Gene editing endonucleases with high PAM compatibility and their applications
By developing the endonuclease Gs12-19 with high PAM compatibility and its gene editing system, combined with LAMP technology, the problem of difficulty in early detection of cat infectious peritonitis virus in the existing technology is solved, and low-cost, field-based and efficient detection is achieved.
CN118755698BActive Publication Date: 2025-03-25HUAZHONG AGRI UNIV +1
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Patent Information
- Application Number
- CN202410790085.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-18
- Publication Date
- 2025-03-25
- Estimated Expiration
- 2044-06-18
AI Technical Summary
Technical Problem
The prior art is difficult to detect feline infectious peritonitis virus (FIPV) in the early stage, and the existing diagnostic methods have the disadvantages of long cycles and expensive equipment.
Method used
A new gene editing system mediated by Gs12-19, an endonuclease with high PAM compatibility was developed, and combined with LAMP technology, a nucleic acid visual detection technology based on Gs12-19 nuclease was established.
Benefits of technology
It realizes low-cost, on-site FIPV nucleic acid testing, which can early diagnosis of infectious peritonitis in cats, and improves the sensitivity and specificity of the detection.
✦ Generated by Eureka AI based on patent content.
Abstract
The present invention discloses a novel endonuclease Gs12-19 of the CRISPR / Cas system and its application. Specifically, the present invention provides a novel guide RNA-dependent endonuclease mined based on a metagenomics method. Studies have found that the PAM of Gs12-19 is NYYV (N is T / C / G / A base, Y is T / C base, and V is G / A / C base), which has the advantage of having a genome editing capability with better PAM compatibility, and can achieve high activity and specific cutting of target DNA in the genome. The present invention establishes a nucleic acid visualization detection technology mediated by the CRISPR / Gs12-19 system, which has broad application prospects in the field of genome site-specific modification and nucleic acid detection.
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