A molecular marker, primer, kit and application for identifying non-ribosomal polypeptide synthetase dtxS1 of fruit anthracnose fungus

By comparing the whole genome sequence of anthrax related to anthrax of oleifera, the specific coding sequence of anthrax of oleifera was screened out and specific primers were designed, which solved the problem of identification and detection of anthrax of oleifera in the prior art, and achieved high specificity and high sensitivity detection effects.

CN117051144BActive Publication Date: 2025-03-28CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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Patent Information

Application Number
CN202211552871.5
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-12-06
Publication Date
2025-03-28
Estimated Expiration
2042-12-06

AI Technical Summary

Technical Problem

The prior art lacks simple and effective methods to identify and detect raw anthrax bacteria of oleifera fruit, which makes it difficult to diagnose and control anthrax of oleifera.

Method used

By comparing the whole genome sequence of anthrax related to anthrax of oleifera, the specific coding sequence of anthrax of fruit was screened out, and the non-ribosomal polypeptide synthetase dtxS1 was selected as a molecular marker, and specific primers were designed for PCR detection.

Benefits of technology

The identification and detection of high specificity and high sensitivity of the fruit anthrax bacteria is achieved, which can distinguish different anthrax bacteria species and other plant disease-related fungi, and provides an accurate diagnosis strategy for oleifera anthrax.

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Abstract

The present invention discloses a non-ribosomal polypeptide synthetase dtxS1 molecular marker, primer, kit and application for identifying fruit anthracnose. The whole genome sequence information of several Colletotrichum fungi closely related to oil-tea anthracnose is used to perform comparative genome analysis, obtain the specific chromosome sequence and candidate gene of fruit anthracnose, and further select the candidate gene that can be used for specific identification of fruit anthracnose according to the possible gene function of the candidate gene and whether it contains introns and other characteristics as a molecular marker, and use the marker to specifically detect oil-tea fruit anthracnose. The present invention can be used for the diagnosis and prediction of oil-tea anthracnose, and can also be used for the specific detection of fruit anthracnose.
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