A pesticide composition for controlling planococcus krauniae

Abstract

The present application belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating Dialeuroides scutellatus. The pesticide composition for preventing and treating Dialeuroides scutellatus comprises, as effective components, methopipentril ethyl ester, emamectin benzoate, cycloprothrin or fluazinam, which are compounded in a mass ratio of 1-400:400-1. The methopipentril ethyl ester is compounded and screened with other insecticidal active ingredients, and compared with a single effective component, the prevention and treatment effect on Dialeuroides scutellatus can be improved, which provides a reference for scientifically and rationally using the pesticide to prevent and treat the newly-invasive pest Dialeuroides scutellatus and formulating scientific and effective prevention and control measures. The pesticide composition for preventing and treating Dialeuroides scutellatus can delay the generation and development of the drug resistance of the prevention and treatment object Dialeuroides scutellatus, relieve environmental pressure, reduce pesticide residues and is green and environment-friendly.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, and specifically discloses a pesticide composition for controlling papaya mealybug. Background Technology

[0002] The papaya mealybug (Paracoccus marginalus Williams and Granara de Willink), also known as the papaya powdery scale, is an important global quarantine pest. It has a wide range of applications, with recorded damage to over 260 species from 64 families. Papaya, mango, custard apple, banana, guava, and tuber crops are its primary hosts. The papaya mealybug primarily feeds as nymphs and adult females by piercing and sucking sap from the stems, leaves, flowers, and fruits of its host plants, causing leaf curling, deformities, and fruit drop. It also easily induces sooty mold, severely impacting fruit yield and quality.

[0003] The papaya mealybug has a high reproductive capacity, multiplying rapidly under suitable conditions. To prevent its spread, rapid and effective control measures are needed once damage is detected. Chemical control is a quick method for controlling the papaya mealybug in a short period. However, single chemical agents have limited control spectrum, and long-term use can easily lead to resistance in the target organisms, resulting in reduced or even ineffective efficacy, and exacerbating environmental pollution and pesticide residues.

[0004] Spiropidion (CAS: 1229023-00-0) is a spirocyclic quaternary ketoacid insecticide developed by Syngenta. The International Pesticide Resistance Action Committee classifies spiropidion as Class 23: Acetyl-CoA carboxylase (ACCase) inhibitor, quaternary ketoacid, and quaternary ketoacid derivatives. Its mechanism of action is as a lipid biosynthesis inhibitor, disrupting lipid synthesis by inhibiting the activity of ACCase during fat synthesis in pests, thereby blocking normal energy metabolism and ultimately leading to pest death. Spiropidion can be used to control pests and mites such as aphids, whiteflies, psyllids, scale insects, soft scale insects, spider mites, spider mites, and cotton aphids.

[0005] By combining and screening chemical agents with different components, the shortcomings of single agents can be effectively overcome. Currently, there are no reports of combining methoxypiperidine ethyl ester with emamectin benzoate, cyprodinil, or fluazinam.

[0006] The information disclosed in this background section is intended only to enhance the understanding of the overall background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Summary of the Invention

[0007] The purpose of this invention is to provide a pesticide composition for controlling papaya mealybug, which can improve the control effect against papaya mealybug compared with a single active ingredient, and can also solve the defects of single agents.

[0008] To achieve the above objectives, the present invention provides the following technical solution:

[0009] A pesticide composition for controlling papaya mealybug, the active ingredient of which is compounded with methoxypiperidine ethyl ester, abamectin, cyprodinil or fluazinam in a mass ratio of 1-400:400-1.

[0010] Preferably, the mass ratio of methoxypiperidine ethyl ester to emamectin benzoate is 1-50:20-1.

[0011] Preferably, the mass ratio of methoxypiperidine ethyl ester to cyclopropionate is 1-8:15-1.

[0012] Preferably, the mass ratio of methoxypiperidine ethyl ester to fluazinam is 1-20:10-1.

[0013] The present invention also provides an insecticide for controlling papaya mealybug, comprising the aforementioned pesticide composition for controlling papaya mealybug and pesticide auxiliary ingredients.

[0014] Preferably, the pesticide auxiliary ingredients are selected from one or more of the following: wetting agents, dispersants, emulsifiers, solvents, cosolvents, inert fillers, disintegrants, thickeners, film-forming agents, defoamers, antifreeze agents, preservatives, and colorants.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] (1) This invention combines and screens methoxypiperidine ethyl ester with other insecticidal active ingredients. Compared with single active ingredients, it can improve the control effect on papaya mealybug, and provide a reference for the scientific and rational use of pesticides to control the newly introduced pest papaya mealybug and to formulate scientific and effective control measures.

[0017] (2) The pesticide composition of the present invention for controlling papaya mealybug can delay the emergence and development of pesticide resistance in the target papaya mealybug, alleviate environmental pressure and reduce pesticide residues, and is green and environmentally friendly. Detailed Implementation

[0018] The technical solution of this invention patent will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention.

[0019] Example Indoor bioactivity test

[0020] 1. Test subjects

[0021] Papaya mealybugs were collected from papaya plants and continuously reared for three generations in an artificial climate chamber with a temperature of 26±1℃, relative humidity of 75±5%, and 14 hours of light and 10 hours of darkness. Second-instar nymphs of similar size and good health were selected as the test subjects.

[0022] 2. Test reagents

[0023] 98% methoxypiperazine technical grade, 95% abamectin, 98.6% cyprodinil technical grade, 99% fluazinam technical grade.

[0024] The original drug was first dissolved in dimethyl sulfoxide and then diluted with 0.1% Tween-80 aqueous solution to prepare a single-dose stock solution. Multiple formulations were prepared, and each single-dose and each formulation mixture was prepared with 5 gradient mass concentrations according to the proportional method. Each mass concentration solution was 50 mL and ready for use.

[0025] 3. Test Methods

[0026] The leaf immersion method was used. Potato leaves infested with second-instar nymphs of the papaya mealybug were immersed in the pesticide solution for 15 seconds. They were then removed, placed on filter paper to wash away excess pesticide, and allowed to air dry. The leaves were then placed in 9cm diameter agar-coated culture dishes and kept in an artificial climate chamber at 26±1℃ and 75±5% relative humidity, with 14 hours of light and 10 hours of darkness for further rearing. One leaf was placed in each culture dish, with approximately 30 second-instar nymphs per leaf. Three leaves were treated with each concentration of pesticide solution, and a 0.1% Tween-80 solution was used as a blank control. After 36 hours, the total number of insects and the number of dead insects were recorded for each treatment, and the corrected mortality rate for each treatment was calculated.

[0027]

[0028] In the above formula: P -- mortality rate, in %; K -- number of dead insects; N -- total number of insects treated.

[0029]

[0030] In the above formula: P1 -- corrected mortality rate, in %; P t --Treatment mortality rate, in %; P0--Control mortality rate, in %.

[0031] 4. Data Analysis

[0032] Regression analysis was performed using DPS software on the logarithmic values ​​of drug concentrations and the corrected mortality rates for each treatment to calculate the LC ratio of each drug. 50The co-toxicity coefficient (CTC value) of the mixture was calculated according to Sun Yunpei's method, and the synergistic effect of the drug was evaluated based on the calculated CTC value. The results are shown in Table 1-4.

[0033] Table 1. Results of in vitro bioactivity assays of the combination of methoxypiperidine and emamectin benzoate on Papaver somniferum mealybug.

[0034] Drug Name and Proportion <![CDATA[LD 50 (mg / L)]]> ATI TTI CTC Ethyl methoxypiperidine 3.7941 100.0000 -- -- abamectin 10.3682 36.5936 -- -- Ethyl methoxypiperidine 1: 20g emamectin benzoate 6.9363 54.6992 39.6130 138.0840 Ethyl methoxypiperidine 1: 10 emamectin benzoate 5.5302 68.6069 42.3578 161.9698 Ethyl methoxypiperidine 1: 5 emamectin benzoate 6.2273 60.9269 47.1614 129.1882 Ethyl methoxypiperidine 5: 1 emamectin benzoate 4.5291 83.7716 68.2968 122.6582 Ethyl methoxypiperidine 10: 1 emamectin benzoate 3.0635 123.8485 89.4323 138.4831 Ethyl methoxypiperidine 20: 1 emamectin benzoate 1.5009 252.7883 94.2358 268.2509 Ethyl methoxypiperidine 40: 1 emamectin benzoate 2.5629 148.0393 96.9806 152.6483 Ethyl methoxypiperidine 50: 1 emamectin benzoate 2.1343 177.7679 98.4535 180.5602

[0035] As shown in Table 1, when methoxypiperidine ethyl ester and emamectin benzoate are combined in a mass ratio of 1-50:20-1, the co-toxicity coefficient against papaya mealybug is greater than 120, indicating a synergistic effect.

[0036] Table 2. Results of indoor bioactivity assays of the combined methoxypiperidine and cyprofen on the papaya mealybug.

[0037] Drug Name and Proportion <![CDATA[LD 50 (mg / L)]]> ATI TTI CTC Ethyl methoxypiperidine 3.7941 100.0000 -- -- Cyprofen 2.5198 150.5715 -- -- Methoxypiperidine ethyl ester 1: Cyprodinil 15 2.1429 177.0545 147.4108 120.1096 Methoxypiperidine ethyl ester 1: Cyprofen 7 1.8060 210.0831 144.2500 145.6381 Methoxypiperidine ethyl ester 1: Cyprofen 3 0.5369 706.6679 137.9286 512.3433 Methoxypiperidine ethyl ester 1: Cyprofen 1 2.4200 156.7810 125.2857 125.1387 2: methoxypiperidine ethyl ester: 1 cyprofen 1.3954 271.9005 116.8572 232.6777 4: 1: methoxypiperidine ethyl ester 2.3997 158.1073 110.1143 143.5847 8: 1: methoxypiperidine ethyl ester 1.9364 195.9358 105.6191 185.5118

[0038] As shown in Table 2, when methoxypiperidine ethyl ester and cyprofenoflavone are combined in a mass ratio of 1-8:15-1, the co-toxicity coefficient against papaya mealybug is greater than 120, indicating a synergistic effect.

[0039] Table 3. Results of in vitro bioactivity assays of the combined methoxypiperidine and fluazinam on Papaver somniferum mealybug.

[0040] Drug Name and Proportion <![CDATA[LD 50 (mg / L)]]> ATI TTI CTC Ethyl methoxypiperidine 3.7941 100.0000 -- -- Fluazinam 6.1222 61.9728 -- -- 1:10 methoxypiperidine ethyl ester: 1 fluoridamine 4.4085 86.0633 65.4298 131.5352 Ethyl methoxypiperidine 1: Fluazinam 5 3.7229 101.9125 68.3107 149.1897 Methoxypiperidine ethyl ester 1: Fluazinam 1 3.2230 117.7195 80.9864 145.3571 5:1 methoxypiperidine ethyl ester: 1 fluazinam 0.8241 460.3932 93.6621 491.5467 10 methoxypiperidine ethyl ester: 1 fluazinam 0.6132 618.7378 96.5430 640.8936 Ethyl methoxypiperidine 20: Fluazinam 1 2.6390 143.7704 98.1892 146.4218

[0041] As shown in Table 3, when methoxypiperidine ethyl ester and fluazinam are combined in a mass ratio of 1-20:10-1, the co-toxicity coefficient against papaya mealybug is greater than 120, indicating a synergistic effect.

[0042] In summary, this invention combines and screens methoxypiperidine ethyl ester with other insecticidal active ingredients, which can improve the control effect against papaya mealybug compared with single active ingredients. This provides a reference for the scientific and rational use of pesticides to control the newly invasive papaya mealybug and for the formulation of scientific and effective control measures.

[0043] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A pesticide composition for controlling papaya mealybug, characterized in that, Its active ingredient is a compound of methoxypiperidine ethyl ester and cyprofenoflavone, wherein the mass ratio of methoxypiperidine ethyl ester to cyprofenoflavone is 1-8:15-1.

2. An insecticide for controlling papaya mealybug, characterized in that, It includes the pesticide composition for controlling papaya mealybug as described in claim 1 and pesticide auxiliary ingredients.

3. The insecticide for controlling papaya mealybug according to claim 2, characterized in that, The pesticide auxiliary ingredients are selected from one or more of the following: wetting agents, dispersants, emulsifiers, solvents, cosolvents, inert fillers, disintegrants, thickeners, film-forming agents, defoamers, antifreeze agents, preservatives, and colorants.

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