A method for promoting flesh color change in melon

Abstract

The present application belongs to the technical field of agricultural planting, and particularly relates to a method for promoting the color change of melon pulp. The present application provides a method for promoting the color change of melon pulp, comprising the following steps: at the bud stage or the flowering stage, applying forchlorfenprop and gibberellin to melon ovary; the concentration of the forchlorfenprop and the gibberellin is 5-20 mg / L respectively. By limiting the bud stage or the flowering stage to spray forchlorfenprop and gibberellin with specific concentration, not only the fruit setting rate is increased, but also the color change efficiency of orange pulp melon in the fruit development process is greatly improved. Experiments prove that, after the technical scheme provided by the present application is adopted, the color change time of the pulp in the fruit development process of orange pulp melon is obviously advanced, the orange degree of the pulp is deepened, and the commodity nature of the fruit is improved, thereby providing favorable conditions for the early picking and marketing of melon.

Description

Technical Field

[0001] This invention belongs to the technical field of agricultural planting, and specifically relates to a method for promoting the color change of melon flesh. Background Technology

[0002] Melon is an important economic crop, ranking among the world's top ten fruits. my country is the world's largest producer and consumer of melons. With the continuous improvement of people's living standards, the production and consumption of melons are also increasing year by year.

[0003] Currently, a plant growth regulator (chlorpyrifos, or CPPU) is widely used in melon production, especially under greenhouse cultivation conditions. Chlorpyrifos is a synthetic cytokinin that stimulates cell division and expansion, effectively inducing parthenocarpy and promoting fruit enlargement. However, while CPPU is simple to use, has high fruit-setting efficiency, eliminates the need for artificial pollination, reduces costs, and solves the problem of insufficient or poorly developed pollen in early spring greenhouse production, its use also has some adverse effects on melon quality. Specifically, it significantly affects the development of orange-fleshed melon color, mainly by reducing the intensity of the orange color and delaying the color change time, thus impacting the marketability of orange-fleshed melons. Summary of the Invention

[0004] The purpose of this invention is to provide a method for promoting the color change of melon flesh. This method can not only advance the color change time of melon flesh, but also increase the color intensity of orange-fleshed melons and improve the marketability of melons.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] This invention provides a method for promoting color change in melon flesh, comprising the following steps:

[0007] Apply chlorpyrifos and gibberellin to melons during the budding or flowering stage;

[0008] The concentrations of chlorpyrifos and gibberellin are 5–20 mg / L, respectively.

[0009] Preferably, the budding period includes the day before the ovary blooms;

[0010] The flowering period includes the day the ovary opens.

[0011] Preferably, the method of applying the chlorpyrifos and gibberellin includes: applying the chlorpyrifos and gibberellin in combination to the melon seed ovary or applying the chlorpyrifos and gibberellin separately to the melon seed ovary.

[0012] Preferably, promoting the color change of melon flesh includes: advancing the color change time of the flesh and / or deepening the color of the flesh.

[0013] The preferred method is to promote the color change of the flesh during the fruit development process of melons.

[0014] Preferably, the melon includes orange-fleshed melon.

[0015] Preferably, the varieties of orange-fleshed melon include: Dongfangmi No. 3 and / or Dongfangmi No. 1.

[0016] Beneficial effects:

[0017] This invention provides a method for promoting the color change of melon flesh, comprising the following steps: applying chlorpyrifos and gibberellin to the melon ovary during the budding or flowering stage; wherein the concentrations of chlorpyrifos and gibberellin are 10 mg / L respectively. By limiting the application of specific concentrations of chlorpyrifos and gibberellin during the budding or flowering stage, this invention not only increases the fruit set rate but also significantly improves the efficiency of flesh color change during the development of orange-fleshed melons. Experiments have shown that using the technical solution provided by this invention not only advances the time of flesh color change during the development of orange-fleshed melons but also deepens the flesh color, improving the marketability of the fruit and providing favorable conditions for early harvesting and marketing of melons. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the embodiments will be briefly described below.

[0019] Figure 1 The effects of different treatments in Example 1 and Comparative Example 1 on the size and sensory quality of melon fruits;

[0020] Figure 2 The effects of different treatments in Example 1 and Comparative Example 1 on the color of melon flesh at different stages are shown. Detailed Implementation

[0021] This invention provides a method for promoting color change in melon flesh, comprising the following steps: applying chlorpyrifos and gibberellin to the melon ovary during the budding or flowering stage; wherein the concentrations of chlorpyrifos and gibberellin are 10 mg / L respectively.

[0022] Unless otherwise specified, all reagents used in this invention are commercially available.

[0023] The present invention preferably prepares chlorpyrifos and gibberellin. In this invention, the concentrations of gibberellin and chlorpyrifos are preferably the same; the concentration of chlorpyrifos is 5–20 mg / L, preferably 10 mg / L; the concentration of gibberellin is 5–20 mg / L, preferably 10 mg / L; the preparation method is not particularly required and can be any technique well known in the art.

[0024] After obtaining the chlorpyrifos and gibberellin, the present invention applies the chlorpyrifos and gibberellin to the melon ovary. In the present invention, the preferred method of applying the chlorpyrifos and gibberellin includes: applying the chlorpyrifos and gibberellin in combination to the melon ovary or applying the chlorpyrifos and gibberellin separately to the melon ovary.

[0025] When chlorpyrifos and gibberellin are applied separately to melon ovaries, there are no special requirements for the spraying order of the chlorpyrifos solution and the gibberellin solution; when chlorpyrifos and gibberellin are combined and applied to melon ovaries, there are no special requirements for the mixing method, as long as the effective concentrations of chlorpyrifos and gibberellin in the mixture are the same and are 5 to 20 mg / L respectively.

[0026] The application time of this invention is during the bud stage or flowering stage, with the bud stage preferably being 1 day before the ovary blooms; the flowering stage preferably being the day the ovary blooms; the application site is preferably the surface of the ovary of the melon; there are no special requirements for the amount applied, as long as the surface of the ovary is completely moistened.

[0027] The melons described in this invention preferably include orange-fleshed melons; the varieties of orange-fleshed melons preferably include Dongfangmi No. 3 and / or Dongfangmi No. 1, more preferably Dongfangmi No. 3. The method of promoting melon flesh color change in this invention preferably includes advancing the color change time of the flesh and / or deepening the color of the flesh; the method of promoting melon color change in this invention preferably includes promoting flesh color change during the fruit development process of melons, more preferably promoting flesh color change 25-35 days after flowering; the color change preferably includes promoting the transformation of the flesh of orange-fleshed melons to orange.

[0028] Experiments have shown that the technical solution provided by this invention can mitigate the impact of CPPU spraying on the color change of melon flesh, improve the efficiency of flesh color change in orange-fleshed melons during fruit development, enhance the marketability of the fruit, and facilitate early harvesting and marketing of melons.

[0029] To further illustrate the present invention, the method for promoting color change in melon flesh provided by the present invention will be described in detail below with reference to the accompanying drawings and embodiments, but these should not be construed as limiting the scope of protection of the present invention.

[0030] Preparation before the experiment:

[0031] Preparation method of 20 mg / L CPPU solution (1L): Take 20 mL of 0.1% chlorpyrifos soluble concentrate (manufacturer: Sichuan Shite Optimization Chemical Co., Ltd., pesticide registration certificate number: PD20070131), dilute it 50 times, that is, add it to 980 mL of distilled water, stir and mix well to prepare 1 L of CPPU solution with a concentration of 20 mg / L.

[0032] Preparation method of 1.8L of 20mg / L gibberellin (GA) solution: Take 2mL of benzyl gibberellin soluble concentrate (manufacturer: Sichuan Runer Technology Co., Ltd., pesticide registration certificate number: PD20131024) with a total active ingredient of 1.8% (active ingredients are gibberellin A4 and A7), dilute it 900 times, and add it to 1798mL of distilled water. Stir and mix well to prepare 1.8L of gibberellin solution with a concentration of 20mg / L.

[0033] Preparation of a mixture containing CPPU and GA: Taking the preparation of 1L as an example, the preparation method is as follows: Take 500mL of the above-mentioned CPPU solution with a concentration of 20mg / L, add an equal volume of 500mL of the above-mentioned gibberellin (GA) solution with a concentration of 20mg / L, mix well, and you will get 1L of mixture. The concentration of CPPU in the mixture is 10mg / L, and the concentration of GA in the mixture is 10mg / L.

[0034] Example 1

[0035] A method to promote color change in melon flesh, comprising the following steps:

[0036] Experimental materials: 'Dongfangmi No. 3' (Cucumis melo L.), a thick-skinned cantaloupe variety;

[0037] Experiment location: Inside the greenhouse of the Shanghai Academy of Agricultural Sciences' Grain and Vegetable Research Base;

[0038] Experimental setup: Melon plants were grown on trellises with single-vine training, a plant spacing of 0.4m × 1.2m, fruit set at the 15th to 18th node, 2 fruits per plant, and the growing tip was pinched off when the plant height reached about 1.8m.

[0039] One day before or on the day of flowering, spray a mixture of CPPU and GA (the concentration of CPPU and GA in the mixture is 10 mg / L) evenly on the surface of the ovary until the surface of the ovary is completely wet. Set up three parallel experiments, with 30 melon plants planted in each parallel experiment. The rest of the time, the plants are managed in accordance with the normal management. The whole experiment is recorded as the C+GA group.

[0040] Comparative Example 1

[0041] The difference from Example 1 is that the mixture containing CPPU and GA in Example 1 was replaced with a 10 mg / L CPPU solution. The entire experiment was recorded as Group C.

[0042] When the melon fruits in Example 1 and Comparative Example 1 reached 20, 25, 30, 35, and 40 DAA (fruit maturity, DAA represents the number of days after flowering), three fruits from each parallel experiment of Group C (Comparative Example 1) and Group C+GA (Example 1) were taken. The fruits were transversely cut along the equator, and four portions of the pulp were selected from each cross section using a cross-sectional method. The color of the melon pulp was measured using a colorimeter (model: NH310, Shenzhen Sanenshi Technology Co., Ltd.) and recorded as CIE L*, a*, and b*, respectively representing dark (0) / light (100), red (+) / green (-), and yellow (+) / blue (-) scale values. The longitudinal diameter, transverse diameter, and weight of the melon fruits were also recorded. When the fruits reached maturity (40 DAA), the soluble solids content and pulp firmness of the fruits in Group C and Group C+GA were measured. Each group was repeated three times. The results are shown in Table 1. Figure 1 and Figure 2 (exist Figure 2 Note: ** indicates extremely significant difference from the control group (P<0.01); *** indicates very significant difference from the control group (P<0.001); Figure A shows cross-sectional views of the fruit of 'Dongfangmi No. 3' melon at different developmental stages, and Figure B shows the L*, a*, and b* values ​​of the pulp of the cross-section of 'Dongfangmi No. 3' melon at different developmental stages.

[0043] Table 1. Effects of different treatments on fruit size and sensory quality of melon.

[0044]

[0045]

[0046] Note:

[0047] 1. Superscript letters a and b indicate significant differences (p≤0.05);

[0048] 2. L*, a*, and b* represent the scale values ​​for dark (0) / bright (100), red (+) / green (-), and yellow (+) / blue (-), respectively.

[0049] From Table 1, Figure 1 and Figure 2It can be seen that there were no significant differences in fruit weight, longitudinal diameter, and transverse diameter between group C and group C+GA at 20, 25, 30, 35, and 40 DAA (days after flowering); at fruit maturity (40 DAA), there were no significant differences in soluble solids content (center and edge) and flesh firmness between the two groups; and at 20 DAA, there were no significant differences in flesh color and measured CIE L*, a*, and b* values ​​between the two groups, with the flesh being white with a slight green tinge. However, when the fruit developed to 25, 30, and 35 DAA, the CIE a* and b* values ​​of the flesh measured in the C+GA group were significantly higher than those in the C group, reaching highly significant levels except for the b* value at 25 DAA. The orange color of the flesh in the C+GA group was significantly deeper than that in the control group (C group). At 40 DAA, when the fruit was fully mature, the CIE a* and b* values ​​of the flesh measured in the C+GA group were slightly higher than those in the C group, but the difference was not significant. The flesh color of the two groups of fruits was similar, both being orange, and the difference in orange color intensity decreased. Therefore, this invention can significantly promote the speed and degree of orange flesh color change in the 'Dongfangmi 3' melon during fruit development while ensuring that CPPU promotes fruit enlargement.

[0050] In summary, this invention can significantly accelerate the speed and extent of orange flesh color change in the 'Dongfangmi No. 3' melon during fruit development. In actual melon production, especially in early spring, growers often harvest and market early to seize market opportunities and maximize profits. This frequently results in 'Dongfangmi No. 3' melons with orange flesh exhibiting "white flesh" or "red and white inlay," severely impacting melon quality and consumer purchasing power. The technical solution provided by this invention can significantly accelerate the speed and extent of orange flesh color change during fruit development, effectively solving or greatly alleviating the aforementioned problems and providing strong technical support for the demonstration and promotion of new melon varieties.

[0051] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A method for promoting color change in melon flesh, characterized in that, Includes the following steps: During the budding or flowering stage, apply chlorpyrifos and gibberellin to melons; the method of application is as follows: apply chlorpyrifos and gibberellin together to the melon ovary or apply chlorpyrifos and gibberellin separately to the melon ovary; The concentrations of chlorpyrifos and gibberellin are 5~20 mg / L, respectively; The melon in question is an orange-fleshed melon.

2. The method according to claim 1, characterized in that, The budding stage includes the day before the ovary blooms; The flowering period includes the day the ovary opens.

3. The method according to claim 1, characterized in that, The promotion of melon flesh color change includes: advancing the color change time of the flesh and / or deepening the color of the flesh.

4. The method according to claim 3, characterized in that, Promotes the color change of the flesh during the fruit development process of melons.

5. The method according to claim 1, characterized in that, The varieties of orange-fleshed melons include: Dongfangmi No. 3 and / or Dongfangmi No. 1.

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