A method for monitoring fruit fly movement, sleep and circadian rhythms

By improving the size and conditions of the insect monitoring tube and combining the use of the LAM25 monitor, accurately monitoring the sleep and circadian rhythm of fruit fly, the problem of the inability to accurately monitor the circadian rhythm of fruit fly in the prior art has been solved, and a more accurate monitoring effect has been achieved.

CN119096947BActive Publication Date: 2025-03-28BEE RES INST CHINESE ACAD OF AGRI SCI
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Patent Information

Application Number
CN202411448937.5
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-10-17
Publication Date
2025-03-28
Estimated Expiration
2044-10-17

AI Technical Summary

Technical Problem

Existing LAM25 monitors cannot accurately monitor the circadian rhythm of fruit flies, and accurate movement and sleep information cannot be obtained for insects with less exercise.

Method used

By improving the size and conditions of the insect monitoring tube, the A, B, and C combination devices are used to place the fruit fly in an appropriate space and monitor it in the LAM25 monitor. Combined with data analysis under dark and light conditions, the sleep and circadian rhythm of the fruit fly are accurately monitored.

Benefits of technology

More accurate monitoring of fruit fly movement, sleep and circadian rhythms is achieved, improving the accuracy of monitoring and the authenticity of data.

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Abstract

The present invention relates to the field of insect behavior, and in particular to a method for real-time continuous monitoring of the movement, sleep and circadian rhythm of a dipteran insect, a fruit fly, comprising the following steps: S1: fruit fly food preparation; S2: take a double-end perforated acrylic tube A, add 2 cm of solid food at one end, put in the fruit fly, and plug the other end with a 2 cm cotton plug; S3: insert two double-end perforated acrylic tubes B into the acrylic double-opening tube A to form an A, B combination device; S4: screw the A, B combination device into the acrylic double-opening tube C to form an A, B, C combination device; S5: put the A, B, C combination device into a LAM25 monitor for monitoring; S6: analyze data through sleep and circadian rhythm analysis software. On the basis of the original insect behavior monitoring system, the present invention focuses on the fruit fly species as the research object, optimizes the monitoring device according to the monitoring data, and establishes an accurate method for monitoring the movement, sleep and circadian rhythm state of the fruit fly.
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