A method for removing extracellular DNA / extracellular antibiotic resistance genes in red soil

By degrading extracellular DNA and resistance genes in red soil with eDNA removers, the problem of extracellular DNA/extracellular antibiotic resistance gene contamination in red soil was solved, and efficient removal and reduction of the risk of environmental resistance transmission were achieved.

CN117102227BActive Publication Date: 2025-10-17NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202310935735.2
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-07-28
Publication Date
2025-10-17
Estimated Expiration
2043-07-28

AI Technical Summary

Technical Problem

The contamination of extracellular DNA/extracellular antibiotic resistance genes in red soil leads to the risk of environmental resistance transmission, which is difficult to effectively remove with existing technologies.

Method used

The extracellular DNA and resistance genes in red soil were degraded by using eDNA removal agents including citric acid-sodium citrate buffer, L-ascorbic acid, copper sulfate pentahydrate, ethylenediaminetetraacetic acid and hydrogen peroxide through standing and centrifugation steps.

Benefits of technology

The removal rate of extracellular DNA in red soil exceeds 95%, the intracellular DNA is not affected, and the abundance of extracellular antibiotic resistance genes is significantly reduced, reducing the risk of environmental resistance transmission.

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Abstract

The application discloses a method for removing extracellular DNA / antibiotic resistance genes in red soil, and belongs to the field of environmental pollution treatment. The application mainly solves the problem of extracellular DNA / antibiotic resistance gene pollution in red soil. The application is based on an extracellular DNA (eDNA) remover, a chemical reagent, which is used for degrading the extracellular DNA in the red soil, and then removing antibiotic resistance genes (eARGs) contained in the extracellular DNA, so as to help people correctly evaluate the risk of resistance gene transmission.
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