Molecular marker Ms_Chr2_65748048 related to plant height regeneration ability of alfalfa after mowing and its application
By discovering and applying the InDel molecular marker Ms_Chr2_65748048 on alfalfa chromosome 2, the problem of identifying plant height regeneration ability after alfalfa is cut is solved, and accurate identification and efficient screening of plant height regeneration ability is achieved.
CN118853941BActive Publication Date: 2025-03-21LANZHOU UNIV
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Patent Information
- Application Number
- CN202411134433.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-19
- Publication Date
- 2025-03-21
- Estimated Expiration
- 2044-08-19
AI Technical Summary
Technical Problem
The prior art is difficult to effectively identify and identify gene markers related to the regeneration ability of alfalfa after cutting, which affects the improvement of the high yield and regeneration ability of alfalfa.
Method used
A InDel molecular marker Ms_Chr2_65748048 located on alfalfa chromosome 2 was developed, and corresponding primer pairs were provided for PCR detection. Through this marker, the strength of the regeneration ability of the plant after alfalfa was cut can be quickly identified.
Benefits of technology
The accurate identification of the regeneration ability of the plant after alfalfa is cut is achieved, the breeding process is simplified, and the screening efficiency of high yield and regeneration ability is improved.
✦ Generated by Eureka AI based on patent content.
Abstract
The present invention provides an InDel molecular marker Ms_Chr2_65748048 related to the plant height regeneration ability of alfalfa after mowing and its application, belonging to the technical field of molecular markers. The present invention reflects the ability of alfalfa plants to regenerate within a certain period of time after mowing by measuring the plant height of alfalfa regenerated two weeks after mowing. The InDel molecular marker is located on chromosome 2 of alfalfa, and the nucleotide sequence of the inserted or deleted fragment is shown in the sequence table SEQ ID NO.3. The primer pair used to amplify the InDel molecular marker has a nucleotide sequence shown in SEQ ID NO.1-2. The InDel molecular marker and the primer pair described in the present invention can identify or assist in identifying the plant height regeneration ability of alfalfa after mowing. At the same time, the present invention also provides a method for quickly identifying whether alfalfa has the plant height regeneration ability after mowing by using the InDel molecular marker. The method is simple and fast, the identification result is accurate, and it has a good prospect for promotion and application.
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