High tolerance of glyphosate of esps synthase and its cloning, expression and application

By screening and genetically modifying marine bacteria, a glyphosate-tolerant EPSP synthase was developed, solving the problem of insufficient tolerance in existing glyphosate-resistant crops. This achieved high tolerance of transgenic plants to glyphosate, reducing herbicide usage and increasing crop yields and economic benefits.

CN117844776BActive Publication Date: 2026-08-25HUAZHONG AGRI UNIV
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Patent Information

Application Number
CN202410065456.X
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-01-16
Publication Date
2026-08-25
Estimated Expiration
2044-01-16

AI Technical Summary

Technical Problem

Existing glyphosate-resistant crops have insufficient glyphosate tolerance, leading to increased herbicide use and higher costs, which in turn affects crop yields and profits.

Method used

EPSP synthase with high glyphosate tolerance was screened from marine bacteria. Through gene mutation and modification, EPSP synthase with high glyphosate tolerance and its encoding gene were developed and transferred into plants to cultivate new transgenic crops.

Benefits of technology

It improved plant tolerance to glyphosate, enabling transgenic plants to tolerate four times the commercially recommended dose of glyphosate, reducing herbicide use and increasing crop yields and economic benefits.

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Abstract

The application belongs to the technical field of genetic engineering, and particularly relates to a novel EPSP synthase (5-enolpyruvylshikimate-3-phosphate synthase) mutant with high glyphosate tolerance, and further discloses application of a coding gene thereof in the field of novel glyphosate-tolerant crop cultivation. A 5-enolpyruvylshikimate-3-phosphate synthase is cloned from a marine strain Halomonas sp., and then through three rounds of directed evolution technology, multiple mutants with significantly improved glyphosate tolerance are obtained. The mutant coding gene is transferred into tobacco, and the tobacco is endowed with high resistance to herbicides, which proves that the mutant has high application value in the cultivation of herbicide-tolerant plants.
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