Modularly designed high-flux parallel chemical reaction device

Abstract

The invention discloses a high-flux parallel chemical reaction device with modular design. The device comprises a vertical magnetic stirring and mixing platform, a temperature control module, a sample reaction tube module, an electrochemical reaction module and a photochemical reaction module. According to the device, a vertical magnetic stirring and mixing platform adopting a vertical magnetic stirring and mixing technology is adopted as a basic platform of a reactor, and is combined with a temperature control module capable of performing a precise temperature control technology, a photochemical reaction module adopting a light source power control technology and an electrochemical reaction module adopting a direct / alternating current power supply control technology; a modular design technology is adopted, the device completely follows international SLAS / SBS laboratory automation specifications and standards, and high-throughput chemical experiments such as temperature control type chemical reactions, photochemical reactions, electrochemical reactions and the like can be conveniently, flexibly and freely combined.

Description

Technical Field

[0001] The invention relates to the field of laboratory technology, and in particular to a high-throughput parallel chemical reaction device with a modular design. Background Art

[0002] High-throughput experimental technology originated in the 1980s and has since developed rapidly in the field of biological science experimental technology. In particular, combined with the rapid development of laboratory automation technology, it has greatly promoted biological science research such as high-throughput gene sequencing, high-throughput cell screening, high-throughput nucleic acid virus purification, high-throughput synthetic biology, and high-throughput ELISA testing.

[0003] The basic principle of high-throughput experimental technology is to conduct multiple experiments (usually dozens, hundreds, or even thousands) simultaneously in a parallel processing manner to explore the relationship between various variable parameters. Today's high-throughput experimental technology is developing in the direction of "automation, miniaturization, and scale", hoping to obtain "the most data" at the "fastest speed, highest efficiency, and lowest cost". This goal is also closely integrated with today's scientific trends in artificial intelligence and machine learning. Specifically for chemical synthesis high-throughput technology, it is usually required to "conduct hundreds or thousands of parallel chemical reactions in 1-2 ml reaction tubes at the same time (such as 96 or 384-well plate mode)", so that the cost of a single chemical reaction can be minimized (the least reaction raw materials), and the chemical reaction is operated automatically, so the speed is greatly improved and the time cost is minimized.

[0004] High-throughput experimental methods based on multi-well plates (24 / 48 / 96 / 384-well plates) according to SLAS / SBS ​​standards are already commonplace in the field of biological high-throughput, and are low-cost and easy to implement, but they are extremely challenging in the field of chemical synthesis: First, chemical reaction conditions are much more intense than biological reactions. Most biological high-throughput experiments are mild experimental conditions. The 96 / 384-well plates used are usually biocompatible "plastic materials", which cannot meet the usual "high temperature and high pressure" experimental conditions of chemical reactions. Most chemical reaction high-throughput experiments are intense experimental conditions, requiring them to be carried out in "temperature-resistant and pressure-resistant" glass reaction tubes, and the glass reaction tubes need to be placed in aluminum metal blocks to meet the temperature control requirements of "fast and uniform heat conduction". Secondly, the physicochemical properties of chemical reaction reagent materials are also very different from those of biological reaction reagent materials. Biological reactions are usually carried out in aqueous biological reagents, and are characterized by "single homogeneous phase, low viscosity, easy mixing, and short reaction time"; the characteristics of chemical reactions are "multi-phase coexistence of solid and liquid, high viscosity, strong stirring and mixing, and long reaction time". Therefore, the "oscillation mixing and suction-spray mixing" commonly used in biological high-throughput experiments cannot meet the experimental requirements of high-throughput automation of chemical reactions.

[0005] Today's chemical reaction technology is far from reaching the level of true high-throughput experiments. It is still at the stage of "several parallel reactions carried out simultaneously" and is usually carried out at the experimental level of "reaction scale of tens to hundreds of milliliters". This is far from the goal of "modern high-throughput chemical experiments": first, the number of single experiments is too small, resulting in "slow speed and low efficiency"; second, the "single reaction scale is too large", resulting in "large consumption of raw materials for each reaction and high cost", all of which cannot meet the goal requirements of "low-cost big data" for large-scale machine learning.

[0006] Currently, technologies and devices that have the true meaning of "modular high-throughput chemical reaction devices" are still extremely rare. Similar parallel chemical reaction devices have more or less some problems. For example, the utility model patent with the authorization number CN 220371009U discloses "a photochemical parallel reactor device", which usually can only carry out less than 10 photochemical reaction experiments in parallel at a time, and the scale of a single reaction is "30ml reaction tube or above"; the utility model patent with the authorization number CN 215611455 U discloses "parallel reactor", which usually can only carry out 10 chemical reaction experiments in parallel at a time, and the scale of a single reaction is "25ml Schlenk reaction tube"; the utility model patent with the authorization number CN 111167361 ... A's invention patent can achieve "synchronous magnetic stirring of 0-200 small-volume liquids of 5ml to 20ml, many groups of stirring experiments can be done at one time, and precise and stable stirring can be performed, and precise gradient temperature control can be achieved, and the operating temperature range is wide (-20℃ to 100℃)", but this design is extremely complicated. Due to physical space limitations, 5ml glass tubes are already the limit, so it is impossible to achieve the laboratory high-throughput automation requirements of "96-well 1ml glass tube reaction blocks", and the huge volume is difficult to integrate into the automated experimental platform; for example, the invention patent with application publication number CN 118076439 A discloses an "integrated system for chemical, biochemical or molecular biological reactions in microplates" device, the content of which includes "uniform high-precision modular temperature control" technology, but it is only suitable for mild biochemical reactions, not for high-throughput chemical synthesis reactions. Summary of the invention

[0007] In order to realize a truly "modern high-throughput automated chemical reactor", the present invention provides a modularly designed high-throughput parallel chemical reaction device, which adopts modular design technology and fully complies with international SLAS / SBS ​​laboratory automation specifications and standards. It can conveniently and flexibly carry out high-throughput chemical experiments with various reaction scales and various reaction types (temperature-controlled chemical reactions, photochemical reactions, electrochemical reactions) in free combination.

[0008] The technical solution provided by the present invention is as follows:

[0009] The present invention provides a high-throughput parallel chemical reaction device with modular design, comprising a vertical magnetic stirring mixing platform, a temperature control module, and one or more of a sample reaction tube module, an electrochemical reaction module, and a photochemical reaction module arranged on the temperature control module;

[0010] The vertical magnetic stirring mixing platform has a built-in vertical magnetic force line generating module, and the vertical magnetic force line generating module is used to emit magnetic force lines vertical to the top of the vertical magnetic stirring mixing platform;

[0011] The temperature control module is arranged on the top of the vertical magnetic stirring mixing platform for temperature control;

[0012] The sample reaction tube module is arranged on the temperature control module and is used to fix the sample container;

[0013] The electrochemical reaction module is arranged on the temperature control module, and comprises an electrode module and an electrochemical reaction sample tube module. The electrochemical reaction sample tube module is uniformly stirred under the action of the magnetic lines provided by the vertical magnetic lines generating module, and the electrode module controls the sample in the electrochemical reaction sample tube module to perform a high-throughput chemical reaction.

[0014] The photochemical reaction module is arranged on the temperature control module, and includes a light source module and a photoreaction sample tube module. The photoreaction sample tube module is uniformly stirred under the action of the magnetic lines provided by the vertical magnetic line generating module, and the light source module controls the sample in the photoreaction sample tube module to perform a photochemical reaction.

[0015] Further, the electrode module includes electrodes, an electrode mounting substrate and an electrode control bus;

[0016] The electrochemical reaction sample tube module comprises a metal heat conductive block base;

[0017] The metal heat-conducting block base is arranged on the temperature control module, and a plurality of first reaction bottles are placed on the metal heat-conducting block base, and a strong magnetic stirring bar 1 is placed in each of the first reaction bottles;

[0018] The electrode mounting substrate is arranged on the top of the first reaction bottle corresponding to the metal heat conductive block base, one end of each of the electrodes is mounted on the electrode mounting substrate, and the other end extends into the corresponding first reaction bottle respectively, and the electrodes are energized through the electrode control bus.

[0019] Furthermore, the light source module includes a dot matrix light source board, a light source control bus and light source lamp beads;

[0020] The photoreaction sample tube module comprises a metal base;

[0021] The metal base is arranged on the dot matrix light source board, and a plurality of second reaction bottles are placed on the metal base. A strong magnetic stirrer 2 is placed in each of the second reaction bottles. A light source lamp bead is arranged on the dot matrix light source board at a position corresponding to each of the second reaction bottles, and the light source lamp bead is used to illuminate the chemical reagents in the second reaction bottles.

[0022] Furthermore, the metal base has a built-in microporous channel 1, and a cooling liquid inlet and outlet are provided on the side wall of the metal base, and the cooling liquid inlet and outlet are used to pass a heat-conducting or cooling-conducting fluid, and the heat-conducting or cooling-conducting fluid controls the temperature of the photochemical reaction in the second reaction bottle through the microporous channel 1;

[0023] The dot matrix light source board has a built-in microporous channel 2, and a cooling liquid inlet and outlet are arranged on the side wall of the dot matrix light source board. The cooling liquid inlet and outlet are used to pass cooling liquid, and the cooling liquid enters the microporous channel 2 to cool the light source lamp beads.

[0024] Furthermore, the temperature control module includes a temperature control base, and a plurality of the temperature control bases are located directly above the vertical magnetic line generating module and are arranged in sequence along the direction of the vertical magnetic line generating module, and the temperature control bases are connected to a temperature controller.

[0025] Furthermore, the outer dimensions of the sample reaction tube module, the metal heat conductive block base and the metal base are consistent with the well plate dimensions of the SLAS / SBS ​​standard, and the interior is designed to be 96 wells 1 ml, 48 wells 2 ml, 24 wells 4 ml or 8 ml.

[0026] Furthermore, the vertical magnetic field line generating module includes a magnetic pole group and a fixing frame;

[0027] The fixing frame is arranged outside the magnetic pole group and is used for fixing the magnetic pole group;

[0028] The magnetic pole set includes a connecting frame and one or more magnets;

[0029] The connecting frame is arranged inside the fixing frame and is parallel to the plane where the bottom surface of the temperature control module is located;

[0030] One magnet is fixed on the connecting frame alone; or a plurality of magnets are evenly and evenly fixed on the connecting frame at equal intervals, and a line connecting two magnetic poles of each magnet is perpendicular to the bottom surface of the temperature control module.

[0031] Furthermore, a driving module is also provided in the vertical magnetic stirring mixing platform, and the driving module is connected to the vertical magnetic force line generating module and is used to drive the vertical magnetic force line generating module to rotate in a plane perpendicular to the bottom surface of the temperature control module.

[0032] Furthermore, the driving module includes a driving motor, a coupling and a speed controller;

[0033] The output shaft of the driving motor is connected to the connecting frame, the speed controller is connected to the driving motor, and the connecting frame is connected to the output end of the driving motor through the coupling.

[0034] Furthermore, the vertical magnetic stirring mixing platform also includes a host control screen, and the host control screen is arranged on the front of the vertical magnetic stirring mixing platform;

[0035] The magnet is a neodymium iron boron magnet.

[0036] Compared with the prior art, the present invention has the following beneficial effects:

[0037] 1. The modular high-throughput parallel chemical reaction device provided by the present invention can be freely combined and modularly assembled as needed to carry out various high-throughput chemical reactions; the stirring and mixing of various types of chemical reactions are driven by the high-speed rotation of the vertical magnetic line generating module to drive all magnetic stirrers to rotate vertically with the horizontal line as the rotation axis, while the ordinary magnetic stirrer uses the vertical line as the rotation axis, so as to achieve the uniform stirring and mixing effect required for each reaction;

[0038] 2. The modular high-throughput parallel chemical reaction device provided by the present invention adopts "vertical magnetic stirring mixing technology" as the basic platform of the reactor, combines precision temperature control technology (heating or cooling), light source power control technology and DC / AC current power supply control technology, follows the modular design principle, and is based on the international laboratory automation SLAS / SBS ​​standard specifications. On one platform device, "high-throughput temperature-controlled chemical reactions, high-throughput photochemical reactions and high-throughput electrochemical reactions" can be carried out separately or in combination. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 A schematic diagram of the structure of a high-throughput parallel chemical reaction device with modular design provided by the present invention;

[0040] Figure 2 This is a schematic diagram of the structure of an electrochemical reaction module in an embodiment of the present invention;

[0041] Figure 3 is a cross-sectional view of an electrochemical reaction module in an embodiment of the present invention;

[0042] Figure 4This is a schematic diagram of the structure of a photochemical reaction module in an embodiment of the present invention;

[0043] Figure 5 is a cross-sectional view of a photochemical reaction module in an embodiment of the present invention;

[0044] Figure 6 Schematic diagram of the structure of the vertical magnetic line generating module and the driving module in the embodiment of the present invention.

[0045] The reference numerals are as follows:

[0046] 1-vertical magnetic stirring mixing platform, 101-host control screen, 102-fixed frame, 103-connecting frame, 104-magnet, 105-drive motor, 108-coupling, 2-temperature control module, 3-electrochemical reaction module, 301-metal heat conductive block base, 302-first reaction bottle, 303-strong magnetic stirring bar one, 304-electrode mounting substrate, 305-electrode, 306-electrode control bus, 307-first sealing cover, 4-photochemical reaction module, 401-metal base, 402-second reaction bottle, 403-lattice light source board, 404-strong magnetic stirring bar two, 405-cooling liquid inlet and outlet, 406-light source control bus, 407-second sealing cover, 408-light source lamp bead, 5-sample reaction tube module. DETAILED DESCRIPTION

[0047] In order to make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the embodiments described below are part of the embodiments of the present application, rather than all of the embodiments. The components of the embodiments of the present application described and shown in the drawings here can be arranged and designed in various different configurations.

[0048] Therefore, the detailed description of the embodiments of the present application provided below in conjunction with the accompanying drawings is intended to represent only selected embodiments of the present application, and is not intended to limit the scope of protection claimed in the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present application.

[0049] It should be understood that in the description of the embodiments of the present invention, the terms "first", "second", etc. are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first", "second", etc. may explicitly or implicitly include one or more of the features.

[0050] In the description of the embodiments of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or can communicate with each other; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the embodiments of the present invention can be understood according to specific circumstances.

[0051] See also Figure 1 The present invention provides a high-throughput parallel chemical reaction device with a modular design, which includes a vertical magnetic stirring mixing platform 1, a temperature control module 2, an electrochemical reaction module 3, a photochemical reaction module 4 and a sample reaction tube module 5.

[0052] The vertical magnetic stirring mixing platform 1 has a built-in vertical magnetic force line generating module, which is used to emit magnetic force lines on the top of the vertical magnetic stirring mixing platform 1. The built-in vertical magnetic force line generating module in the vertical magnetic stirring mixing platform 1 adopts the vertical magnetic stirring mixing technology, and the vertical magnetic stirring mixing platform 1 is also the basic platform of the device.

[0053] The temperature control module 2 is disposed on the top of the vertical magnetic stirring mixing platform 1 for temperature control.

[0054] The sample reaction tube module 5 is disposed on the temperature control module 2 and is used to fix the sample container.

[0055] The electrochemical reaction module 3 is arranged on the temperature control module 2, and includes an electrode module and an electrochemical reaction sample tube module. The electrochemical reaction sample tube module is uniformly stirred under the action of the magnetic lines provided by the vertical magnetic line generating module, and the electrode module controls the sample in the electrochemical reaction sample tube module to perform a high-throughput chemical reaction.

[0056] The photochemical reaction module 4 is arranged on the temperature control module 2, and includes a light source module and a photoreaction sample tube module. The photoreaction sample tube module is uniformly stirred under the action of the magnetic lines provided by the vertical magnetic line generating module, and the light source module controls the sample in the photoreaction sample tube module to perform a photochemical reaction.

[0057] It should be noted that the above-mentioned multiple modules can be combined arbitrarily as needed, and high-throughput chemical experiments of temperature-controlled chemical reactions, photochemical reactions, and electrochemical reactions can be conveniently and flexibly performed. Among them, temperature-controlled chemical reactions are performed by combining the temperature control module 2 with the sample reaction tube module 5, electrochemical reactions are performed by the electrochemical reaction module 3, and photochemical reactions are performed by the photochemical reaction module 4. Temperature-controlled chemical reactions, photochemical reactions, and electrochemical reactions can be performed simultaneously or only one or two of them can be performed as needed for high-throughput chemical experiments.

[0058] like Figure 2 , Figure 3 As shown, the electrode module includes an electrode 305 , an electrode mounting substrate 304 and an electrode control bus 306 .

[0059] The electrochemical reaction sample tube module includes a metal heat conductive block base 301 .

[0060] The metal heat-conducting block base 301 is arranged on the temperature control module 2 , and a plurality of first reaction bottles 302 are placed on the metal heat-conducting block base 301 , and a strong magnetic stirring bar 303 is placed in each first reaction bottle 302 .

[0061] The electrode mounting substrate 304 is arranged on the top of the first reaction bottle 302 corresponding to the metal heat conductive block base 301. One end of multiple electrodes 305 is mounted on the electrode mounting substrate 304, and the other end extends into the corresponding first reaction bottle 302 respectively. The electrodes 305 are connected to a multi-channel DC or AC power supply through the electrode control bus 306.

[0062] like Figure 4 , Figure 5 As shown, the light source module includes a dot matrix light source board 403 , a light source control bus 406 and light source lamp beads 408 .

[0063] The photoreaction sample tube module includes a metal base 401, which is arranged on a dot matrix light source plate 403. A plurality of second reaction bottles 402 are placed on the metal base 401. A strong magnetic stirrer 404 is placed in each second reaction bottle 402. A light source lamp bead 408 is arranged at a position corresponding to each second reaction bottle 402 on the dot matrix light source plate 403. The light source lamp bead 408 is used to illuminate the chemical reagents in the second reaction bottle 402.

[0064] Optionally, the metal base 401 has a built-in microporous channel 1, and a cooling liquid inlet and outlet 405 is set on the side wall of the metal base 401. The cooling liquid inlet and outlet 405 is used to pass a heat-conducting or cooling-conducting fluid. The heat-conducting or cooling-conducting fluid controls the temperature of the photochemical reaction in the second reaction bottle 402 through the microporous channel 1.

[0065] The dot matrix light source plate 403 has a built-in microporous channel 2, and a cooling liquid inlet and outlet 405 is provided on the side wall of the dot matrix light source plate 403. The cooling liquid inlet and outlet 405 is used to pass the cooling liquid, and the cooling liquid enters the microporous channel 2 to cool the light source lamp beads 408.

[0066] Specifically, the dot matrix light source board 403 of the light source module is Figure 4 , Figure 5 As shown, light source lamp beads 408 of various wavelengths are assembled, and the coolant enters the microporous channel 2 to cool the light source lamp beads 408 and take away the heat generated by the light source lamp beads 408 when working. Single or multiple light source lamp beads 408 are connected to an external power regulator as needed to achieve real-time adjustment and control. The bottom of the metal base 401 corresponds to the position of the second reaction bottle 402, and a highly transparent quartz glass or similar material is penetrated or set to ensure that the light source lamp beads 408 of the light source module can perform light reaction on the chemical reagents in the second reaction bottle 402. The microporous channel 1 built into the light reaction sample tube module can pass heat-conducting or cooling-conducting fluids to ensure precise temperature control of the photochemical reaction.

[0067] Optionally, the temperature control module 2 includes a temperature control base, and multiple temperature control bases are located directly above the vertical magnetic line generating module and are arranged in sequence along the direction of the vertical magnetic line generating module, and the temperature control bases are connected to the temperature controller.

[0068] It should be noted that the temperature control module 2 used in the present invention can perform temperature-controlled chemical reactions, and can also heat or cool the electrochemical reaction module 3 and the photochemical reaction module 4. The temperature control module 2 adopts the existing technology, and it can be heated by the principle of resistance wire heating. When cooling is required, ice water can be introduced into the temperature control module 2 to achieve the cooling effect. When the temperature control module 2 used can perform temperature-controlled chemical reactions, it is necessary to place the sample reaction tube module 5 on the temperature control module 2, and then install the sample tube in the corresponding position in the sample reaction tube module 5, and a strong magnetic stirrer three (not shown in the figure) is also placed in the sample tube.

[0069] Optionally, the outer dimensions of the sample reaction tube module 5, the metal heat conductive block base 301 and the metal base 401 are consistent with the SLAS / SBS ​​standard well plate dimensions, and the interiors thereof are designed to be 96 wells 1 ml, 48 wells 2 ml, 24 wells 4 ml or 8 ml in specifications.

[0070] Specifically, the sample reaction tube module 5, the metal heat conductive block base 301 and the metal base 401 are made of metal aluminum blocks or other metal materials with excellent thermal conductivity, and their outer dimensions are the SLAS / SBS ​​standard well plate size (about 127.6mm x 85.4mm) to ensure compatibility with the commercial platform. The metal heat conductive block base 301 and the metal base 401 are processed into a 96-well plate that can accommodate 1ml glass reaction tubes, a 48-well plate that can accommodate 2ml glass reaction tubes, a 24-well plate that can accommodate 4ml or 8ml glass reaction tubes (with the same diameter), etc. as required. Each reaction tube is built with a micro stirring magnet of appropriate size. Under the strong drive of the vertical rotating magnetic field of the vertical magnetic line generating module in the vertical magnetic stirring mixing platform 1, the magnets in the reaction tube are driven to rotate to achieve the effect of stirring and mixing. Figure 2 and Figure 4 Shown is a diagram of a 24-well plate structure that accommodates 4 ml or 8 ml glass reaction tubes (same diameter).

[0071] like Figure 6 As shown, the vertical magnetic field line generating module includes a magnetic pole group and a fixing frame 102. The fixing frame 102 is arranged outside the magnetic pole group and is used to fix the magnetic pole group. The magnetic pole group includes a connecting frame 103 and one or more magnets 104. The connecting frame 103 is arranged inside the fixing frame 102 and is parallel to the plane where the bottom surface of the temperature control module 2 is located.

[0072] If the number of the magnet 104 is one, one magnet 104 is fixed on the connecting frame 103 alone.

[0073] If there are multiple magnets 104 , the multiple magnets 104 are evenly fixed on the connecting frame 103 at equal intervals, and the connecting line between the two magnetic poles of each magnet 104 is perpendicular to the bottom surface of the temperature control module 2 .

[0074] Optionally, a driving module is further provided in the vertical magnetic stirring mixing platform 1 , and the driving module is connected to the vertical magnetic force line generating module and is used to drive the vertical magnetic force line generating module to rotate in the plane of the bottom surface of the vertical temperature control module 2 .

[0075] Optionally, the driving module includes a driving motor 105, a coupling 108 and a speed controller.

[0076] The output shaft of the driving motor 105 is connected to the connecting frame 103 , the speed controller is connected to the driving motor 105 , and the connecting frame 103 is connected to the output end of the driving motor 105 via a coupling 108 .

[0077] Optionally, the vertical magnetic stirring mixing platform 1 further includes a host control screen 101 , and the host control screen 101 is arranged on the front side of the vertical magnetic stirring mixing platform 1 .

[0078] In this embodiment, the host control screen 101 is connected to the drive motor 105 to adjust the speed of the drive motor 105, so that the strong magnetic stirrer 1 303 and the strong magnetic stirrer 2 404 are suspended in the sample. The drive motor 7 can be a horizontally arranged rotary motor. In actual use, the speed of the drive motor 105 can be adjusted by the host control screen 101 according to different sample viscosities.

[0079] The vertical magnetic stirring mixing platform 1 uses neodymium iron boron magnets with high magnetic energy product and good high temperature performance as the poles of the magnetic pole group, and maintains the distance between the drive motor 105 and the magnetic pole group through the coupling 1088, which can effectively avoid the interference of the magnetic field of the drive motor 105 on the magnetic lines of force.

[0080] In order to achieve a better sealing effect and prevent the sample in the reaction bottle from leaking out from the bottle mouth, a first sealing cover 307 and a second sealing cover 407 may be provided at the bottle mouth of the first reaction bottle 302 and the second reaction bottle 402 respectively.

[0081] The modularly designed high-throughput parallel chemical reaction device provided by the present invention can be freely combined and modularly assembled as needed to carry out various types of high-throughput chemical reactions. The stirring and mixing of various types of chemical reactions are driven by the high-speed rotation of the vertical magnetic line generating module in the vertical magnetic stirring and mixing platform 1 to drive all magnetic stirrers to rotate vertically with the horizontal line as the rotation axis, while the ordinary magnetic stirrer uses the vertical line as the rotation axis to achieve the uniform stirring and mixing effect required for each reaction; the temperature, light, and current required for each type of chemical reaction are provided and executed by their respective functional modules. In addition, the device of the present invention can be integrated with the automation platform to realize automatic feeding, sampling, detection and other automated operations for each reaction tube, realizing truly fully automatic high-throughput chemical reactions, and the modular design based on the SLAS / SBS ​​standard specifications is also easy to realize automated integration.

[0082] In this field, the realization principle of all magnetic stirring devices is the same, and all of them form a changing magnetic field by rotating the driving magnet. The stirring magnet is subjected to the attraction-repulsion force in the changing magnetic field and rotates with the magnetic field, thereby realizing the stirring and mixing function. However, in this scheme, the "vertical magnetic stirring and mixing technology" is adopted. The vertical magnetic field generating module will generate a vertical magnetic field. When the driving module controls the vertical magnetic field generating module to rotate in the vertical direction, the magnetic field will rotate with the rotation of the vertical magnetic field generating module, and a driving field of vertical rotating magnetic field lines can be formed outside the vertical magnetic field generating module. When the magneton is located in the driving field, the magneton will rotate with the rotation of the magnetic field line. The macroscopic embodiment of this process is that the magneton rotates the same as the vertical magnetic field generating module in the driving field.

[0083] The present invention adopts a unique "vertical magnetic stirring mixing technology" as the basic platform of the reactor, combined with precise temperature control technology (heating or cooling), light source power control technology and DC / AC current power supply control technology, in accordance with the modular design principle, based on the international laboratory automation SLAS / SBS ​​standard specifications, on a platform device, "high-throughput temperature-controlled chemical reactions, high-throughput photochemical reactions and high-throughput electrochemical reactions" can be carried out separately or mixed.

[0084] The modularly designed high-throughput parallel chemical reaction device provided by the present invention adopts modular design technology and fully complies with the international SLAS / SBS ​​laboratory automation specifications and standards. It can conveniently and flexibly carry out high-throughput chemical experiments with various reaction scales and various reaction types (temperature-controlled chemical reactions, photochemical reactions, electrochemical reactions) in free combination.

[0085] The above is only the best specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions within the technical scope disclosed in the present application shall be included in the protection scope of the present application. Therefore, the protection scope of the present application shall be based on the protection scope of the claims.

Claims

1. A modular high-throughput parallel chemical reaction device, characterized in that: It includes a vertical magnetic stirring mixing platform, a temperature control module, and one or more of a sample reaction tube module, an electrochemical reaction module, and a photochemical reaction module arranged on the temperature control module; The vertical magnetic stirring mixing platform has a built-in vertical magnetic force line generating module, and the vertical magnetic force line generating module is used to emit magnetic force lines vertical to the top of the vertical magnetic stirring mixing platform; The temperature control module is arranged on the top of the vertical magnetic stirring mixing platform for temperature control; The sample reaction tube module is arranged on the temperature control module and is used to fix the sample container; The electrochemical reaction module is arranged on the temperature control module, and comprises an electrode module and an electrochemical reaction sample tube module. The electrochemical reaction sample tube module is uniformly stirred under the action of the magnetic lines provided by the vertical magnetic lines generating module, and the electrode module controls the sample in the electrochemical reaction sample tube module to perform a high-throughput chemical reaction. The photochemical reaction module is arranged on the temperature control module, and includes a light source module and a photoreaction sample tube module. The photoreaction sample tube module is uniformly stirred under the action of the magnetic lines provided by the vertical magnetic line generating module, and the light source module controls the sample in the photoreaction sample tube module to perform a photochemical reaction.

2. The modular high-throughput parallel chemical reaction device according to claim 1, characterized in that: The electrode module includes an electrode, an electrode mounting substrate and an electrode control bus; The electrochemical reaction sample tube module comprises a metal heat conductive block base; The metal heat-conducting block base is arranged on the temperature control module, and a plurality of first reaction bottles are placed on the metal heat-conducting block base, and a strong magnetic stirring bar 1 is placed in each of the first reaction bottles; The electrode mounting substrate is arranged on the top of the first reaction bottle corresponding to the metal heat conductive block base, one end of each of the electrodes is mounted on the electrode mounting substrate, and the other end extends into the corresponding first reaction bottle respectively, and the electrodes are energized through the electrode control bus.

3. The modular high-throughput parallel chemical reaction device according to claim 2, characterized in that: The light source module includes a dot matrix light source board, a light source control bus and light source lamp beads; The photoreaction sample tube module comprises a metal base; The metal base is arranged on the dot matrix light source board, and a plurality of second reaction bottles are placed on the metal base. A strong magnetic stirrer 2 is placed in each of the second reaction bottles. A light source lamp bead is arranged on the dot matrix light source board at a position corresponding to each of the second reaction bottles, and the light source lamp bead is used to illuminate the chemical reagents in the second reaction bottles.

4. The modular high-throughput parallel chemical reaction device according to claim 3, characterized in that: The metal base has a built-in microporous channel 1, and a cooling liquid inlet and outlet are provided on the side wall of the metal base, and the cooling liquid inlet and outlet are used to pass a heat-conducting or cooling-conducting fluid, and the heat-conducting or cooling-conducting fluid controls the temperature of the photochemical reaction in the second reaction bottle through the microporous channel 1; The dot matrix light source board has a built-in microporous channel 2, and a cooling liquid inlet and outlet are arranged on the side wall of the dot matrix light source board. The cooling liquid inlet and outlet are used to pass cooling liquid, and the cooling liquid enters the microporous channel 2 to cool the light source lamp beads.

5. The high-throughput parallel chemical reaction device with modular design according to any one of claims 1 to 4, characterized in that: The temperature control module includes a temperature control base, and a plurality of the temperature control bases are located directly above the vertical magnetic line generating module and are arranged in sequence along the direction of the vertical magnetic line generating module. The temperature control bases are connected to a temperature controller.

6. The modular high-throughput parallel chemical reaction device according to claim 3 or 4, characterized in that: The outer dimensions of the sample reaction tube module, the metal heat conductive block base and the metal base are consistent with the SLAS / SBS ​​standard well plate dimensions, and the interiors are designed to be 96 wells 1 ml, 48 wells 2 ml, 24 wells 4 ml or 8 ml.

7. The modular high-throughput parallel chemical reaction device according to claim 5, characterized in that: The vertical magnetic field line generating module includes a magnetic pole group and a fixing frame; The fixing frame is arranged outside the magnetic pole group and is used to fix the magnetic pole group; The magnetic pole set includes a connecting frame and one or more magnets; The connecting frame is arranged inside the fixing frame and is parallel to the plane where the bottom surface of the temperature control module is located; One magnet is fixed on the connecting frame alone; or a plurality of magnets are evenly and evenly fixed on the connecting frame at equal intervals, and a line connecting two magnetic poles of each magnet is perpendicular to the bottom surface of the temperature control module.

8. The modular high-throughput parallel chemical reaction device according to claim 7, characterized in that: A driving module is also provided in the vertical magnetic stirring mixing platform. The driving module is connected to the vertical magnetic force line generating module and is used to drive the vertical magnetic force line generating module to rotate in a plane perpendicular to the bottom surface of the temperature control module.

9. The modular high-throughput parallel chemical reaction device according to claim 8, characterized in that: The driving module includes a driving motor, a coupling and a speed controller; The output shaft of the driving motor is connected to the connecting frame, the speed controller is connected to the driving motor, and the connecting frame is connected to the output end of the driving motor through the coupling.

10. The modular high-throughput parallel chemical reaction device according to any one of claims 7 to 9, characterized in that: The vertical magnetic stirring mixing platform also includes a host control screen, which is arranged on the front of the vertical magnetic stirring mixing platform; The magnet is a neodymium iron boron magnet.

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