A magnetic nanomaterial for antibody purification, preparation method and application thereof

By preparing Fe3O4@SiO2@APTMS@PIDA-Ni2+ magnetic nanomaterials, the problems of insufficient biocompatibility, stability and selectivity of cured carriers in existing antibody purification technologies are solved, and efficient and reversible protein immobilization and antibody purification effects are achieved.

CN118955696BActive Publication Date: 2025-03-21ZHEJIANG UNIV OF SCI & TECH
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Patent Information

Application Number
CN202411437987.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-10-15
Publication Date
2025-03-21
Estimated Expiration
2044-10-15

AI Technical Summary

Technical Problem

In the existing antibody purification technology, the biocompatibility, stability and selectivity of the curing carrier are insufficient, and blocking of protein active sites is difficult to avoid.

Method used

By preparing Fe3O4@SiO2@APTMS@PIDA-Ni2+ magnetic nanomaterial, the material modified the long chain bicarboxylic structure on the surface, bound Ni2+, to specifically bind to the histidine tag on mycoplasma protein, achieving high selectivity and reversible binding ability.

Benefits of technology

This magnetic nanomaterial has high biocompatibility, stability and selectivity, can effectively immobilize protein M without destroying its active site, and can be reused, suitable for the purification and rapid detection of antibodies.

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Abstract

The present application relates to the field of mycoplasma protein immobilization, and in particular to a magnetic nanomaterial for mycoplasma protein immobilization, a preparation method and its application. A magnetic nanomaterial for mycoplasma protein immobilization is prepared by reacting the following compounds by weight: ferric chloride hexahydrate (FeCl3·6H2O), ferrous chloride tetrahydrate (FeCl2·4H2O), ammonia water (NH3·H2O), (3-aminopropyl) trimethoxysilane (APTMS), tetraethyl silicate, sodium chloroacetate, triethylamine and nickel sulfate hexahydrate (NiSO4·6H2O). The magnetic nanomaterial has the advantages of strong ability to load mycoplasma protein, no toxicity to mycoplasma protein, and firm adsorption of protein on the surface of the magnetic nanomaterial.
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