Use of ZmCPK17 protein or its encoding gene in regulating plant cold resistance
By screening and validating overexpression lines of ZmCPK17 protein or its encoding gene, and using CRISPR/Cas9 technology to edit the ZmCPK17 gene to reduce its expression level, the sensitivity of maize to cold environments was resolved, and the cold resistance and growth performance of maize were improved.
CN118325965BActive Publication Date: 2026-06-16CHINA AGRI UNIV
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- CHINA AGRI UNIV
- Filing Date
- 2024-05-13
- Publication Date
- 2026-06-16
AI Technical Summary
⚠Technical Problem
Corn is sensitive to cold environments. The physiological mechanisms of corn cold resistance are not clear in current technologies, making it difficult to cultivate varieties with excellent cold resistance.
⚗Method used
By screening and validating overexpression lines of ZmCPK17 protein or its encoding gene, the expression level of ZmCPK17 protein was reduced, and the ZmCPK17 gene was edited in maize using CRISPR/Cas9 technology to regulate the plant's cold resistance.
🎯Benefits of technology
It significantly improved the plant's cold resistance, reduced damage under low-temperature stress, and enhanced the cold tolerance of maize.
✦ Generated by Eureka AI based on patent content.
Smart Images
Figure CN118325965B_ABST
Abstract
The application relates to the technical field of plant breeding, and particularly relates to application of a ZmCPK17 protein or a coding gene thereof in regulating cold resistance of plants. The ZmCPK17 protein can also be applied to cultivating plants with high cold resistance or high growth performance. The ZmCPK17 protein comprises an amino acid sequence as shown in SEQ ID NO. 1. A ZmCPK17 protein related to plant cold resistance is screened in the application, and the cold resistance of plants can be regulated by increasing or reducing the level of the ZmCPK17 protein in plants. The application of the ZmCPK17 protein provided in the application has important significance for the study of the cold tolerance molecular mechanism of corn, and lays a certain theoretical foundation for the study of the mechanism of plant response to low temperature stress and the molecular mechanism of resisting adverse environment.
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