Application of BnaHsfA2 gene and its overexpression vector in improving the frost resistance of rapeseed
By constructing the overexpression vector of the BnaHsfA2 gene in rapeseed and screening the interaction proteins BnaHsp70 and BnaMPK11, the problem of poor freezing resistance of rapeseed was solved, and the resistance of rapeseed to freezing stress was significantly improved.
Patent Information
- Application Number
- CN202410724046.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-05
- Publication Date
- 2025-03-25
- Estimated Expiration
- 2044-06-05
AI Technical Summary
Rapeseed has poor frost resistance under low temperature conditions, resulting in hindering overwintering and decreasing yield. The prior art has not yet clarified the molecular mechanism by which the Hsf gene regulates target genes in rapeseed to enhance frost resistance.
By constructing the overexpression vector of the BnaHsfA2 gene and transferring it into rapeseed, transgenic rapeseed was constructed to improve the resistance of rapeseed to freezing stress. At the same time, the BnaHsp70 and BnaMPK11 genes were screened to enhance the anti-freeze of rapeseed by upregulating its expression.
Through the overexpression of the BnaHsfA2 gene and the screening of interacting proteins, the anti-freeze of rapeseed was significantly improved, the accumulation of ROS was inhibited, the POD activity was improved, and the accumulation of MDA content was reduced, thereby enhancing the resistance of rapeseed to freezing stress.