Detection method of phenyl content in polysaccharide microspheres

Abstract

The present application relates to the technical field of polysaccharide analysis, and in particular to a method for detecting the phenyl content of polysaccharide microspheres coupled. The method comprises the following steps: mixing polysaccharide microspheres coupled with functional groups and a first acid agent to break the ether bond in the functional group to obtain a dissociation solution; mixing the dissociation solution and a first solvent to obtain a sample to be tested; mixing a standard substance and a second acid agent, and diluting the obtained mixed solution with a second solvent to prepare standard solutions of different concentrations; testing the absorbance values ​​of the standard solutions of different concentrations and the sample to be tested at a wavelength λ, and drawing a standard curve according to the concentration and absorbance values ​​of the standard solutions of different concentrations, and calculating the concentration of phenyl in the sample to be tested according to the standard curve. The above method can overcome the problem of the difficulty in quantitative detection of phenyl functional groups on polysaccharide microspheres, the structural characterization of polysaccharide microspheres, and the front-end structural design of polysaccharide microspheres, and the testing method is simple.