are usually brought into the laboratory, where the test solution is mixed and then colorimetrically determined using a detector to obtain accurate analytical results. However, because the detector is not portable, it is not possible to conduct tests on-site, which is not conducive to the rapid formation of soil heavy metal analysis results. Although there are already test strips on the market that rapidly test heavy metal content based on the enzyme inhibition method, the test solution is prepared outdoors, and its preparation ratio and whether other substances have entered the test solution cannot be guaranteed, which directly leads to inaccurate test results. Summary of the Invention

[0004] This invention discloses a method for analyzing and determining heavy metals in soil based on enzyme inhibition, aiming to solve the technical problem that the preparation ratio and whether other substances enter the test solution cannot be guaranteed when the test solution is prepared outdoors, which directly leads to inaccurate test results.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A method for determining heavy metals in soil based on enzyme inhibition includes the following specific steps:

[0007] S1: Soil sampling: Take a certain amount of soil that needs to be tested for later use;

[0008] S2: Place in mixing tube: Quantitatively place it into the mixing tube;

[0009] S3: Preparation of test solution: Mix soil with water, buffer solution and enzyme in a mixing tube to prepare test solution for later use;

[0010] S4: Color development: Perform preliminary color development and judgment on the test solution directly in the mixing tube;

[0011] S5: Colorimetric analysis using a detector: Use a detector to perform a fine colorimetric analysis of the buffer solution in the mixing tube;

[0012] In step S2, the mixing tube placed in the mixing tube includes a soil sampling tube, which is covered with a top cover. A vibration mechanism is snapped onto the top of the top cover. A buffer mixing tube is threaded to the bottom of the soil sampling tube, and a colorimetric tube is threaded to the bottom of the buffer mixing tube. A sealing mechanism is provided at the connection between the colorimetric tube and the buffer mixing tube, and a drawer is provided at the connection between the soil sampling tube and the buffer mixing tube. A colorimetric test strip is provided on one inner wall of the colorimetric tube.

[0013] By incorporating a mixing tube, soil samples are taken outdoors. The amount of soil to be collected is determined by placing the soil sample in the tube, which is marked with its capacity. A measured amount of water is added, and the tube is then sealed. The extraction plate is removed, allowing the soil, water, and the quantitative buffer solution in the mixing tube to mix rapidly. The sealing mechanism is then opened, allowing the mixture to mix quickly with the enzyme in the colorimetric tube. The mixture is then developed using colorimetric strips. Throughout this process, the system remains relatively sealed, effectively preventing the entry of other substances. Since the final mixed test solution is placed in the colorimetric tube, it can be directly inserted into the detector for further testing. Furthermore, because both the enzyme and buffer solution are quantitatively set, the outdoor weighing step is eliminated, simplifying the operation and ensuring accurate test results.

[0014] In a preferred embodiment, the sealing mechanism includes a movable sealing ring. A connecting rod is fixed to the inner wall of the movable sealing ring, and a lever is fixed to the outer wall of the other side of the movable sealing ring. A groove is provided at the connection between the lever and the movable sealing ring. A semi-circular hollow shell is fixedly connected to one inner wall of the colorimetric tube, and a semi-circular sealing plate is movably connected inside the semi-circular hollow shell. The semi-circular hollow shell and the semi-circular sealing plate are concentric. The outer wall of the semi-circular sealing plate movably fits against the inner wall of the colorimetric tube, and the connecting rod is fixed to the top of the semi-circular sealing plate. A guide plate is fixed to the inner wall of the buffer mixing tube, and the bottom end of the guide plate corresponds to the opening of the semi-circular hollow shell. A cellulose membrane is provided on the surface of the guide plate, and the buffer solution is attached to the cellulose membrane. A second cellulose membrane is provided at the bottom of the colorimetric tube, and the enzyme is attached to the second cellulose membrane.

[0015] With a sealing mechanism, when the mixture and enzyme are mixed, the movable sealing ring is rotated by turning the lever, opening the opposite side of the semi-circular hollow shell. Under the action of the guide plate, the mixture falls and mixes with the enzyme. After falling, turning the lever in the opposite direction returns the colorimetric tube to the sealed state. In the sealed state, it is easy to shake and mix, while further ensuring that the mixing tube will not leak liquid or allow other substances to enter during movement. At the same time, the cellulose membrane and the second cellulose membrane are set up so that the buffer and enzyme adhere to them, thus ensuring the stability of their movement in the empty tube state and preventing the enzyme from prematurely contacting the colorimetric strip and affecting the colorimetric results.

[0016] In a preferred embodiment, the oscillation mechanism includes a snap-fit ​​frame, which is movably snapped onto both sides of the top cover. A support plate is fixed to the top of the snap-fit ​​frame, and a limit tube is provided at the top of the support plate. A support rod is movably connected inside the limit tube, and the support rod passes through the support plate. A pull plate is fixed to the top of the support rod, and an impact plate is fixed to the bottom of the support rod.

[0017] S3, the preparation of the detection solution includes the following specific steps:

[0018] S31: Add water: Add appropriate amount of water into the soil sampling pipe to mix it thoroughly with the soil;

[0019] S32: Mixing buffer: Open the slide plate to allow the watered soil to enter the buffer mixing tube and mix with the buffer;

[0020] S33: Vibration: The vibration mechanism is used to impact the top cover, causing the soil to fall fully into the buffer solution mixing tube;

[0021] S34: Mixed enzyme: Open the sealing mechanism to allow the mixture to fall into the colorimetric tube;

[0022] S35: Shaking: Close the sealing mechanism and shake the colorimetric tube to fully mix the test solution.

[0023] By incorporating a vibration mechanism, the soil inside the sampling tube may adhere to the inner wall of the tube due to its stickiness during the fall. At this point, the vibration mechanism is engaged with the top cover, and the support rod is pulled to compress the spring. Then, the spring is released, causing the impact plate to strike the top cover quickly, causing the soil inside the sampling tube to fall into the buffer solution mixing tube. This ensures the accuracy of soil testing, and the vibration mechanism is reusable.

[0024] As shown above, a method for analyzing and determining heavy metals in soil based on enzyme inhibition includes the following specific steps:

[0025] S1: Soil sampling: Take a certain amount of soil that needs to be tested for later use;

[0026] S2: Place in mixing tube: Quantitatively place it into the mixing tube;

[0027] S3: Preparation of test solution: Mix soil with water, buffer solution and enzyme in a mixing tube to prepare test solution for later use;

[0028] S4: Color development: Perform preliminary color development and judgment on the test solution directly in the mixing tube;

[0029] S5: Colorimetric analysis using a detector: Use a detector to perform a fine colorimetric analysis of the buffer solution in the mixing tube;

[0030] In step S2, the mixing tube placed in the mixing tube includes a soil sampling tube covered with a top cap, and a vibration mechanism is snapped onto the top of the top cap. A buffer solution mixing tube is threadedly connected to the bottom of the soil sampling tube, and a colorimetric tube is threadedly connected to the bottom of the buffer solution mixing tube. A sealing mechanism is provided at the connection between the colorimetric tube and the buffer solution mixing tube, and a drawer is provided at the connection between the soil sampling tube and the buffer solution mixing tube. A colorimetric test strip is provided on one inner wall of the colorimetric tube. The soil heavy metal analysis and determination method based on enzyme inhibition provided by this invention has the technical advantage of directly avoiding outdoor weighing steps, simplifying operation, and ensuring the accuracy of test results. Attached Figure Description

[0031] Figure 1 This is a schematic diagram of the overall structure of the mixing tube for a soil heavy metal analysis and determination method based on enzyme inhibition proposed in this invention.

[0032] Figure 2 This is a schematic diagram of the oscillation mechanism of the mixing tube in a soil heavy metal analysis and determination method based on enzyme inhibition proposed in this invention.

[0033] Figure 3 This is a cross-sectional view of the mixed tube in a soil heavy metal analysis and determination method based on enzyme inhibition proposed in this invention.

[0034] Figure 4 This is a schematic diagram of the closed mechanism of the mixing tube in a soil heavy metal analysis and determination method based on enzyme inhibition proposed in this invention.

[0035] Figure 5 This is an overall flowchart of a soil heavy metal analysis and determination method based on enzyme inhibition proposed in this invention.

[0036] Figure 6 This is a flowchart illustrating the preparation of the detection solution for a soil heavy metal analysis and determination method based on enzyme inhibition proposed in this invention.

[0037] In the diagram: 1. Vibration mechanism; 2. Top cover; 3. Soil sampling tube; 4. Pulling plate; 5. Buffer mixing tube; 6. Colorimetric tube; 7. Sealing mechanism; 8. Colorimetric test paper; 9. Guide plate; 10. Cellulose membrane; 11. Second cellulose membrane; 101. Pulling plate; 102. Limiting tube; 103. Support plate; 104. Snap-fit ​​frame; 105. Impact plate; 106. Spring; 107. Support rod; 701. Pulley; 702. Moving sealing ring; 703. Connecting rod; 704. Semi-circular hollow shell; 705. Semi-circular sealing plate. Detailed Implementation

[0038] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0039] The method for determining soil heavy metals based on enzyme inhibition disclosed in this invention is mainly applied to the analysis of soil heavy metals.

[0040] Reference Figure 1 , Figure 3 and Figure 5 A method for determining heavy metals in soil based on enzyme inhibition includes the following specific steps:

[0041] S1: Soil sampling: Take a certain amount of soil that needs to be tested for later use;

[0042] S2: Place in mixing tube: Quantitatively place it into the mixing tube;

[0043] S3: Preparation of test solution: Mix soil with water, buffer solution and enzyme in a mixing tube to prepare test solution for later use;

[0044] S4: Color development: Perform preliminary color development and judgment on the test solution directly in the mixing tube;

[0045] S5: Colorimetric analysis using a detector: Use a detector to perform a fine colorimetric analysis of the buffer solution in the mixing tube;

[0046] S2, the mixing tube placed in the mixing tube includes a soil sampling tube 3, and the soil sampling tube 3 is covered with a top cover 2. A vibration mechanism 1 is snapped onto the top of the top cover 2. A buffer mixing tube 5 is threadedly connected to the bottom of the soil sampling tube 3, and a colorimetric tube 6 is threadedly connected to the bottom of the buffer mixing tube 5. A sealing mechanism 7 is provided at the connection between the colorimetric tube 6 and the buffer mixing tube 5, and a drawer 4 is provided at the connection between the soil sampling tube 3 and the buffer mixing tube 5. A colorimetric test strip 8 is provided on one inner wall of the colorimetric tube 6. When sampling outdoors, soil is placed in the soil sampling tube 3. The amount of soil taken can be determined according to the capacity and markings of the soil sampling tube 3. After adding a quantitative amount of water and covering with the top cover 2... The entire mixing tube is sealed. Then, by removing the extraction plate 4, the quantitative buffer solution in the soil, water and buffer solution mixing tube 5 is quickly mixed. Then, by opening the sealing mechanism 7, it is quickly mixed with the enzyme in the colorimetric tube 6. The mixing result is then developed by the colorimetric test strip 8. Throughout the process, it is in a relatively sealed state, which effectively prevents the entry of other substances. Since the final mixed test solution is placed in the colorimetric tube 6, the colorimetric tube 6 can be directly placed into the detector for further detection. At the same time, since the enzyme and buffer solution are quantitatively set, the outdoor weighing step can be directly avoided. The operation is simple and the accuracy of the test results can be guaranteed.

[0047] Reference Figure 4 In a preferred embodiment, the sealing mechanism 7 includes a movable sealing ring 702. A connecting rod 703 is fixed to the inner wall of the movable sealing ring 702, and a lever 701 is fixed to the outer wall of the other side of the movable sealing ring 702. A groove is provided at the connection between the lever 701 and the movable sealing ring 702.

[0048] Reference Figure 4 In a preferred embodiment, a semi-circular hollow shell 704 is fixedly connected to one inner wall of the colorimetric tube 6, and a semi-circular sealing plate 705 is movably connected inside the semi-circular hollow shell 704, while the semi-circular hollow shell 704 and the semi-circular sealing plate 705 are concentric.

[0049] Reference Figure 3 and Figure 4 In a preferred embodiment, the outer wall of the semicircular sealing plate 705 is movably attached to the inner wall of the colorimetric tube 6, and the connecting rod 703 is fixed to the top of the semicircular sealing plate 705. The inner wall of the buffer mixing tube 5 is fixed with a guide plate 9, and the bottom end of the guide plate 9 corresponds to the opening of the semicircular hollow shell 704.

[0050] Reference Figure 3 In a preferred embodiment, a cellulose membrane 10 is provided on the surface of the guide plate 9, and the buffer solution is attached to the cellulose membrane 10. A second cellulose membrane 11 is provided at the bottom of the colorimetric tube 6, and the enzyme is attached to the second cellulose membrane 11. When the mixture and enzyme are mixed, the movable sealing ring 702 is rotated by turning the lever 701, thereby causing the semi-circular sealing plate 705 to be hidden inside the semi-circular hollow shell 704, opening the opposite side of the semi-circular hollow shell 704. Under the action of the guide plate 9, the mixture falls and mixes with the enzyme. After falling, the lever 701 is turned in the opposite direction, and the colorimetric tube 6 can return to the sealed state. In the sealed state, it is convenient to shake and mix, and at the same time, it further ensures that the mixing tube will not cause liquid leakage or the entry of other substances during the movement. At the same time, the cellulose membrane 10 and the second cellulose membrane 11 are provided so that the buffer solution and enzyme adhere to them, thereby ensuring the stability of the movement of the two in the empty tube state, and also avoiding the enzyme from contacting the colorimetric test paper 8 in advance and affecting the colorimetric results.

[0051] Reference Figure 1 and Figure 2 In a preferred embodiment, the oscillation mechanism 1 includes a snap-fit ​​bracket 104, which is movably snapped onto both sides of the top cover 2. A support plate 103 is fixed to the top of the snap-fit ​​bracket 104, and a limit tube 102 is provided at the top of the support plate 103.

[0052] Reference Figure 2In a preferred embodiment, a support rod 107 is movably connected inside the limiting tube 102, and the support rod 107 passes through the support plate 103. A pull plate 101 is fixed to the top end of the support rod 107, and an impact piece 105 is fixed to the bottom end of the support rod 107.

[0053] Reference Figure 2 In a preferred embodiment, the top end of the impact piece 105 is connected to a spring 106, and the other end of the spring 106 is fixed to the inner wall of the top end of the limiting tube 102, while the bottom end of the impact piece 105 is attached to the top end of the top cover 2.

[0054] Reference Figure 6 In a preferred embodiment, step S3, the preparation of the detection solution includes the following specific steps:

[0055] S31: Add water: Add appropriate amount of water into soil sampling pipe 3 to mix it thoroughly with the soil;

[0056] S32: Mixing buffer: Open the drawer 4 to allow the water-added soil to enter the buffer mixing tube 5 and mix with the buffer;

[0057] S33: Oscillation: Use the oscillation mechanism 1 to strike the top cover 2, so that the soil can fall fully into the buffer solution mixing tube 5;

[0058] S34: Mixed enzyme: Open the sealing mechanism 7 to allow the mixed solution to fall into the colorimetric tube 6;

[0059] S35: Shaking: Close the sealing mechanism 7, shake the colorimetric tube 6 to fully mix the test solution. During the falling process, the soil in the soil sampling tube 3 may adhere to the inner wall of the soil sampling tube 3 due to its stickiness. At this time, the vibration mechanism 1 is snapped onto the top cover 2, and the support rod 107 is pulled to compress the spring 106. Then the spring 106 is released, so that the impact piece 105 quickly impacts the top cover 2, causing the soil inside the soil sampling tube 3 to fall into the buffer mixing tube 5. This ensures the accuracy of the soil test and the vibration mechanism 1 can be reused.

[0060] Working Principle: When sampling outdoors, soil is placed in the soil sampling tube 3. The amount of soil to be sampled is determined based on the capacity and markings of the soil sampling tube 3. After adding a quantitative amount of water and covering it with the top cover 2, the entire mixing tube is sealed. Then, by removing the extraction plate 4, the soil, water, and the quantitative buffer solution in the buffer mixing tube 5 are quickly mixed. Then, by opening the sealing mechanism 7, it is quickly mixed with the enzyme in the colorimetric tube 6. The mixing result is then developed by the colorimetric test strip 8. Throughout the process, it is in a relatively sealed state, which effectively prevents the entry of other substances. Since the final mixed test solution is placed in the colorimetric tube 6, the colorimetric tube 6 can be directly placed into the detector for further detection. At the same time, since both the enzyme and buffer solution are quantitatively set, the outdoor weighing step can be directly avoided. The operation is simple and the accuracy of the test results can be guaranteed. When the mixed solution and enzyme are mixed, by moving the lever 701, the moving sealing ring 702 is rotated, which causes the semi-circular sealing plate 705 to be hidden in the semi-circular hollow shell 704, making the semi-circular hollow shell 704... The opposite side of the shell 704 opens, allowing the mixture to fall and mix with the enzyme under the action of the guide plate 9. After falling, the lever 701 is reversed to return to the sealed state of the colorimetric tube 6. In the sealed state, it is easy to shake and mix, and at the same time, it further ensures that the mixing tube will not leak liquid or allow other substances to enter during the movement. At the same time, the cellulose membrane 10 and the second cellulose membrane 11 are set so that the buffer and enzyme adhere to them, thereby ensuring the stability of their movement in the empty tube state and preventing the enzyme from contacting the colorimetric test paper 8 in advance and affecting the colorimetric results. In addition, the soil in the soil sampling tube 3 may adhere to the inner wall of the soil sampling tube 3 due to the stickiness of the soil during the fall. At this time, the shaking mechanism 1 is snapped onto the top cover 2, and the support rod 107 is pulled to compress the spring 106. Then the spring 106 is released, so that the impact piece 105 quickly impacts the top cover 2, causing the soil inside the soil sampling tube 3 to fall into the buffer mixing tube 5. This ensures the accuracy of soil detection, and the shaking mechanism 1 can be reused.

[0061] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A method for analyzing and determining heavy metals in soil based on enzyme inhibition, characterized in that, The specific steps include the following: S1: Soil sampling: Take a certain amount of soil that needs to be tested for later use; S2: Place in mixing tube: Quantitatively place it into the mixing tube; S3: Preparation of test solution: Mix soil with water, buffer solution and enzyme in a mixing tube to prepare test solution for later use; S4: Color development: Perform preliminary color development and judgment on the test solution directly in the mixing tube; S5: Colorimetric analysis using a detector: Use a detector to perform a fine colorimetric analysis of the buffer solution in the mixing tube; The S2, the mixing tube placed in the mixing tube includes a soil sampling tube (3), and the soil sampling tube (3) is covered with a top cover (2), and the top of the top cover (2) is snapped with a vibration mechanism (1). The bottom end of the soil sampling tube (3) is connected to a buffer mixing tube (5) by a thread, and the bottom end of the buffer mixing tube (5) is connected to a colorimetric tube (6) by a thread. A sealing mechanism (7) is provided at the connection between the colorimetric tube (6) and the buffer mixing tube (5), and a draw plate (4) is provided at the connection between the soil sampling tube (3) and the buffer mixing tube (5). A colorimetric test paper (8) is provided on one side of the inner wall of the colorimetric tube (6). The closing mechanism (7) includes a movable sealing ring (702), and a connecting rod (703) is fixed on the inner wall of the movable sealing ring (702). A lever (701) is fixed on the outer wall of the other side of the movable sealing ring (702), and a groove is provided at the connection between the lever (701) and the movable sealing ring (702). A semi-circular hollow shell (704) is fixedly connected to one side of the inner wall of the colorimetric tube (6), and a semi-circular sealing plate (705) is movably connected inside the semi-circular hollow shell (704). At the same time, the semi-circular hollow shell (704) and the semi-circular sealing plate (705) are concentric. The outer wall of the semicircular sealing plate (705) is movably attached to the inner wall of the colorimetric tube (6), and the connecting rod (703) is fixed at the top of the semicircular sealing plate (705). The inner wall of the buffer mixing tube (5) is fixed with a guide plate (9), and the bottom end of the guide plate (9) corresponds to the opening of the semicircular hollow shell (704). The surface of the guide plate (9) is provided with a cellulose membrane (10), and the buffer solution is attached to the cellulose membrane (10). The bottom end of the colorimetric tube (6) is provided with a second cellulose membrane (11), and the enzyme is attached to the second cellulose membrane (11).

2. The method for determining soil heavy metals based on enzyme inhibition according to claim 1, characterized in that, The oscillation mechanism (1) includes a snap-fit ​​bracket (104), which is movably snapped onto both sides of the top cover (2). A support plate (103) is fixed to the top of the snap-fit ​​bracket (104), and a limit tube (102) is provided at the top of the support plate (103).

3. The method for determining soil heavy metals based on enzyme inhibition according to claim 2, characterized in that, A support rod (107) is movably connected inside the limiting tube (102), and the support rod (107) passes through the support plate (103). A pull plate (101) is fixed at the top of the support rod (107), and an impact piece (105) is fixed at the bottom of the support rod (107).

4. The method for determining soil heavy metals based on enzyme inhibition according to claim 3, characterized in that, The top end of the impact piece (105) is connected to a spring (106), and the other end of the spring (106) is fixed to the inner wall of the top end of the limiting tube (102). The bottom end of the impact piece (105) is attached to the top end of the top cover (2).

5. The method for determining soil heavy metals based on enzyme inhibition according to claim 4, characterized in that, S3, the preparation of the detection solution includes the following specific steps: S31: Add water: Add appropriate amount of water into the soil sampling pipe (3) to mix it thoroughly with the soil; S32: Mixing buffer: Open the drawer (4) to allow the watered soil to enter the buffer mixing tube (5) and mix with the buffer; S33: Oscillation: Use the oscillation mechanism (1) to strike the top cover (2) to make the soil fall fully into the buffer mixing tube (5); S34: Mixed enzyme: Open the sealing mechanism (7) to allow the mixture to fall into the colorimetric tube (6); S35: Shaking: Close the sealing mechanism (7), shake the colorimetric tube (6) to fully mix the test solution.

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